Unlock instant, AI-driven research and patent intelligence for your innovation.

Peach transcription factor pphb G7 gene, protein, recombinant expression vector and application thereof

A gene and application technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problem of HB gene shortage, achieve the effects of extending shelf life, achieving environmental friendliness, and reducing agricultural costs

Active Publication Date: 2020-07-28
JIANGSU ACAD OF AGRI SCI
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are few studies on the role of HB genes in fruit development and ripening, and only the regulation of tomato LeHB-1 on fruit ripening has been reported.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Peach transcription factor pphb G7 gene, protein, recombinant expression vector and application thereof
  • Peach transcription factor pphb G7 gene, protein, recombinant expression vector and application thereof
  • Peach transcription factor pphb G7 gene, protein, recombinant expression vector and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Identification of the HB family of peach and other fruit trees

[0034] A large number of HB genes were isolated from the peach genome (version 1), and their nucleotide and amino acid sequences were downloaded from the Rosaceae Genome Database (GDR; http: / / www.rosaceae.org / ), and the obtained amino acids Sequences were screened to obtain members of the HB family in the peach genome. At the same time, pear (Pyrus bretschneideri; http: / / peargenome.njau.edu.cn / ), raspberry (Rubus occidentalis; GDR), strawberry (Fragaria × ananassa), citrus (Citrus sinensis), grape (Vitis vinifera), Search and download all members of the HB family from gene banks such as papaya (Carica papaya). Finally, all the genes found are in the National Center for Biotechnology Information (NCBI; https: / / www.ncbi.nlm.nih.gov)) Further comparison of their homology.

Embodiment 2

[0035] Example 2 Sequence and phylogenetic tree analysis

[0036] The amino acid sequences of the above-screened genes were downloaded and aligned by the Culstal X method, and a phylogenetic tree based on Mega-Weighted Neighbor-Joining (NJ) was constructed using the software MEGA6. Seventy HB genes were isolated from the peach genome, and together with the Hb genes in pear, strawberry, citrus, papaya and grape, a phylogenetic tree was constructed. The results showed that: 398 HB genes can be divided into two classes I and II, which have 5 groups (A-E) and 2 groups (F and G) ( figure 1 ). These genes were further divided into 67 clusters besides group D, namely A1→A20, B1, B2, C1→C3, E1→E13, F1→F10 and G1→G19. Sequence alignment in NCBI showed that group A contained three previously reported families, BEL (A1→A11), KNOX (A12→A18) and PHD (A19 and A20). Groups C, D and F were similar to the DDT, SAWDEE and WOX families, respectively. Group E contains two subfamilies HD-ZIP I...

Embodiment 3

[0043] Example 3 Expression profile of HB gene in peach fruit

[0044] According to the transcriptome data, 10 varieties including Baixianglu, Zaobaifeng, Chiyuanmi, Chunmei, Xiahui No. 2, Yuhualu, Chunxue, Baifeng, Hujing Milu and Xiahui No. 6 were used. The expression levels of 70 genes were analyzed at the transcriptional level at S1-S3) and mature (S4) stages. The results showed that the expression of two genes PpHB.G2 and PpHB.G7 during fruit ripening was higher than that during fruit development, while five genes PpHB.A11, PpHB.E1, PpHB.E12, PpHB.E13 and PpHB.F4 were expressed in The lowest expression level during fruit ripening ( figure 2 A). In order to verify the expression levels of these seven genes, qRT-PCR technology was used to detect the expression levels of these seven genes in the fruit development and ripening stages of Zhongyou No. 4 peach. The results showed that the expression levels of PpHB.A11, PpHB.E12, and PpHB.E13 were not significantly different ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a peach transcription factor PpHB.G7 gene which is a member of HB family. The nucleotide sequence of the peach transcription factor PpHB.G7 gene is shown by SEQ ID NO. 1 of thesequence table, thelength in the coding region sequence is 936bp, 311 amino acids are coded, and the amino acid sequence is shown by SEQ ID NO. 2 in the sequence table. The expression quantity of thePpHB.G7 gene in the fruit mature period is higher than that before the fruit maturation. According to biological function verification, the cloned PpHB.G7 gene in the invention has a function of promoting ethylene biosynthesis. The discovery of the PpHB.G7 gene provides a new gene resource for the molecular breeding of ethylene biosynthesis and a new genetic resource for implementing green agriculture; the development and utilization of the resource is favorable for improving the commercial value and market competitiveness of peach fruits, prolonging the shelf life of the fruits, reducing theagricultural cost and realizing environmental friendliness.

Description

technical field [0001] The invention relates to peach transcription factor PpHB.G7 gene, protein, recombinant expression vector and application thereof, and belongs to the field of plant genetic engineering. Background technique [0002] Fruit ripening refers to a complex and orderly series of physiological and biochemical reactions that occur after fruit growth and development stops, involving color, flavor, aroma, texture, etc., and fruit ripening directly affects the commercial value, post-harvest storage and market of the fruit. Competitiveness. (Giovannoni et al, 2004; Li et al, 2010; Tian Shiping, 2013). Fruit ripening has been extensively studied in tomatoes, apples, bananas, peaches, etc. Fruits are classified into climacteric and non-climacteric types according to whether there is a respiration peak during ripening (Leli E Vre J, 1997). For climacteric fruit, a large amount of ethylene is released during fruit ripening, and exogenous ethylene treatment can promot...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/82C12N15/11C07K14/415A01H5/00A01H6/74
CPCC07K14/415C12N15/8249
Inventor 张妤艳谷超马瑞娟
Owner JIANGSU ACAD OF AGRI SCI