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Lentinan capsule capable of efficiently amplifying NK cells in vivo, and preparation method thereof

A technology of lentinan and NK cells, which is applied in the field of medicine, can solve the problems of affecting the therapeutic effect of tumors, the uncertainty and unsafety of NK cell culture, etc., and achieve the effects of improving body immunity, increasing absorbability, and promoting proliferation

Active Publication Date: 2019-02-01
HUBEI CHUANGLI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above method can stimulate the efficient expansion of NK cells, the promotion of NK cells in vitro for anti-tumor involves animal serum with complex components. Mycoplasma and viruses may be introduced during the sampling process, causing uncertainty and unsafety for NK cell culture. , thus affecting the efficacy of tumor therapy

Method used

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  • Lentinan capsule capable of efficiently amplifying NK cells in vivo, and preparation method thereof
  • Lentinan capsule capable of efficiently amplifying NK cells in vivo, and preparation method thereof
  • Lentinan capsule capable of efficiently amplifying NK cells in vivo, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1. Lentinan capsule formula

[0035] Mushroom powder 45g, grass powder 50g, soybean powder 10g, tricalcium phosphate 3g, magnesium stearate 1g, xylitol 0.1g, sorbic acid 0.02g, sorbitol 6g.

[0036] 2. Preparation method

[0037] (1) Take the prepared shiitake mushroom powder, add it to 50g of water, soak for 3 hours, then extract it at a subcritical water temperature of 140-180°C for 5 minutes, and repeat the extraction twice;

[0038](2) Concentrate the extract in step (1) to a relative density of 1.15 g / cm at 60°C 3 Add 20ml of ethanol to precipitate, and centrifuge to obtain the precipitate; the precipitate is washed successively with 100g of absolute ethanol and 100g of acetone, and the washed precipitate is dissolved in 100g of distilled water, and passed through a hollow fiber membrane with a molecular weight cut-off of 1,000,000. The liquid is centrifuged, filtered, and the filtrate is taken for subsequent use;

[0039] (3) Take the prepared fluttering grass ...

Embodiment 2

[0045] 1. Lentinan capsule formula

[0046] Mushroom powder 65g, grass powder 60g, soybean powder 12.5g, tricalcium phosphate 4g, epoxy soybean oil 2g, D-xylose 0.175g, sorbic acid 0.035g, sorbitol 7.5g.

[0047] 2. Preparation method

[0048] (1) Take the prepared shiitake mushroom powder, add it to 97.5g of water, soak for 3.5 hours, and then extract for 6 minutes at a subcritical water temperature of 140-180°C, and repeat the extraction for 4 times;

[0049] (2) Concentrate the extract in step (1) to an extract with a relative density of 1.20g / cm3 at 60°C, add 40ml of ethanol to precipitate, and centrifuge to obtain a precipitate; Wash, dissolve the washed precipitate with 195g of distilled water, pass through a hollow fiber membrane with a molecular weight cut-off of 1 million, collect the permeate and centrifuge, filter, and take the filtrate for later use;

[0050] (3) Take the prepared fluttering grass powder, 195g of water, and 40g of ethanol and mix them into a dist...

Embodiment 3

[0056] 1. Lentinan capsule formula

[0057] Mushroom powder 80g, grass powder 75g, soybean powder 15g, tricalcium phosphate 5g, phosphite 3g, EDTA disodium salt 0.25g, sorbic acid 0.05g, sorbitol 9g.

[0058] 2. Preparation method

[0059] (1) Take the prepared shiitake mushroom powder, add it to 160g of water, soak for 4 hours, then leaching for 8 minutes at a subcritical water temperature of 140-180°C, and repeat the leaching for 3 times;

[0060] (2) Concentrate the extract in step (1) to an extract with a relative density of 1.25g / cm3 at 60°C, add 80ml of ethanol for precipitation, and centrifuge to obtain a precipitate; Wash, dissolve the washed precipitate with 320 g of distilled water, pass through a hollow fiber membrane with a molecular weight cut-off of 1 million, collect the permeate and centrifuge, filter, and take the filtrate for later use;

[0061] (3) Take the prepared flutter grass powder, 300g of water, and 45g of ethanol and mix them into a distiller, then...

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Abstract

The invention relates to the technical field of medicine, particularly to a lentinan capsule capable of efficiently amplifying NK cells in vivo, and a preparation method thereof, wherein the mushroompolysaccharide capsule comprises 45-80 parts of mushroom powder, 50-75 parts of Dichotomous Fimbristylis Herb powder, 10-15 parts of soybean powder, 3-5 parts of tricalcium phosphate, 1-3 parts of a stabilizer, 0.1-0.25 part of an antioxidant, 0.02-0.05 part of a preservative, and 6-9 parts of a moisturizer. According to the present invention, by combining the Dichotomous Fimbristylis Herb bioactive substance with effects of in vivo NK cell proliferation and differentiation promoting and immunity improving and the lentinan capable of enhancing the activity of NK cells, the obtained lentinan capsule has remarkable antitumor effect; the soybean power added to the formula can increase the absorption of the body to the bioactive ingredients; and the preparation method is simple, the extractedlentinan and the Dichotomous Fimbristylis Herb bioactive substance are high, almost no impurities and harmful substances exist, and the product has high safety and good treatment in tumor treatment.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a lentinan capsule for efficiently expanding NK cells in vivo and a preparation method thereof. Background technique [0002] Lentinan capsules are mainly composed of lentinan. Lentinus polysaccharide has immune enhancing effect. Although its mechanism does not directly kill tumor cells in the body, it can exert anti-tumor activity by enhancing the immune function of the body. In vivo, it can enhance the activity of NK cells in the spleen and peritoneal cavity, and its activity has a synergistic effect with interleukins or interferon inducers. [0003] Tumor immunotherapy is recognized as the fourth major tumor treatment method by domestic and foreign medical circles, among which the autologous immune cell (T cell, NK cell) treatment technology is the first batch of third-class medical technology allowed to be used clinically by the Ministry of Health. In recent years, natural...

Claims

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Application Information

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IPC IPC(8): C12N5/0783
CPCC12N5/0646C12N2501/90C12N2500/76
Inventor 张仁刘刘少敏谢华郭传功
Owner HUBEI CHUANGLI PHARMA
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