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Dual-emitting cellulose-based fluorescent material, preparation method and applications thereof

A fluorescent material and cellulose-based technology, which can be used in luminescent materials, luminescent coatings, fluorescence/phosphorescence, etc., can solve the problems of limited practical application, inability to use materials alone, and low measurement accuracy

Active Publication Date: 2019-01-01
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of these fluorescent sensors in the past used a single fluorescent molecule to monitor
In the process of use, it is very easy to be interfered by factors such as the concentration of fluorescent molecules, the external environment, and instrument parameter settings, which makes the measurement accuracy low
At the same time, although the fluorescence intensity changes produced by a single fluorescent molecule during the detection process can also be observed with the naked eye, it is impossible to establish a unified observation standard, because the naked eye's ability to perceive light intensity is greatly affected by human factors
In addition, the fluorescent sensors used today are based on small molecular compounds and cannot be used as materials alone
At the same time, small molecular fluorescent compounds are easy to migrate with environmental changes, and have high toxicity, which limits their practical application.

Method used

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  • Dual-emitting cellulose-based fluorescent material, preparation method and applications thereof
  • Dual-emitting cellulose-based fluorescent material, preparation method and applications thereof
  • Dual-emitting cellulose-based fluorescent material, preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] Preparation of Dual Emission Cellulose-Based Fluorescent Materials by Twice Esterification

[0085] Weigh 1.9g cellulose acetate butyrate and 0.016g fluorescein isothiocyanate to carry out esterification reaction under the catalytic conditions of dibutyltin dilaurate (esterification reaction temperature is 100°C; esterification reaction time is 4h) , the obtained product was precipitated, washed and dried, then dissolved again, and subjected to an esterification reaction with 0.11 g of protoporphyrin under the catalysis of carbonyldiimidazole (the esterification reaction temperature was 80° C.; the esterification reaction time was 20 h).

[0086] The fluorescent group of fluorescein isothiocyanate and the fluorescent group of protoporphyrin in the double-emission cellulose-based fluorescent material are bonded on the same cellulose, wherein the fluorescent group of fluorescein isothiocyanate is bound to the biogenic amine Responsive, protoporphyrin fluorophore is not re...

Embodiment 2

[0089] Preparation of dual-emission cellulose-based fluorescent materials by solid blending method

[0090] Weigh 0.9478g cellulose acetate and 0.0389g fluorescein isothiocyanate to carry out esterification reaction under the catalytic condition of dibutyltin dilaurate (esterification reaction temperature is 100 ℃; esterification reaction time is 4h), will The obtained product is precipitated, washed and dried for later use.

[0091] Weigh 0.9478g cellulose acetate and 0.0563g protoporphyrin to carry out esterification reaction under the catalysis of carbonyldiimidazole (esterification reaction temperature is 80°C; esterification reaction time is 20h), the obtained product is precipitated, washed and dried for backup use.

[0092] Mix the cellulose and its derivatives bonded to the isothiocyanate fluorescein fluorescent group and the cellulose and its derivatives bonded to the protoporphyrin fluorescent group with a mass ratio of 1:5 , and ground in a mortar to prepare a dou...

Embodiment 3

[0098] Preparation of Dual Emission Cellulose-Based Fluorescent Materials by Solution Blending

[0099] Weigh 0.9478g of cellulose nitrate and 0.0389g of fluorescein isocyanate to carry out esterification reaction under the catalytic conditions of dibutyltin dilaurate (esterification reaction temperature is 100°C; esterification reaction time is 4h), and the resulting product is precipitated , Wash and dry for later use.

[0100]Weigh 0.9478g of methylcellulose and 0.0563g of protoporphyrin to carry out esterification reaction under the catalysis of carbonyldiimidazole (esterification reaction temperature is 80°C; esterification reaction time is 20h), and the resulting product is precipitated, washed and dried Backup.

[0101] Dissolve the cellulose derivatives bonded to the isocyanate fluorescein fluorescent group and the cellulose derivatives bonded to the protoporphyrin fluorescent group that were synthesized respectively in N,N-dimethylformamide, and then the above two T...

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Abstract

The invention discloses a dual-emitting cellulose-based fluorescent material, a preparation method and applications thereof, wherein the dual-emitting cellulose-based fluorescent material has good biocompatibility and good workability, and comprises two classes of fluorophores, wherein one class of the fluorophores are responsive to biogenic amines so as to be used as indicators, and the other class of the fluorophores are not responsive to biogenic amines so as to be used as internal standard substances. The invention further discloses a method for detecting biogenic amines, wherein the dual-emitting cellulose-based fluorescent material is used, and the biogenic amine is detected according to the change of the ratio of the fluorescence intensities of the two classes of the fluorophores and the change of the fluorescent color of the cellulose-based fluorescent material, and during the detecting, the ratio of the fluorescence intensities of the two classes of the fluorophores produces the visible color change along with the increase of the concentration of the biogenic amine so as to achieve the visual detection of biogenic amine.

Description

technical field [0001] The invention relates to a double-emission cellulose-based fluorescent material and its preparation method and application, belonging to the technical field of biological detection. Background technique [0002] Biogenic amines refer to a class of organic amine compounds, including ammonia, dimethylamine, trimethylamine, etc., which are closely related to various metabolic processes and are produced in large quantities during the degradation of amino acids. An abnormal increase in the concentration of biogenic amines is often taken as a signal of food spoilage or disease. Therefore, the development of an effective method for the detection of biogenic amines is crucial for its practical application. Recently, due to its unique high sensitivity and good selectivity, fluorescent sensors have made some research progress in the fields of food quality monitoring and auxiliary disease diagnosis. Benzhong Tang's group used the selective responsiveness of agg...

Claims

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Application Information

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IPC IPC(8): C09K11/06C08B15/00C08B3/00C08B3/18C08B3/06C08B5/02C08B11/00C08B11/12C08B11/02C09D5/22G01N21/64B65D65/42
Inventor 张军贾若男张金明
Owner INST OF CHEM CHINESE ACAD OF SCI
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