A kind of bacterial cellulose facial mask with antioxidant capacity and preparation method thereof

A technology of bacterial cellulose and anti-oxidation ability, applied in biochemical equipment and methods, methods based on microorganisms, medical preparations containing active ingredients, etc., can solve the problems affecting the large-scale industrial production of bacterial cellulose and the carbon source of culture medium High cost, unsatisfactory effect and other problems, to achieve the effect of high value utilization, inhibition of formation and deposition, and good inhibition effect

Active Publication Date: 2021-01-19
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the production cost of bacterial cellulose is high, especially the cost of the carbon source of the culture medium is high, and the unsatisfactory effect is an important factor affecting the large-scale industrial production of bacterial cellulose.
Milk is rich in vitamins, proteins, amino acids and trace elements, and has the effect of skin care and beauty. Due to the limitation of the characteristics of milk, its fresh-keeping period is usually short. At present, there is no systematic report on the treatment of expired milk.
[0003] Using bacterial cellulose film as a base material to prepare functional facial masks is a current research hotspot. Hainan Coslight Biotechnology Co., Ltd. discloses a method of cultivating bacterial cellulose with an ethanol solution containing total plant flavonoids as a nutrient solution to prepare a radiation-resistant mask. The method of facial mask (2013, 201310314954.5), but compared with the content of total flavonoids, the content of tea polyphenols has a stronger correlation with the total reducing ability, and the inhibitory ability to tyrosinase activity is also stronger, and different The properties of pure tea polyphenols are also different

Method used

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  • A kind of bacterial cellulose facial mask with antioxidant capacity and preparation method thereof
  • A kind of bacterial cellulose facial mask with antioxidant capacity and preparation method thereof
  • A kind of bacterial cellulose facial mask with antioxidant capacity and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Take 5g of pure milk, 0.1g of tea polyphenols, 5g of mannitol, 5g of peptone, 5g of yeast powder, MgSO 4 ·7H 2 Add 1000mL of distilled water to fully dissolve O 1g, adjust the pH value to 6.8 with 0.1mol / L NaOH and 0.1mol / L HCl solution, put it in a high-temperature sterilization pot at 121°C for 20min, cool it until it is not hot, add 5ml of absolute ethanol, Continue cooling to room temperature to obtain a fermentation medium. Take 100ml of fermentation medium and put it into 250ml sterilized Erlenmeyer flasks, let it stand for 12 hours, and it can be used only if no bacteria grow out. The activated Acetobacter xylinum suspension (about 1 × 10 7 cells / mL) were inoculated on the aliquoted fermentation medium in an amount of 5% (v / v), and cultured in a constant temperature and humidity chamber at 30° C. for 5 days.

[0038] Control group 1: take glucose 5g, mannitol 5g, peptone 5g, yeast powder 5g, MgSO 4 ·7H 2 Add 1000mL of distilled water to fully dissolve O 1g, ...

Embodiment 2

[0043] Take 30 days expired pure milk 10g, tea polyphenols 0.2g, mannitol 5g, peptone 5g, yeast powder 5g, MgSO 4 ·7H 2 Add 1000mL of distilled water to fully dissolve O 1g, adjust the pH value to 6.8 with 0.1mol / L NaOH and 0.1mol / L HCl solution, put it in a high-temperature sterilization pot at 121°C for 20min, cool it until it is not hot, add 5mL of absolute ethanol, Continue to cool to room temperature. Take 100mL of fermentation medium and put it into 250mL sterilized Erlenmeyer flasks, let it stand for 12h, and use it when no bacteria grow out. The activated Acetobacter xylinum suspension (about 1 × 10 7 cells / mL) were inoculated on the aliquoted fermentation medium in an amount of 5% (v / v), and cultured in a constant temperature and humidity chamber at 30° C. for 5 days.

[0044] Control group 2: Take glucose 10g, mannitol 5g, peptone 5g, yeast powder 5g, MgSO 4 ·7H 2 Add 1000mL of distilled water to fully dissolve O 1g, adjust the pH value to 6.8 with 0.1mol / L NaOH...

Embodiment 3

[0049] Take 15g of 30-day-expired pure milk, 0.3g of tea polyphenols, 5g of mannitol, 5g of peptone, 5g of yeast powder, and MgSO 4 ·7H 2 Add 1000mL of distilled water to fully dissolve O 1g, adjust the pH value to 6.8 with 0.1mol / L NaOH and 0.1mol / L HCl solution, put it in a high-temperature sterilization pot at 121°C for 20min, cool it until it is not hot, add 5mL of absolute ethanol, Continue cooling to room temperature to obtain a fermentation medium. Take 100mL of fermentation medium and put it into 250mL sterilized Erlenmeyer flasks, let it stand for 12h, and use it when no bacteria grow out. The activated Acetobacter xylinum suspension (about 1 × 10 7 cells / mL) were inoculated on the aliquoted fermentation medium in an amount of 5% (v / v), and cultured in a constant temperature and humidity chamber at 30° C. for 5 days.

[0050] Control group 3: take glucose 10g, mannitol 5g, peptone 5g, yeast powder 5g, MgSO 4 ·7H 2 Add 1000mL of distilled water to fully dissolve O...

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Abstract

The invention belongs to the technical field of functional materials, in particular to a bacterial cellulose facial mask with antioxidant ability and a preparation method thereof. The preparation method comprises the following steps: uniformly mixing and dissolving milk, tea polyphenols, mannitol, peptone, yeast powder and magnesium sulfate, adjusting pH value, sterilizing at high temperature, andadding appropriate amount of ethanol to prepare fermentation medium; selecting activated strains secreting bacterial cellulose to prepare bacterial seed liquid, and evenly inoculating the bacterial suspension with the concentration of about 1 *10<7> cells / mL into the fermentation culture medium, performing fermentation culture, purification of fermentation product, and cutting treatment to obtainbacterial cellulose mask with antioxidant ability. As that tea polyphenol is use as an antioxidant, the tea polyphenol is green and environmentally friendly, and at the same time, the facial mask substrate has certain whitening effect, and the in-situ static culture is adopt to prepare the antioxidant bacterial cellulose, which is simple in technology and easy to operate, and is suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of functional materials, and in particular relates to a bacterial cellulose mask with anti-oxidation ability and a preparation method thereof. Background technique [0002] With the improvement of living standards, people's pursuit of beauty is also constantly improving. As one of the most common and easy-to-carry skin care products, the demand for facial masks is increasing year by year. The facial masks sold on the market mainly include sheet, paste and mud, etc., and the mainstream sheet masks are mainly based on non-woven fabrics. Due to the low water holding capacity of non-woven fabrics, it is easy to cause waste of essence. In addition, non-woven fabrics have poor skin adhesion. Bacterial cellulose is a natural nano-polymer. It has become a new favorite in the research of mask substrates due to its good biodegradability, high biocompatibility and good water holding performance. During the cultivatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/99A61K8/49A61K8/9789A61Q19/00A61Q19/02A61Q19/08C12P19/04C12R1/01
CPCA61K8/498A61K8/99A61K2800/782A61K2800/85A61Q19/00A61Q19/02A61Q19/08C12P19/04
Inventor 杨仁党朱慧霞
Owner SOUTH CHINA UNIV OF TECH
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