Rapid detection kit of nitrite nitrogen, fabrication method of rapid detection kit and rapid detection method of nitrite nitrogen
A nitrite nitrogen and kit technology, which is applied in the direction of material analysis, measuring devices, instruments, etc. through observation of the influence on chemical indicators, can solve the problem of not providing an acidic buffer environment, the complicated operation process of the national standard method, and the reduction of stabilization time and other problems, to achieve the effect of low cost, stable detection reagents, and short stabilization time
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[0053] see figure 1 , the present invention provides a kind of preparation method of nitrite nitrogen rapid detection kit, comprising the following steps:
[0054] Step 1, prepare the color-developing water agent, and use an opaque eye drop bottle to pack the color-developing water agent; wherein, the step of preparing the color-developing water agent includes: mixing deionized water with concentrated hydrochloric acid to obtain the color-developing water agent Color agent.
[0055] Specifically, the eye drops bottle is a liquid container that can control the amount of drops that are dropped out by pinching or squeezing.
[0056] Specifically, the step 1 includes: adding a certain volume of deionized water to the clean container, then mixing a certain volume of concentrated hydrochloric acid, and then quantitatively dispensing it into an opaque eye drops bottle;
[0057] Specifically, in the color developing water agent, the mass ratio of concentrated hydrochloric acid to de...
Embodiment 1
[0134] Monitoring of nitrite nitrogen in water samples of an aquaculture fish shop in Pingdi Street, Longgang District, Shenzhen—0.65mg / L NO 2 - -N.
[0135] 1. The preparation method of the nitrite nitrogen quick test kit includes:
[0136] 1) Preparation of color developing water agent:
[0137] Dissolve 10ml of concentrated hydrochloric acid into 90ml of deionized water, mix evenly, use 10ml opaque eye droppers to dispense the mixed solution, and store in a cool place away from light.
[0138] 2) Preparation of chromogenic powder:
[0139] Weigh 2g of p-aminobenzenesulfonamide, 0.1g of N-(1-naphthyl)-ethylenediamine dihydrochloride, 4g of sodium chloride, and 4g of citric acid monohydrate, and pour them into a mortar and mix well . Dry in a vacuum oven for 12 hours, then pack into aluminum foil bags, the weight of each bag of medicine is 0.08g, and the size of the aluminum foil bags is 3.5cm×3.5cm. Finally, the aluminum foil bag is sealed with a vacuum sealer.
[014...
Embodiment 2
[0147] Shenzhen Changlong Technology Co., Ltd. Biological total nitrogen removal project pilot test (the main body of the water sample is wastewater from a domestic sewage plant in Gaoming, Foshan, Guangdong). The concentration of nitrite nitrogen—2.71mg / L NO 2 - -N.
[0148] 1. The preparation method of the nitrite nitrogen quick test kit includes:
[0149] 1) Preparation of color developing water agent:
[0150] Dissolve 20ml of concentrated hydrochloric acid into 180ml of deionized water, mix evenly, use 10ml opaque eye droppers to dispense the mixed solution, and store in a cool place away from light.
[0151] 2) Preparation of chromogenic powder:
[0152] Take by weighing 2.4g p-aminobenzenesulfonamide, 0.12g N-(1-naphthyl)-ethylenediamine dihydrochloride, 4.8g sodium chloride, 4.8g citric acid monohydrate four kinds of medicines respectively, pour into Bowl to mix. Place in a vacuum drying oven to dry for 12 hours, and pack into aluminum foil bags. The weight of ea...
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