Hydrogen peroxide biosensor based on sulfonated carbon nanotube composited with horseradish peroxidase, preparation and application thereof

A composite technology of horseradish peroxidase and nanotubes, which is applied in the direction of instruments, scientific instruments, and electrochemical variables of materials, can solve the problems of unfavorable non-pollution detection of the system to be tested, unfavorable hydrogen peroxide sensors, etc., and achieve good application Foreground, improvement of hydrophilicity, effect of good electron transport

Active Publication Date: 2019-01-15
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existence of electronic mediators is not conducive to the construction of hydrogen peroxide sensors, and is also not conducive to the pollution-free detection of the system to be tested.

Method used

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  • Hydrogen peroxide biosensor based on sulfonated carbon nanotube composited with horseradish peroxidase, preparation and application thereof
  • Hydrogen peroxide biosensor based on sulfonated carbon nanotube composited with horseradish peroxidase, preparation and application thereof

Examples

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Embodiment 1

[0044] A method for preparing a hydrogen peroxide biosensor based on sulfonated carbon nanotube composite horseradish peroxidase, comprising the following steps:

[0045] (1) The glassy carbon electrode with a diameter of 3 mm is sequentially filled with Al with a diameter of 0.3 μm and 0.05 μm 2 o 3 The powder is polished to a mirror surface, rinsed with distilled water, and then ultrasonically cleaned in absolute ethanol and distilled water for 1 min, taken out, rinsed with distilled water, and dried at room temperature to obtain a pretreated glassy carbon electrode;

[0046] (2) Place 100 mg of acidified carbon nanotubes (acidified carbon nanotubes) in 100 mL of distilled water for ultrasonic dispersion for 30 minutes to obtain a suspension of 1 mg / mL acidified carbon nanotubes; dissolve 72 mg of sodium nitrite in 40 mL of distilled water, Cool to 0-5°C to obtain an aqueous solution of sodium nitrite; then add 184mg of p-aminobenzenesulfonic acid and 2g of hydrochloric aci...

Embodiment 2

[0052] A method for preparing a hydrogen peroxide biosensor based on sulfonated carbon nanotube composite horseradish peroxidase, comprising the following steps:

[0053] (1) The glassy carbon electrode with a diameter of 3 mm is sequentially filled with Al with a diameter of 0.3 μm and 0.05 μm 2 o 3 The powder is polished to a mirror surface, rinsed with distilled water, and then ultrasonically cleaned in absolute ethanol and distilled water for 1 min, taken out, rinsed with distilled water, and dried at room temperature to obtain a pretreated glassy carbon electrode;

[0054] (2) Put 100 mg of acidified carbon nanotubes into 100 mL of distilled water for 30 minutes to obtain a suspension of 1 mg / mL acidified carbon nanotubes; dissolve 72 mg of sodium nitrite in 40 mL of distilled water and cool to 0-5°C , then 184mg p-aminobenzenesulfonic acid and 2g hydrochloric acid aqueous solution (1mol / L) were added into the sodium nitrite aqueous solution and mixed evenly, and ice-bat...

Embodiment 3

[0063] A method for preparing a hydrogen peroxide biosensor based on sulfonated carbon nanotube composite horseradish peroxidase, comprising the following steps:

[0064] (1) The glassy carbon electrode with a diameter of 3 mm is sequentially filled with Al with a diameter of 0.3 μm and 0.05 μm 2 o 3 The powder is polished to a mirror surface, rinsed with distilled water, and then ultrasonically cleaned in absolute ethanol and distilled water for 1 min, taken out, rinsed with distilled water, and dried at room temperature to obtain a pretreated glassy carbon electrode;

[0065] (2) Put 100 mg of acidified carbon nanotubes into 100 mL of distilled water for 30 minutes to obtain a suspension of 1 mg / mL acidified carbon nanotubes; dissolve 72 mg of sodium nitrite in 40 mL of distilled water and cool to 0-5°C , followed by adding 184mg p-aminobenzenesulfonic acid and 2g hydrochloric acid aqueous solution (1mol / L) into the sodium nitrite aqueous solution and uniformly mixing, ice ...

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Abstract

The invention belongs to the technical field of electrochemical biosensors, and discloses a hydrogen peroxide biosensor based on sulfonated carbon nanotube composited with horseradish peroxidase, preparation and application thereof. The biosensor is composed of a reference electrode, a counter electrode and a modified working electrode, the modified working electrode is composed of a working electrode and a substance recognition film solidified on the surface of the working electrode, wherein the substance recognition film is mainly composed of a sulfonated carbon nanotube dispersion, a horseradish peroxidase solution and a perfluorosulfonic acid resin solution, and formed on the surface of the working electrode. The invention also discloses a method for preparing a sensor. The preparationmethod of the invention is simple and low in cost; the obtained sensor has good selectivity, sensitivity and stability, the enzyme has a fixed quantity and stability, and has good electrocatalytic performance; the hydrogen peroxide biosensor has strong anti-jamming capability, a wide detection range and a low detection limit, and can detect the hydrogen peroxide accurately.

Description

technical field [0001] The invention belongs to the technical field of electrochemical biosensors, and in particular relates to an enzyme biosensor for detecting hydrogen peroxide based on sulfonated carbon nanotubes, a preparation method and application thereof. Background technique [0002] Hydrogen peroxide is an intermediate or product of many biochemical reactions in living organisms. Widely used in the food industry, mainly for disinfection, sterilization, disinfection, bread fermentation, etc., and can also be used as a processing aid in food additives. The national standard stipulates that processing aids should generally be removed from food and should not become a component of the final food, or only remain. In the process of food processing, the detection of residual hydrogen peroxide should be strictly controlled. At present, the methods for detecting hydrogen peroxide include: traditional methods for detecting hydrogen peroxide: titration, chemiluminescence, c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/30G01N27/48
CPCG01N27/308G01N27/327G01N27/48
Inventor 朴金花肖焱堃
Owner SOUTH CHINA UNIV OF TECH
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