Preparation method of carbon nano-enzyme co-doped with active metal and nitrogen element and application thereof as nano biological probe for detecting hydrogen peroxide

A technology of active metals and nitrogen elements, applied in chemical instruments and methods, catalyst activation/preparation, physical/chemical process catalysts, etc., can solve problems such as poor catalytic activity, unfavorable large-scale production and commercial application of carbon nanozymes, etc. Achieve the effect of fast reaction speed, good enzyme catalytic activity and low temperature

Pending Publication Date: 2019-01-01
HENAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preparation of carbon nanozymes often uses hydrothermal and other methods, which is not conducive to the large-scale production and commercial application of carbon nanozymes.
At the same time, compared with transition metal oxide nanozymes and noble metal nanozymes, the catalytic activity of pure carbon nanozymes is relatively poor.

Method used

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  • Preparation method of carbon nano-enzyme co-doped with active metal and nitrogen element and application thereof as nano biological probe for detecting hydrogen peroxide
  • Preparation method of carbon nano-enzyme co-doped with active metal and nitrogen element and application thereof as nano biological probe for detecting hydrogen peroxide
  • Preparation method of carbon nano-enzyme co-doped with active metal and nitrogen element and application thereof as nano biological probe for detecting hydrogen peroxide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Step S1: Dissolve 7g of cobalt nitrate hexahydrate and 4.5g of disodium edetate in 20mL of deionized water respectively to form uniform cobalt nitrate solution and disodium edetate solution, then add the cobalt nitrate solution dropwise to Add 10 mL of methanol to the disodium EDTA solution under vigorous stirring, filter the resulting reaction solution, wash the obtained filter cake with deionized water to remove impurities, and dry at 60°C to obtain a solid powder;

[0024] Step S2: put the dried solid powder in step S1 into a quartz tube of a tube furnace, and thermally polymerize at 300° C. for 2 hours under a nitrogen atmosphere to obtain a brown-black product;

[0025] Step S3: Grind the brown-black product obtained by thermal polymerization and disperse it in deionized water under ultrasonic treatment, filter the supernatant brown solution after centrifugation with a microporous filter to remove large particles, and then dry it at 100°C after dialysis 6h to get a...

Embodiment 2

[0032] Step S1: Dissolve 20mg of nickel acetate and 4.5g of disodium edetate in 1mL and 10mL of deionized water respectively to form a uniform solution of nickel acetate and disodium edetate, then add the nickel acetate solution dropwise to Add 10 mL of ethanol to the disodium EDTA solution under vigorous stirring, filter the resulting reaction solution, wash the obtained filter cake with deionized water to remove impurities, and dry at 60°C to obtain a solid powder;

[0033] Step S2: put the dried solid powder in step S1 into a quartz tube of a tube furnace, and thermally polymerize at 450° C. for 30 minutes under a nitrogen atmosphere to obtain a brown-black product;

[0034] Step S3: Grind the brown-black product obtained by thermal polymerization and disperse it in deionized water under ultrasonic treatment, filter the supernatant brown solution after centrifugation with a microporous filter to remove large particles, and then dry it at 180°C after dialysis 1.5h to obtain ...

Embodiment 3

[0036]Step S1: Dissolve 0.2g of cerium sulfate and 4.5g of disodium edetate in 5mL and 20mL of deionized water respectively to form a uniform solution of cerium sulfate and disodium edetate, then add the cerium sulfate solution dropwise Add 10 mL of methanol to the disodium edetate solution under vigorous stirring, filter the resulting reaction solution, wash the obtained filter cake with deionized water to remove impurities, and dry at 60°C to obtain a solid powder;

[0037] Step S2: put the dried solid powder in step S1 into a quartz tube of a tube furnace, and thermally polymerize at 180° C. for 8 hours under a nitrogen atmosphere to obtain a brown-black product;

[0038] Step S3: Grind the brown-black product obtained by thermal polymerization and disperse it in deionized water under ultrasonic treatment, filter the supernatant brown solution after centrifugation with a microporous filter to remove large particles, and then dry it at 60°C after dialysis 10h to obtain the c...

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Abstract

The invention discloses a preparation method of a carbon nano-enzyme co-doped with active metal and nitrogen element and application thereof as a nano biological probe for detecting hydrogen peroxide.The method comprises the steps that: EDTA disodium and active metal are used as precursors, and are chelated to obtain an intermediate, the intermediate is subjected wot thermal polymerization underan inert atmosphere, and the reactant is ground, dispersed in water, ultrasonicated, filtered, dried to finally obtain the carbon nano-enzyme co-doped with the active metal and the nitrogen element. The method is simple and convenient to operate, and the large-scale preparation of carbon fluorescent quantum dots can be co-doped with the active metal and the nitrogen element without harsh reactionconditions, obtained carbon dots have good dispersibility and wider fluorescence emission range in aqueous solution. The nano-enzyme can be applied to the detection of hydrogen peroxide as a fluorescent probe, and has potential application value in the fields of medical imaging and detection of other biomolecules.

Description

technical field [0001] The invention belongs to the technical field of nanozyme synthesis, and in particular relates to a preparation method of a carbon nanozyme co-doped with active metals and nitrogen elements and its application as a nanobiological probe to detect hydrogen peroxide. Background technique [0002] Nanozymes are a class of nanomaterials with enzyme-like catalytic activity. Natural enzymes are sensitive to temperature, acidity and alkalinity, easy to inactivate, difficult to purify, difficult to preserve, and high cost, which affect their wide use in industrial production and scientific research. Therefore, researchers have been working on the development of nanozymes that can overcome the above defects. Since Fe 3 o 4 Since magnetic nanoparticles were reported to have peroxidase-like activity, the construction of nanozymes has become a research hotspot in the past decade. Compared with natural enzymes, these nanozymes have the advantages of higher stabil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J27/24B01J35/02B01J37/08G01N21/64G01N21/78
CPCB01J27/24B01J35/023B01J37/082B01J37/084G01N21/6428G01N21/78G01N2021/6432
Inventor 苏莉蔡晔茜王亮仉华毛国江董文佩康朴华
Owner HENAN NORMAL UNIV
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