Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Impurity analysis method for multiple vitamin preparations

A vitamin preparation and impurity analysis technology, which is applied to the analysis of materials, material separation, instruments, etc., can solve the problems of low ultraviolet absorption of impurities, large differences in vitamin content, and influence on accuracy, so as to improve separation and sensitivity, and ultraviolet absorption The effect of capacity enhancement and retention time increase

Active Publication Date: 2019-01-18
CHINESE MEDICINES GUANGZHOU
View PDF3 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Another prior art discloses the use of gas chromatography for detection, and the results show that the gasification temperature degrades dexpanthenol to generate pantothenic acid and 3-aminopropanol, which affects the accuracy of detection
3-Aminopropanol has strong polarity, almost no retention in reversed-phase chromatography, and low UV absorption
[0009] To sum up, the 13 kinds of vitamin preparations contain complex ingredients, and the content of each vitamin varies greatly. Among them, ingredients with large content and high polarity, such as vitamin C, niacinamide, dexpanthenol, vitamin B1, riboflavin sodium phosphate, etc. The detection of trace, highly polar and difficult-to-separate impurities (such as folic acid impurities) has a greater impact on the detection, and some impurities such as 3-aminopropanol have low UV absorption and low response values

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Impurity analysis method for multiple vitamin preparations
  • Impurity analysis method for multiple vitamin preparations
  • Impurity analysis method for multiple vitamin preparations

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Take the multivitamin preparation, dissolve it in water and dilute it to about 120 μg folic acid per 1 ml solution, as the test solution;

[0080] Take folic acid impurity A reference substance, folic acid impurity D reference substance, p-aminobenzoic acid reference substance and 3-aminopropanol reference substance, add 1ml 28mg / L sodium carbonate solution to dissolve, dilute with 10% methanol solution to every 1ml solution Containing 2.4 μg of folic acid impurity A, 2.4 μg of folic acid impurity D, 1.2 μg of p-aminobenzoic acid, and 3 μg of 3-aminopropanol, as the reference solution;

[0081] Take 25 mg of o-phthalaldehyde and 55 mg of 2-mercaptoethanol, add 0.5 ml of methanol to dissolve, and then add 5 ml of 0.4 mol / L boric acid buffer solution (adjust the pH value to 4.5 with phosphoric acid) to obtain a derivatization reagent;

[0082] Take 10 μL each of the above-mentioned test solution and reference solution, add 30 μL of derivatization reagents to carry out onl...

Embodiment 2

[0095] Take the multivitamin preparation, dissolve it in water and dilute it to about 120 μg folic acid per 1 ml solution, as the test solution;

[0096] Take folic acid impurity A reference substance, folic acid impurity D reference substance, p-aminobenzoic acid reference substance and 3-aminopropanol reference substance, add 1ml 28mg / L sodium carbonate solution to dissolve, dilute with 10% methanol solution to every 1ml solution Containing 2.4 μg of folic acid impurity A, 2.4 μg of folic acid impurity D, 1.2 μg of p-aminobenzoic acid, and 3 μg of 3-aminopropanol, as the reference solution;

[0097] Take 25 mg of o-phthalaldehyde and 55 mg of 2-mercaptoethanol, add 0.5 ml of methanol to dissolve, and then add 5 ml of 0.4 mol / L boric acid buffer solution (adjust the pH value to 4.5 with phosphoric acid) to obtain a derivatization reagent;

[0098] Take 10 μL each of the above-mentioned test solution and reference solution, respectively add 50 μL of derivatization reagent to c...

Embodiment 3

[0102] Take the multivitamin preparation, dissolve it in water and dilute it to about 120 μg folic acid per 1 ml solution, as the test solution;

[0103] Take folic acid impurity A reference substance, folic acid impurity D reference substance, p-aminobenzoic acid reference substance and 3-aminopropanol reference substance, add 1ml 28mg / L sodium carbonate solution to dissolve, dilute with 10% methanol solution to every 1ml solution Containing 2.4 μg of folic acid impurity A, 2.4 μg of folic acid impurity D, 1.2 μg of p-aminobenzoic acid, and 3 μg of 3-aminopropanol, as the reference solution;

[0104] Take 25 mg of o-phthalaldehyde and 55 mg of 2-mercaptoethanol, add 0.5 ml of methanol to dissolve, and then add 5 ml of 0.4 mol / L boric acid buffer solution (adjust the pH value to 4.5 with phosphoric acid) to obtain a derivatization reagent;

[0105] Take 10 μL each of the above-mentioned test solution and reference solution, add 30 μL of derivatization reagent for derivatizatio...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an impurity analysis method for multiple vitamin preparations. The method comprises the following steps of preparing a test solution of the vitamin preparations; dissolving o-phthalaldehyde and 2-mercaptoethanol based on a mass ratio of 1 to (2-5) in methanol, adding a boric acid buffer solution, and adjusting the pH value to 3-5 to obtain a derivatization reagent; and taking the test solution, adding the derivatization reagent to carry out online derivatization reaction, and injecting the derivatized test solution into a high performance liquid chromatograph, therebydetecting impurities in the test solution. According to the method, folic acid impurity A, folic acid impurity D, p-aminobenzoic acid and 3-aminopropanol in the vitamin preparations can be effectivelydetected; and the method is high in sensitivity and good in specificity.

Description

technical field [0001] The invention relates to the technical field of drug analysis, in particular to an impurity analysis method for multivitamin preparations. Background technique [0002] Vitamins are one of the essential nutrients for the human body, and can be divided into water-soluble vitamins and fat-soluble vitamins according to their solubility in aqueous solution. Water-soluble vitamins mainly include: thiamine (vitamin B1), riboflavin (vitamin B2), niacin (vitamin PP, niacin, niacinamide), pyridoxine (vitamin B6), cyanocobalamin (vitamin B12) , folic acid, pantothenic acid, biotin, ascorbic acid (vitamin C), etc., are easily soluble in water and can hardly be stored in the body. Fat-soluble vitamins mainly include vitamins A, D, E and K. As one of the essential nutrients for the human body, all vitamins are contained in a small amount in the body, but they play an important role in the metabolism, growth, and development of the body. The common characteristic...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 刘莉黄宏轩
Owner CHINESE MEDICINES GUANGZHOU
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com