Preparation method and application of fish feed antioxidant based on ginkgo leaf extracts and application
A technology of antioxidants and extracts, applied in the preparation and application of fish feed antioxidants based on ginkgo biloba extracts, can solve problems such as toxic and side effects, and achieve the effect of increasing growth rate and feed intake
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Embodiment 1
[0039] A method for applying ginkgo biloba extract as fish feed antioxidant, the following steps are carried out in sequence:
[0040] 1. Obtain 1000 g of fresh ginkgo leaves, dry them in a blast drying oven at 50° C. to constant weight, crush them through a 18-mesh sieve, and obtain dry ginkgo leaves powder.
[0041] 2. Take 4 parts of ginkgo leaf powder, 200g each, and put them into 4 containers. According to the mass ratio, add petroleum ether, ethyl acetate, ethanol and distilled water at a ratio of 1:9 between ginkgo leaf powder and solvent, and use a rotary agitator to Mix and stir at room temperature at 800rpm / min for 6 hours, and repeat the extraction 3 times. Filter the mixture with Bush funnel and medium-speed qualitative filter paper to obtain a clear residue-free filtrate. The filtrates were respectively placed in a rotary evaporator and distilled under reduced pressure to constant weight to remove the solvent to obtain Ginkgo biloba petroleum ether extract, Ginkg...
Embodiment 2
[0058] On the basis of Example 1, continue to operate as follows:
[0059] 4. According to the feed formula, 4 kinds of extracts of Ginkgo biloba were added to feed raw materials at concentrations of 0 and 1 g / kg feed and mixed to make pellet feed with a diameter of 2 mm.
[0060] Feed formula:
[0061]
[0062] In this feed formula, fish meal is a self-made raw material without any additives;
[0063] Soybean meal content ≥ 99%, food grade;
[0064] Among the vitamin supplements, each kilogram of vitamin supplements contains: retinol (500,000IU / g) 0.80g, vitamin D 3 (500,000IU / g) 0.48g, DL-α-tocopherol (50%) 20.00g, vitamin K 3 (23%) 0.43g, Vitamin B 1 (90%) 0.11g, riboflavin (80%) 0.63g, pyridoxine (81%) 0.92g, cyanocobalamin (1%) 0.10g, D-calcium pantothenate (90%) 2.73g, niacin (99%) 2.82g, D-biotin (2%) 5.00g, inositol (99%) 52.33g and folic acid (96%) 0.52g;
[0065] In mineral additives, each kilogram of mineral additives contains: FeSO 4 ·7H 2 O (containing ...
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