Preparation method of hydroxyapatite/modified polylactic acid composite microspheres

A technology of hydroxyapatite and composite microspheres, which is applied in pharmaceutical formulations, microcapsules, coatings, etc., can solve the problems of easy agglomeration of hydroxyapatite particles and uneven performance of composite materials, and achieves improved hydrophilicity, Improved biological activity and good interface compatibility

Inactive Publication Date: 2019-02-19
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the polylactic acid material lacks active functional groups that can interact with hydroxyapatite, the prepared hydroxyapatite / polylactic acid composites usually cause two-phase separation due to the incompatibility of the interface between the two materials. However, there are problems such as uneven performance of hydroxyapatite particles and easy agglomeration of hydroxyapatite particles, and there is no technology that can successfully prepare hydroxyapatite / polylactic acid composite materials into microspheres

Method used

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  • Preparation method of hydroxyapatite/modified polylactic acid composite microspheres
  • Preparation method of hydroxyapatite/modified polylactic acid composite microspheres

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Polylactic acid was dissolved in 1,4-dioxane solvent at 60 °C to prepare 20 g of a 1.0 wt% homogeneous solution, and a 1.2% mass percent ethylenediamine aqueous solution was added, and 40 g of glycerin, stirred evenly to form a stable emulsion. The emulsified system was placed in a refrigerator at -15°C for 4 h to solidify the polylactic acid droplets into microspheres. After filtering, washing and freeze-drying, the modified polylactic acid microspheres with amino active groups were obtained. The shape of the microspheres was The appearance and structure are as shown in the accompanying drawings of the description figure 1 shown. It can be seen from the figure that the obtained microspheres are round in shape, about 30 μm in size, and have a uniform and fine network nanofiber structure.

[0018] The concentration in 10 mL is 1.0 mol·L -1 Ca(NO 3 ) 2 20 mg of amino-modified polylactic acid nanofiber microspheres were put into the solution, and the mixed system of t...

Embodiment 2

[0020] Dissolve polylactic acid in 1,4-dioxane solvent at 70 °C to prepare 20 g of 1.5 wt% homogeneous solution, add ethylenediamine aqueous solution with a mass percentage of 1.5%, react for 30 min, and then add 60 g of glycerin, stirred evenly to form a stable emulsion. The emulsified system was placed in a refrigerator at -15°C for 4 h to solidify the polylactic acid droplets into microspheres. After filtering, washing and freeze-drying, the modified polylactic acid microspheres with amino active groups were obtained. Microspheres were observed by scanning electron microscope. The balls have a network of nanofiber structures.

[0021] The concentration in 10 mL is 1.5 mol·L -1 Ca(NO 3 ) 2 20 mg of amino-modified polylactic acid nanofiber microspheres were put into the solution, and the mixed system of the microspheres and the solution was placed in a constant temperature water bath shaker at 37 °C for oscillating adsorption for 4 days, and then filtered and separated. T...

Embodiment 3

[0023] Dissolve polylactic acid in 1,4-dioxane solvent at 60 °C to prepare 20 g of 0.5 wt% homogeneous solution, add ethylenediamine aqueous solution with a mass percentage of 1.0 %, react for 30 min, and then add 40 g of glycerin, stirred evenly to form a stable emulsion. The emulsified system was placed in a refrigerator at -15°C for 4 h to solidify the polylactic acid droplets into microspheres. After filtering, washing and freeze-drying, the modified polylactic acid microspheres with amino active groups were obtained. Microspheres were observed by scanning electron microscope. The balls have a network of nanofiber structures.

[0024] 0.5 mol·L in 10 mL -1 Ca(NO 3 ) 2 20 mg of amino-modified polylactic acid nanofiber microspheres were put into the solution, and the mixed system of the microspheres and the solution was placed in a constant temperature water bath shaker at 37 °C for oscillating adsorption for 5 days, and then filtered and separated. The concentration of ...

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Abstract

The invention discloses a preparation method of hydroxyapatite/modified polylactic acid composite microspheres. The preparation method is characterized in that ethylenediamine is used as a modifier toprepare amination-modified polylactic acid microspheres by the combination of aminolysis, emulsification and thermally induced phase separation; Ca(NO3)2 and K2HPO4 solutions and the amination-modified polylactic acid microspheres are used as inorganic calcium and phosphorus sources and an organic templating agent respectively to acquire amination-modified polylactic acid microspheres by in-situbiomimetic mineralization precipitation. The preparation method has the advantages of good environmental friendliness, good process simplicity, low cost and the like; amino groups in the modified polylactic acid provide great chelating action upon Ca2+ of hydroxyapatite, so that the modified polylactic acid and hydroxyapatite have good interfacial compatibility; hydroxyapatite crystal drops rarelyand can disperse evenly. Compared with polylactic acid microspheres, the composite microspheres herein can gain evidently improved bioactivity and bone-repairing performance, can effectively carry alendronate sodium drug and is an ideal bone-repairing material.

Description

technical field [0001] The invention relates to the preparation technology of bone repair biological materials, in particular to a preparation method of hydroxyapatite / modified polylactic acid composite microspheres. Background technique [0002] Due to its good biocompatibility and biodegradability, high strength, good plasticity, and easy molding and processing, polylactic acid material has become an ideal tissue engineering scaffold material, and has been widely used in the field of tissue engineering. Polylactic acid microspheres are ideal microcarriers for drugs and cells, and can be used for drug loading and tissue repair. The advantage of drug-loaded microspheres as tissue engineering scaffolds is that they can be directly implanted into the body through simple injection without complicated surgical treatment, and can also promote tissue repair by means of sustained and slow release of drugs at the lesion site. It has great application potential in the repair and tre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/32A61L27/50A61L27/58C08G63/91
CPCA61L27/32A61L27/50A61L27/58A61L2300/216A61L2300/622C08G63/912C08L67/04
Inventor 陈顺玉谢春玲肖秀峰
Owner FUJIAN NORMAL UNIV
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