A fermented carbonized tobacco leaf particle and its preparation method and application

A technology of tobacco leaves and granules, which is applied in the field of fermented carbonized tobacco granules and its preparation, can solve the problems of inability to add flavor to cigarettes, ineffective adsorption of harmful substances in smoke, poor coordination of cigarette aromas, etc., and achieve low cost and good business Application value, the effect of facilitating industrial production

Active Publication Date: 2021-01-29
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the disadvantages of the existing plant particles, such as insignificant adsorption effect on smoke harmful substances, inability to directly add aroma to cigarettes, or poor coordination with cigarette aroma, etc., the present invention provides a fermented carbonized tobacco leaf particle and its preparation method and application. By modifying the plant raw materials by using the fermenting and carbonization process of the tobacco Soil Dainella L4-6, it is possible to provide a product that can not only enhance the effect of cigarette harm reduction and tar reduction, reduce the irritation of cigarette smoke, but also enhance the effect of cigarette flavoring and enhance the quality of cigarettes. cigarette smoking quality

Method used

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  • A fermented carbonized tobacco leaf particle and its preparation method and application
  • A fermented carbonized tobacco leaf particle and its preparation method and application
  • A fermented carbonized tobacco leaf particle and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. Isolation, cultivation and identification of Tobacco Solanum Daiia L4-6

[0040] 1.1 Isolation of Tobacco Soil Daeella L4-6

[0041]Tobacco soil samples were taken from the tobacco planting base in Kunming, Yunnan, sealed in plastic bags, and stored at 4°C for later use. Accurately weigh 10g of soil sample, add 90mL of sterile water, shake on a shaker at 30°C and 180rpm for 30min; dilute the concentration to 10 with sterile water -1 、10 -2 、10 -3 、10 -4 、10 -5 Take 0.2mL of the five concentrations of bacterial solution and spread it on the LB plate medium, set up three parallel experiments for each concentration of the bacterial solution, cultivate it at 30°C for 48 hours, and pick it from the plate of each soil sample For different single colonies, after purification on LB plate medium, 20% of the bacterial volume was added to glycerol, and stored in a -80°C refrigerator for later use.

[0042] The isolated microorganisms were respectively inoculated in LB liq...

Embodiment 2

[0058] 1. Cultivation of Tobacco Soil D. L4-6

[0059] (1) Test tube slant culture

[0060] Slope preservation medium is used, the medium is R2A agar medium, and the formula of the medium is: 0.5g of glucose, 0.5g of yeast extract, 0.5g of peptone, 0.5g of acid hydrolyzed casein, 0.5g of soluble starch, and 0.3g of sodium pyruvate , dipotassium hydrogen phosphate 0.3g, magnesium sulfate 0.05g, agar 15g, distilled water to 1000mL, pH7.2. Sterilize the culture medium at 121°C for 25 minutes, place it on a slant, inoculate it with the strain L4-6 of Agrobacterium nicotianae, and cultivate it at 28°C for 1 week to obtain the strains in the test tube;

[0061] (2) Seed cultivation

[0062] Adopt seed medium, the formula of seed medium is: dextrin 120g, soybean powder 40g, yeast extract 2g, tryptophan 0.5g, β-alanine 5g, magnesium sulfate 0.5g, ammonium phosphate 0.2g, distilled water constant volume to 1000mL, pH7.2. Sterilize the culture medium at 121°C for 25 minutes, pick pa...

Embodiment 3

[0073] 1. The culture method of Tobacco Soil Dainella L4-6 is the same as in Example 2.

[0074] 2. Tobacco leaf fermentation

[0075] Weigh 600g of tobacco leaves pre-balanced to 12% moisture, spray 120mL of inoculum of Agrobacterium nicota L4-6, put the treated tobacco leaves in a constant temperature and humidity box at 22°C and 60% for fermentation for 72h.

[0076] 3. Carbonization of fermented tobacco leaves

[0077] Put the fermented tobacco leaves into the carbonization furnace, gradually raise the temperature of the furnace to 80°C, and keep it for 30 minutes. After the fermented tobacco leaves are dried, quickly raise the temperature to 130°C, and keep it for 3 hours. After checking the degree of carbonization, turn off the heat source, and take out the fermentation carbonization Tobacco samples are reserved.

[0078] 4. Preparation of Fermented Carbonized Tobacco Leaf Granules

[0079] Take the fermented carbonized tobacco leaves, check their moisture content, ma...

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Abstract

The invention provides a kind of fermented carbonized tobacco leaf particle and its preparation method and application, it is prepared by the method comprising the following steps: (1) the preparation of tobacco soil Dai's bacteria L4‑6 bacterial agent; (2) using tobacco soil Dai's Bacteria L4-6 bacteria agent ferments the tobacco leaves to obtain fermented tobacco leaves; (3) carbonizes the fermented tobacco leaves to obtain fermented carbonized tobacco leaves; (4) places the fermented carbonized tobacco leaf powder on a fluidized bed to dry, and sprays the binder , bond the powder to form granules, and continue drying to obtain fermented carbonized tobacco leaf granules. After the granules are fermented, they can reduce the irritation of the smoke, increase the richness of the cigarette aroma, and at the same time increase the sweetness of the smoke; after carbonization, they can significantly increase the adsorption specific surface area inside the tobacco leaves, and strengthen the granule’s effect on cigarette smoke. Adsorption of harmful substances in the air, and at the same time endow the plant particles with a special burnt aroma.

Description

technical field [0001] The invention belongs to the technical field of cigarette materials, and in particular relates to fermented carbonized tobacco leaf particles and a preparation method and application thereof. Background technique [0002] Cigarette aroma is the core content of cigarette quality, but with the development of low-coke cigarettes, the reduction of cigarette smoking taste and lack of aroma have become prominent problems in the development of tobacco. How to overcome the key technologies that affect the aroma and aroma of tobacco leaves has become the core of improving the competitiveness of cigarette production. Adding tobacco flavors and fragrances to shredded cigarettes is a conventional and necessary technical means to add aroma, but the space for improving the quality of cigarettes is relatively limited. It has become a new development trend to improve the aroma of cigarettes and reduce the irritation of cigarette smoke. Among them, adding some porous...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A24D3/14A24B15/20
CPCA24B15/20A24D3/14
Inventor 李源栋高莉杨洪明何靓焦俊苏勇夏建军
Owner CHINA TOBACCO YUNNAN IND
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