Method for adopting ultrasonic waves for assisting deep-eutectic solvent in extracting smilax china flavonoid compound

A technology of flavonoids and low eutectic solvent is applied in the field of deep processing of plant active ingredients, which can solve the problem of not much research on extracting active ingredients, and achieve the effects of simple preparation method and low cost.

Inactive Publication Date: 2019-01-04
QIANNAN NORMAL UNIV FOR NATTIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In summary, the current research on the use of DES to extract active ingredients has been gradually carried out, and has strong advantages in this aspe

Method used

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  • Method for adopting ultrasonic waves for assisting deep-eutectic solvent in extracting smilax china flavonoid compound

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Experimental program
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Embodiment 1

[0023] Preparation of deep eutectic solvent: choline chloride and glycerol were heated and stirred at 80° C. for 2 h under anhydrous conditions at a molar ratio of 1:2 until a colorless and transparent solution was obtained, and then sealed and preserved after cooling.

[0024] Use rutin as the standard product to develop a standard curve, and use the ultraviolet spectrophotometer to measure the absorbance of the deep eutectic solvent-treated rutin standard product. The specific operation steps are: weigh the rutin standard product, add 60% ethanol to dissolve it; The solution was added to 2 mL of deep eutectic solvent, vortexed for 5 min to mix well, filtered through a 0.45 μm organic filter membrane, and the absorbance was measured at a wavelength of 505 nm. Use the concentration of standard substance on the abscissa and the absorbance value on the ordinate to make a standard curve, see figure 1 ; The linear regression equation is: Y=0.030847X+0.004198, R2=0.9991 shows that ...

Embodiment 2

[0026] Adopt the deep eutectic solvent prepared by embodiment one to extract the flavonoids in the sarsaparilla sample, then utilize the ultraviolet spectrophotometer to measure the flavonoids concentration in the extract; Concrete steps are as follows:

[0027] a, raw material preparation: after the sarsaparilla is aired and dried naturally, the smilax sarsaparilla powder is obtained through crushing through a 40-mesh sieve;

[0028] b. Ultrasonic treatment: Put 2g of sarsaparilla powder obtained after passing through a 40-mesh sieve into a 100mL conical flask, add 40mL of a deep eutectic solvent with a water content of 50% to dissolve, and add 0.8g of Tween 80 to the deep eutectic In the solvent, after shaking well, at a temperature of 50°C, a power of 100W, and ultrasonic treatment for 30 minutes, the flavonoids in Smilax smilax are extracted into the deep eutectic solvent aqueous solution to obtain an extract containing flavonoids;

[0029] c. Centrifugal extraction: the o...

Embodiment 3

[0032] Adopt the deep eutectic solvent prepared in embodiment 1 to extract the flavonoids in the sarsaparilla sample, then utilize the ultraviolet spectrophotometer to measure the flavonoids concentration in the extract; Concrete steps are as follows:

[0033] a, raw material preparation: after the sarsaparilla is aired and dried naturally, the smilax sarsaparilla powder is obtained through crushing through a 60-mesh sieve;

[0034] b. Ultrasonic treatment: Put 2g of sarsaparilla powder obtained after passing through a 60-mesh sieve into a 100mL conical flask, add 40mL of a deep eutectic solvent with a water content of 50% to dissolve, and add 0.8g of Tween 80 to the deep eutectic In the solvent, after shaking well, at a temperature of 60° C., a power of 150 W, and ultrasonic treatment for 20 minutes, the flavonoids in Smilax smilax were extracted into an aqueous deep eutectic solvent solution to obtain an extract containing flavonoids.

[0035] c. Centrifugal extraction: the ...

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Abstract

The invention discloses a method for adopting ultrasonic waves for assisting a deep-eutectic solvent in extracting a smilax china flavonoid compound. The method comprises the following steps of preparation of raw materials, ultrasonic treatment and centrifugal extraction. Ultrasonic cavitation is adopted for damaging cell tissue, dissolution and diffusion of active materials in cells are promoted,the ultrasonic waves are combined with the deep-eutectic solvent, and the method is a green method for extraction and separation; moreover, the extraction rate of active components can be effectivelyincreased, thus plant resources are fully utilized, and the drug therapeutic effect is improved; meanwhile, the adopted the adopted preparing method through the deep-eutectic solvent is simple and low in cost, and favorable conditions are provided for industrialized application of extraction of active components of Chinese herbal medicine.

Description

technical field [0001] The invention relates to the technical field of deep processing of plant active ingredients, in particular to a method for extracting smilax flavonoids with ultrasonic-assisted deep eutectic solvents. Background technique [0002] Sarsaparilla is the dry rhizome of Smilax genus Liliaceae, also known as King Kongteng, which is a species recorded in the 2010 edition of "Chinese Pharmacopoeia". It is mainly distributed in the south of the Yangtze River. As one of the commonly used medicinal materials in ethnic minority areas in Guizhou, it has the effects of treating dysentery, women's inflammation, rectal cancer, diabetes, etc.; abroad, such as: Pakistani folks are used to treat rheumatism and asthma. Sarsaparilla has a wide range of therapeutic effects, mainly due to its rich active ingredients, such as saponins, flavonoids, sterols, etc. Among them, there have been many reports on the efficacy of flavonoids, and related pharmacological studies have sh...

Claims

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Application Information

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IPC IPC(8): A61K36/896B01D11/02B01J19/10A61K125/00
CPCA61K36/896A61K2236/15A61K2236/331A61K2236/39A61K2236/51A61K2236/53B01D11/0265B01D11/0288
Inventor 黄德娜曾承露代虹镜李锋
Owner QIANNAN NORMAL UNIV FOR NATTIES
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