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Lactobacillus rhamnosus derived from human milk and application thereof

A technology of Lactobacillus rhamnosus and breast milk, which is applied in the field of microorganisms and can solve problems such as toxic and side effects

Active Publication Date: 2022-03-18
AUSNUTRIA DAIRY CHINA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current drugs for the treatment of diseases caused by cadmium poisoning have certain toxic and side effects.

Method used

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  • Lactobacillus rhamnosus derived from human milk and application thereof
  • Lactobacillus rhamnosus derived from human milk and application thereof
  • Lactobacillus rhamnosus derived from human milk and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: the screening of bacterial strain

[0023] 1. Sample collection and strain isolation

[0024] Before collecting samples, the nipples of healthy volunteers were strictly sanitized, and then about 10 mL of breast milk was collected and placed in sterile centrifuge tubes. Take 2 mL of breast milk sample and pour it on the MRS agar medium prepared in advance, shake it to make it spread on the surface of the medium, seal the culture dish with a sealing film, and cultivate it at 37°C for 24-48 hours until a single colony grows, and select the lactic acid bacteria. Typical morphology is a characteristic single colony of white or milky white.

[0025] 2. Strain screening

[0026] Streak inoculation of the isolated strains on MRS agar medium containing bromocresol purple, and culture at 37°C for 48h. After growing a single colony, select a single colony that turns the medium yellow, observe the shape of the bacteria under a microscope, and carry out subsequent e...

Embodiment 2

[0039] Example 2: Tolerance to simulated gastrointestinal conditions of Lactobacillus rhamnosus AUH2101

[0040] 1) Test of tolerance to simulated gastrointestinal conditions

[0041] Preparation of strain fermented yogurt: Brew milk powder at a ratio of 14.7% (wt / v), fully dissolve, sterilize at 110°C for 12 minutes, cool to room temperature (about 20°C), and divide the sterilized milk into 40mL tubes. After the 16 strains obtained from the screening were activated by anaerobic culture, they were inoculated into sterilized milk at an inoculation amount of 3%, fermented at 37°C for about 4 hours, and then overnight at 4°C. The content of lactic acid bacteria was 10 9 cfu / ml.

[0042] Preparation of simulated saliva: 6.2g / L NaCl, 2.2g / L KCl, 0.22g / L CaCl 2 ,1.2g / L NaHCO 3 ;

[0043] Preparation of simulated gastric juice: 0.21g NaCl, 80.2mg pepsin, adjust the pH to 5.0 with concentrated hydrochloric acid, adjust the volume to 100ml with double distilled water, the activity ...

Embodiment 3

[0050] Example 3: Testing of bacterial strains for antibiotic resistance

[0051] With reference to the implementation standard of EFSA microbial anti-drug susceptibility test, the six strains screened and CECT5716 (Lactobacillus fermentum CECT5716 strain) were tested for drug resistance in this embodiment, and the results are shown in Table 3.

[0052] table 3

[0053]

[0054] Note: S means sensitive; I means intermediate; R means resistant.

[0055] It can be seen from Table 3 that there are differences in the drug resistance of each strain. Among them, strain LHL1 is sensitive to penicillin G and roxithromycin, strain LHL7 is sensitive to penicillin G and roxithromycin, strains LHL2, LHL3, LHL6 and AUH2101 are sensitive to penicillin G, tetracycline and roxithromycin at the same time, and have good safety sex. In addition, strain AUH2101 was also sensitive to azithromycin. Compared with the approved strain CECT5716 for infants, the Lactobacillus rhamnosus AUH2101 pr...

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Abstract

The invention relates to the technical field of microorganisms, and discloses a breast milk-derived Lactobacillus rhamnosus and an application thereof. The Lactobacillus rhamnosus preservation number of the present invention is CGMCC NO.21815. The Lactobacillus rhamnosus AUH2101 provided by the present invention is derived from healthy breast milk in China, and can not only produce two kinds of bacteriostatic substances, lactic acid and hydrogen peroxide, at higher concentrations, is sensitive to most antibiotics, but also has the ability to tolerate gastrointestinal conditions and Good heavy metal cadmium tolerance and removal ability, which in turn makes it have good antibacterial potential, low potential risk and good cadmium discharge function, and is expected to be developed and applied in the food industry.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a breast milk-derived Lactobacillus rhamnosus and an application thereof. Background technique [0002] Breast milk is considered to be the best source of nutrition for infant growth and plays a vital role in the growth and development of infants. A large number of studies have confirmed that breast milk contains a variety of microorganisms, including probiotics such as lactic acid bacteria and bifidobacteria, which have become an important source of probiotics. At present, there are relatively few probiotics derived from breast milk, such as Lactobacillus fermentum CECT5716 and Lactobacillus salivarius CECT5713, etc. These strains all show certain probiotic functions, and it is necessary to continuously explore probiotics from breast milk. [0003] Cadmium is a heavy metal that is extremely harmful to human health. It exists widely and stably in the environment and is th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L33/135A23L11/00A23L11/50A23C9/152A23C9/123A23L2/38A23L7/10A23L19/00A61K35/747A61P1/00A61P31/00C12R1/225
CPCC12N1/20A23L33/135A23L11/00A23C9/152A23C9/1234A23L2/382A23L7/198A23L19/01A61K35/747A61P1/00A61P31/00A23V2002/00A23V2400/175A23V2200/30
Inventor 康文丽汪家琦周洪波潘丽娜颜卫彬李辉宇戴智勇
Owner AUSNUTRIA DAIRY CHINA
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