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Full bacteria saccharification method for lignocellulose

A technology of lignocellulose and bacterial sugar, applied in the biological field, can solve the problems of shortening the period of seed culture, high cost of seed liquid preparation, shortening the saccharification cycle of whole bacteria, etc. Effect of Fibrosome Specific Viability

Inactive Publication Date: 2018-12-21
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Aiming at the problems of long period and high cost of seed liquid preparation in lignocellulose whole-bacteria saccharification in the prior art, the present invention provides a whole-bacteria saccharification method for lignocellulose, which not only greatly shortens the time spent in the seed culture stage cycle, which reduces the cost of seed liquid and shortens the cycle of saccharification of whole bacteria, thus laying the foundation for the process of industrialization

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  • Full bacteria saccharification method for lignocellulose

Examples

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Effect test

Embodiment 1

[0023] The whole bacteria saccharification method for lignocellulose comprises the following steps:

[0024] (1) Seed liquid optimization:

[0025] Under anaerobic conditions, Clostridium thermocellum was subcultured for 3 times according to the inoculation ratio of 1% volume fraction in the medium with 5g / L glucose as the only carbon source, i.e. glucose medium; then inoculated into Add 0.1g / L dry weight of pretreated corn stalks to the glucose medium, and induce acclimatization at 55°C for 48 hours, so as to obtain whole bacteria with high growth rate, high cellulite production, and high cellulite specific activity Saccharified seed liquid. In addition to containing 5g / L glucose, the glucose culture medium also has 2.9g / L of dipotassium hydrogen phosphate, 1.5g / L of potassium dihydrogen phosphate, 0.8g / L of urea, and 0.1g / L of calcium chloride in every liter of water. , magnesium chloride 1.8g / L, ferrous sulfate 0.0005g / L, sodium sulfide 2g / L, corn steep liquor 4g / L, triso...

Embodiment 2

[0031] Different from Example 1, the whole bacteria saccharification method for lignocellulose, without domestication and pre-soaking steps, includes the following steps:

[0032] (1) Seed solution culture: Clostridium thermocellum was cultured for 48 hours in a medium (glucose medium except glucose) that used 5 g / L pretreated corn stalks as a carbon source. After step (1), the method of Example 1 was used to detect the specific activity of cellulite, yield and cell biomass of the seed liquid.

[0033] (2) Whole-bacteria saccharification: according to the inoculum amount of 10% volume fraction, the seed solution is directly inoculated into the reaction system containing 1 kg of pretreated corn stalks and 6.5 L of medium solution to carry out whole-bacteria saccharification, pretreated raw materials and The conditions for the saccharification of whole bacteria were the same as in Example 1. In this process, every 12 hours sampling detects the content of reducing sugar in the f...

Embodiment 3

[0035] Different from Example 1, the whole bacteria saccharification method for lignocellulose comprises the following steps:

[0036] (1) Seed liquid optimization:

[0037] Under anaerobic conditions, Clostridium thermocellum was subcultured for 3 times in a medium with 10 g / L glucose as the only carbon source, i.e. glucose medium, according to the inoculation ratio of 1% volume fraction; then inoculated into Add 0.5g / L dry weight of pretreated wheat straw to the glucose medium, and induce domestication at 58°C for 36 hours, so as to obtain whole bacteria with high growth rate, high cellulite production, and high cellulite specific activity Saccharified seed liquid. The pretreatment method is the combined pretreatment method of hydrothermal and sulfonation method adopted in CN201610133959.

[0038] (2) Raw material pre-soaking: According to the solid-liquid weight-to-volume ratio of 1:5, 1 kg of pretreated lignocellulose raw material and 5 L of medium solution were mixed ev...

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Abstract

The invention provides a full bacteria saccharification method for lignocellulose. The full bacteria saccharification method comprises the following steps of (1) optimizing of a seed solution under the anaerobic condition, performing passage domestication on a cellulosome-producing strain on a culture medium using glucose as a unique carbon source; then, inoculating into a glucose culture medium added with the pretreated lignocellulose raw material, and performing inducing domestication; (2) pre-impregnating of raw materials: uniformly mixing the pretreated lignocellulose raw material and a culture medium solution; (3) full bacteria saccharification: inoculating the optimized seed solution of full bacteria saccharification in step (1) into a reaction system after pre-impregnating, and performing saccharification reaction, so as to obtain the solution containing the glucose. The full bacteria saccharification method has the advantages that the cycle in the seed culture phase is greatlyshortened, the cost of the seed solution is reduced, the full bacteria saccharification cycle is shortened, and a foundation is laid for the industrialization process.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a biotransformation method of lignocellulose, in particular to a whole-bacteria saccharification method for lignocellulose. Background technique [0002] In recent years, obtaining renewable energy, materials or chemicals from biomass has attracted widespread attention from all over the world. The use of lignocellulosic biomass as raw materials to produce second-generation bio-based fuels or chemicals has become the key to large-scale replacement of petroleum-based products. [0003] Lignocellulosic biomass is the most abundant renewable biomass resource on earth, and its conversion into energy, chemicals or materials has great application and development prospects. In particular, my country is a large agricultural country, with an annual crop straw output of more than 900 million tons. On the other hand, problems such as environmental pollution and traffic safety accidents caused by...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/02C12R1/145C12R1/01
CPCC12P19/02C12P19/14C12P2201/00C12P2203/00
Inventor 崔球刘亚君祁宽刘世岳李仁民冯银刚
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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