Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing dipeptidyl peptidase-IV inhibitory peptide derived from collagen of swim bladder of Nibea japonica

A technology of dipeptidyl peptidase and fish swim bladder collagen, which is applied in the field of protein extraction, can solve the problem of low resource utilization and achieve the effect of low dosage, good application prospect and remarkable physiological activity

Active Publication Date: 2019-02-01
ZHEJIANG OCEAN UNIV
View PDF13 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to overcome the problems of a large number of by-products and low resource utilization in the processing of Japanese croaker, the present invention provides a method for preparing dipeptidyl peptidase-IV inhibitory peptide derived from collagen in the swim bladder of Japanese croaker. The active peptide prepared by the invention has significant physiological activity and can be used in the fields of medicine, functional food, food, etc., so it has a good application prospect

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing dipeptidyl peptidase-IV inhibitory peptide derived from collagen of swim bladder of Nibea japonica
  • Method for preparing dipeptidyl peptidase-IV inhibitory peptide derived from collagen of swim bladder of Nibea japonica
  • Method for preparing dipeptidyl peptidase-IV inhibitory peptide derived from collagen of swim bladder of Nibea japonica

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Pretreatment of Japanese yellow croaker swim bladder:

[0032] (a) Exhausting the swim bladder, freeze-drying, and crushing to a particle size of 15 μm to obtain frozen swim bladder powder;

[0033] (b) adding the frozen swim bladder powder obtained in step (a) into 0.2mol / L temperature-controlled alkaline ionic liquid bis-N-(2-diethylamino)ethyl polyethylene glycol bis-imidazole hydroxide salt, First, at 22°C, the ultrasonic power density is 0.25w / cm 2 , ultrasonic treatment for 15min; then at 14°C, ultrasonic power density 1.0w / cm 2 , ultrasonic treatment for 6min; finally at 6°C, ultrasonic power density 2.2w / cm 2 , sonicated for 2 minutes; centrifuged and dried to obtain fish maw powder after removing impurities and defatting;

[0034] (2) extract collagen with 5 wt% acetic acid combined with pepsin, and centrifugally dry to obtain Japanese yellow mackerel swim bladder collagen;

[0035] (3) Use pH-responsive gel factor to modify microbial protease to enzymo...

Embodiment 2

[0038] (1) Pretreatment of Japanese yellow croaker swim bladder:

[0039] (a) Exhausting the swim bladder, freeze-drying, and pulverizing to a particle size of 5 μm to obtain frozen swim bladder powder;

[0040](b) adding the frozen swim bladder powder obtained in step (a) into 0.05mol / L temperature-controlled alkaline ionic liquid bis-N-(2-diethylamino)ethyl polyethylene glycol bis-imidazole hydroxide salt, First, at 20°C, the ultrasonic power density is 0.2w / cm 2 , ultrasonic treatment for 10min; then at 15°C, ultrasonic power density 0.7w / cm 2 , ultrasonic treatment for 5min; finally at 4°C, ultrasonic power density 2.5w / cm 2 , sonicated for 1 min; centrifuged and dried to obtain fish maw powder after removing impurities and defatting;

[0041] (2) extract collagen with 5 wt% acetic acid combined with pepsin, and centrifugally dry to obtain Japanese yellow mackerel swim bladder collagen;

[0042] (3) Utilize the pH response gel factor modified microbial protease to enzy...

Embodiment 3

[0045] (1) Pretreatment of Japanese yellow croaker swim bladder:

[0046] (a) Exhausting the swim bladder, freeze-drying, and crushing to a particle size of 20 μm to obtain frozen swim bladder powder;

[0047] (b) adding the frozen swim bladder powder obtained in step (a) into 0.5mol / L temperature-controlled alkaline ionic liquid bis-N-(2-diethylamino)ethyl polyethylene glycol bis-imidazole hydroxide salt, First, at 25°C, the ultrasonic power density is 0.35w / cm 2 , sonicate for 10min; then at 10°C, ultrasonic power density 1.5w / cm 2 , sonicate for 8min; finally at 8°C, ultrasonic power density 2.5w / cm 2 , sonicated for 1 min; centrifuged and dried to obtain fish maw powder after removing impurities and defatting;

[0048] (2) extract collagen with 5 wt% acetic acid combined with pepsin, and centrifugally dry to obtain Japanese yellow mackerel swim bladder collagen;

[0049] (3) Utilize pH responsive gel factor modified microbial protease to enzymolyze the Japanese yellowt...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of protein extraction. In order to solve the problem that a large amount of by-products are generated during processing of Nibea japonica and the resourceutilization rate is low, the invention provides a preparation method of dipeptidyl peptidase-IV inhibitory peptide derived from collagen of swim bladder of Nibea japonica. The method comprises the following steps: (1) pretreating swim bladder of Nibea japonica, then extracting collagen with 5 wt% acetic acid combined with pepsin, and carrying out centrifugal drying to obtain collagen of swim bladder of Nibea japonica; (2) carrying out enzymolysis on the collagen of swim bladder of Nibea japonica by the use of compound enzyme so as to obtain collagen peptide of swim bladder of Nibea japonica; and (3) screening a kit by combining the collagen peptide of swim bladder of Nibea japonica with dipeptidyl peptidase so as to obtain the dipeptidyl peptidase-IV inhibitory peptide. With the swim bladder of Nibea japonica as the main raw material and through an efficient technology, the dipeptidyl peptidase-IV inhibitory peptide is prepared. The inhibitory peptide has a good effect of inhibiting dipeptidyl peptidase-4, and has a good anti-hyperglycemic effect.

Description

technical field [0001] The invention relates to the technical field of protein extraction, in particular to a preparation method of dipeptidyl peptidase-IV inhibitory peptide derived from collagen in the swim bladder of Japanese yellow croaker. Background technique [0002] Diabetes has become the third metabolic disease that seriously threatens human health and life after malignant tumors and cardiovascular disease. It can cause various complications such as cerebrovascular disease, coronary heart disease, retinopathy, and even death. At present, the control and treatment of diabetes mainly rely on food control and drugs. Drugs for treating diabetes are mainly synthetic drugs, and they all have certain toxic and side effects. [0003] Dipeptidyl peptidase-4 (Dipeptidylpeptidase-IV, DPP-IV) in incretin drugs is a serine protease that can rapidly cleave and inactivate incretins, neuropeptides and cytokines. DPP-4 can specifically cut off the alanine or proline residue at th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 唐云平江晓霞丁国芳余方苗杨最素黄芳芳陈艳
Owner ZHEJIANG OCEAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com