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Lactobacillus acidophilus La-SJLH001 with probiotic function of regulating blood sugar and cholesterol level and application thereof

A Lactobacillus acidophilus and probiotic technology, applied in the field of microbial engineering, can solve the problems of unstable number of viable bacteria in lactic acid bacteria products, short product shelf life, dependence on imports of strains, etc. Acceptable and good amylase inhibitory activity

Active Publication Date: 2019-01-11
BEIJING SHOU JIA LI HUA SCI TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are various types of lactic acid bacteria-related products on the market, including yogurt, lactic acid beverages, single-compound bacteria food, health care products, etc., but most of the product strains are dependent on imports, and there are unstable viable counts of lactic acid bacteria products, fuzzy functions, and short shelf life. question

Method used

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  • Lactobacillus acidophilus La-SJLH001 with probiotic function of regulating blood sugar and cholesterol level and application thereof
  • Lactobacillus acidophilus La-SJLH001 with probiotic function of regulating blood sugar and cholesterol level and application thereof
  • Lactobacillus acidophilus La-SJLH001 with probiotic function of regulating blood sugar and cholesterol level and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Screening, identification and preservation of strains producing bile salt hydrolase

[0033] 1. Sample collection: Collect a variety of traditional Chinese fermented foods (sauerkraut, tempeh, fermented bean curd, etc.) and store them in cold storage.

[0034] 2. Isolation and purification of strains: add 1 g of the collected sample to 9 mL of sterile normal saline, mix well, take 1 mL of suspension and add 9 mL of sterile normal saline, and then dilute a series of ten times to obtain the respective concentrations as 10 -6 , 10 -7 And 10 -8 Each 0.1 mL of the bacterial solution was coated on the MRS plate, and each dilution was made three replicates. The anaerobic culture was carried out at 37 ºC for 48 h, and a single colony was selected for streaking anaerobic culture.

[0035] 3. Screening of bile salt hydrolase-producing strains: After streaking for 48 h, the purified single colony was inoculated into MRS liquid medium and cultured for 24 h. Formulated with 5 m...

Embodiment 2

[0039] Example 2: Gastrointestinal tolerance test of Lactobacillus acidophilus La-SJLH001

[0040] 1. Tolerance measurement of artificial gastric juice: Take 5 mL of activated Lactobacillus acidophilus La-SJLH001 culture solution, centrifuge (5000g, 10 min) to collect the bacterial cells, wash twice with PBS buffer, and make a bacterial suspension. Add 1 mL of bacterial suspension to 9 mL of artificial gastric juice (NaCl 0.2%, pepsin 0.35%, adjust the pH to 3.0 with 1 N HCl, filter and sterilize), place it at 37 °C anaerobic culture, respectively at 0 h, 1 h Samples were taken at 2 h and 3 h, and the number of viable bacteria was determined by the plate counting method. The 0 h sample was used as a control to calculate the survival rate of the strain at 1 h, 2 h and 3 h. Survival rate (%) = 1 h or 2 h or 3 h viable bacteria count / 0 h viable bacteria count × 100%.

[0041] The experimental results are as Figure 4 Shown. The results prove that Lactobacillus acidophilus La-SJLH001...

Embodiment 3

[0046] Example 3: In vitro biological activity determination of Lactobacillus acidophilus La-SJLH001

[0047] 1. Determination of the antioxidant activity of Lactobacillus acidophilus: take 2 mL of La-SJLH001 samples of Lactobacillus acidophilus (bacteria suspension, fermentation supernatant and cell fragment supernatant, namely bacterial cells, extracellular secretions and intracellular substances ) Add 2 mL of DPPH with a concentration of 0.2 mM, adjust to zero with methanol as a blank, react for 20 min at 37 °C in the dark, and measure OD after centrifugation at 5000 g 519 Calculate the free radical scavenging rate. Oxidation resistance (%)=[1-(A-B) / C]×100%, where A: contains sample and DPPH solution; B: contains sample solution but does not contain DPPH solution; C: contains sample solution but contains DPPH solution.

[0048] The experimental results are as Figure 7 Shown. The results proved that the anti-oxidant properties of the La-SJLH001 suspension (i.e. bacteria), ferme...

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Abstract

The invention relates to a self-screened Lactobacillus acidophilus SJLH001 (La SJLH001 for short) with probiotic function of regulating blood sugar and cholesterol level and application thereof in probiotic food. The strain has good tolerance to gastrointestinal environment, and has antioxidant, hydrophobic, ultrasonic resistance, reducing power and other in vitro benefits. In vitro and in vivo functional tests showed that La-SJLH001 has significant amylase inhibitory activity and alpha-Glucose glycerase inhibitory activity, and can significantly reduce fasting blood glucose, postprandial blood glucose level, enhance the tolerance of glucose, alleviate the pancreatic islet enlargement, reduce serum cholesterol level, enhance intestinal mucosal protection and other functions. La- SJLH001 has significant hypoglycemic and cholesterol-lowering activities, which makes it widely used in various forms of probiotic foods (such as probiotic tablet candy, probiotic solid drinks, etc.).

Description

Technical field [0001] The invention belongs to the field of microbial engineering, and relates to the separation, identification, activity detection, function verification, preparation of bacterial powder and its application in probiotics tableting candies and La-SJLH001 with probiotic functions such as regulating blood sugar and cholesterol levels. Application of probiotics in solid beverages. Background technique [0002] With the increasing improvement of people's living standards and changes in lifestyles, excessive intake of high fat and high protein in the diet has caused increasingly serious problems such as cardiovascular and cerebrovascular diseases and obesity, becoming one of the main factors threatening people's health. Probiotics are living microorganisms and their metabolites used to improve the health of humans, animals and plants, and are the core functional factors that improve the balance of the host intestinal microflora. Studies have shown that probiotics ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L2/39A23L2/52A23L33/135A23G3/36A23P10/28C12R1/23
CPCA23G3/366A23L2/39A23L2/52A23L33/135A23P10/28C12N1/20A23V2002/00C12R2001/23C12N1/205A23V2400/113A23V2200/328A23V2200/3262
Inventor 万向元孙倩张勇刘欣洁李金萍
Owner BEIJING SHOU JIA LI HUA SCI TECH CO LTD
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