A compound microbial agent for high-temperature compost organic fertilizer of animal manure and straw and a preparation method thereof

A technology for compounding microbial inoculants and livestock and poultry manures is applied in the biological field, which can solve the problems of low efficiency, low fermentation temperature, and inability to completely kill pathogenic bacteria and parasite eggs.

Active Publication Date: 2019-01-11
BIOTECH RES CENT SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The above patent documents using straw and livestock and poultry manure to make organic fertilizers all have the problem that the fermentation temperature is low, and the pathogenic bacteria and parasite eggs cannot be completely killed. At the same time, the fermentation cycle is long and the efficiency is low.
Moreover, the above-mentioned patent documents only use simple expansion technology to propagate strains, and do not play the role of strains in high-temperature fermented manure and straw

Method used

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  • A compound microbial agent for high-temperature compost organic fertilizer of animal manure and straw and a preparation method thereof
  • A compound microbial agent for high-temperature compost organic fertilizer of animal manure and straw and a preparation method thereof
  • A compound microbial agent for high-temperature compost organic fertilizer of animal manure and straw and a preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Bacillus siamensis (Bacillus siamensis) HT01 was obtained from the General Microbiology Center of China Committee for the Collection of Microbial Cultures, and the strain number was CGMCC 14115.

[0064] Bacillus licheniformis (Bacillus licheniformis) SDZD02 was obtained from the General Microbiology Center of China Committee for Culture Collection of Microorganisms, and the strain number was CGMCC 10682.

[0065] Aspergillus niger (Aspergillus niger) YF2501 was obtained from the General Microbiology Center of China Committee for Culture Collection of Microorganisms, and the strain number was CGMCC 15882.

[0066] A method for preparing a compound microbial bacterial agent for high-temperature composting of livestock and poultry manure and straw into organic fertilizer, the steps are as follows:

[0067] (1) Take Bacillus siamica, inoculate it in the seed medium, and cultivate it in a shaking flask with a shaker speed of 180rpm at 37°C for 12h to obtain the first-grade ...

Embodiment 2

[0081] As described in Example 1, a method for preparing a compound microbial bacterial agent for composting livestock and poultry manure and stalks into organic fertilizers at high temperature, the difference is that:

[0082] In described step (1), fermentation culture step is as follows:

[0083] The secondary seed culture solution of Bacillus siamese was inoculated in 6 tons of fermentation medium I with a tank capacity of 10 tons according to an inoculation amount of 2% by volume, the temperature was 37°C, the ventilation rate was 1: 1.5, and the tank pressure was 0.02MPa. Stirring speed 140rpm, culture 20h. The spore forming rate of the thalline was 95%, and the cultivation was stopped to obtain a Bacillus siamese fermentation broth.

[0084] Described fermentation medium I component is as follows, is weight part:

[0085] 4 parts of corn flour, 5.5 parts of bean cake powder, 2 parts of bran powder, 0.05 parts of sodium chloride, 0.01 part of magnesium chloride, 0.01 p...

Embodiment 3

[0095] As described in Example 1, a method for preparing a compound microbial bacterial agent for composting livestock and poultry manure and stalks into organic fertilizers at high temperature, the difference is that:

[0096] In described step (1), fermentation culture step is as follows:

[0097] The Bacillus siamese secondary seed culture solution was inoculated in 6 tons of fermentation medium I with a tank capacity of 10 tons according to an inoculation amount of 4% by volume, at a temperature of 38° C., a ventilation rate of 1: 1.4, and a tank pressure of 0.02 MPa. Stirring speed 160rpm, culture 24h. The spore-forming rate of the thalline was 90%, and the cultivation was stopped to obtain a Bacillus siamese fermentation broth.

[0098] Described fermentation medium I component is as follows, is weight part:

[0099] 4 parts of corn flour, 5 parts of bean cake powder, 2.5 parts of bran powder, 0.04 parts of sodium chloride, 0.01 part of magnesium chloride, 0.02 parts o...

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Abstract

The invention relates to a compound microbial agent for high-temperature compost organic fertilizer of animal manure and straw and a preparation method thereof. The compound microbial agent comprises,by weight, 20 to 30 part of Siamese bacillus preparation, 40 to 60 parts of licheniformis preparation and 10 to 20 part of aspergillus niger powder preparation, wherein that composite microbial agentcomprises 20 to 30 parts of Siamese bacillus preparation, 40 to 60 part of licheniformis preparation and 10 to 20 parts of aspergillus niger powder preparation. A composite microbial inoculum is obtained by uniformly mixing the microbial inoculum. The compound microbial agent prepared by the invention is suitable for innocuous treatment and resource utilization of waste resources produced in agricultural production, such as livestock manure and straw, for high-temperature compost organic fertilizer. The fermentation period is shortened and the treatment efficiency is improved; Kill pathogensand parasite eggs thoroughly, improve the maturity of fertilizer. Removal of fecal odor in the fermentation process to reduce the impact of fermentation on the surrounding environment.

Description

technical field [0001] The invention specifically relates to a compound microbial bacterial agent for high-temperature composting of livestock and poultry manure and straw into organic fertilizer and a preparation method thereof, belonging to the field of biotechnology. Background technique [0002] my country is a traditional large agricultural country, and the breeding industry is developing rapidly towards scale and intensification, while producing a large amount of livestock and poultry manure. According to statistics, the production of livestock and poultry manure in the country is as high as 3.8 billion tons, and the effective utilization rate is less than 50%. Environmental problems caused by livestock and poultry manure are becoming more and more serious. Since the improper disposal of livestock and poultry manure can cause many environmental problems, it is of great significance to treat it harmlessly. At present, the harmless treatment methods of livestock and po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C05F15/00C05F17/00C12R1/07C12R1/10C12R1/685
CPCC12N1/14C12N1/20C05F3/00C05F17/00C05F11/00C05F11/08Y02W30/40
Inventor 岳寿松边斐张燕张玉凤齐自成李福欣王翠萍
Owner BIOTECH RES CENT SHANDONG ACADEMY OF AGRI SCI
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