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A device and method for reducing kasugamycin content in kasugamycin residue

A technology of kasugamycin and residue, which is applied in the field of reducing the content of kasugamycin residue in kasugamycin, can solve the problems of low combustion calorific value, increased enterprise cost, and high daily incineration cost, so as to improve biogas production efficiency, reduce treatment costs, The effect of reducing environmental pollution

Active Publication Date: 2021-01-05
陕西绿盾环境工程研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] (2) The water in the kasugamycin residue is not easy to separate, and most of the water exists in the cells of the mycelium of Streptomyces aureus, and ultracentrifugation pretreatment can only remove part of the extracellular water;
[0008] (3) Due to the high water content of kasugamycin residue, if it is directly incinerated without treatment, its treatment equipment includes incinerators, heat exchangers, tail gas treatment devices, and absorption towers. Low value will inevitably increase the cost of the enterprise, etc.

Method used

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  • A device and method for reducing kasugamycin content in kasugamycin residue
  • A device and method for reducing kasugamycin content in kasugamycin residue
  • A device and method for reducing kasugamycin content in kasugamycin residue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] In order to determine the impact of time and voltage on the kasugamycin content of kasugamycin residue, select 178 hours of fermentation liquid (mycelia scanning electron microscope see figure 2 ) The acidified acidified solution is filtered through a ceramic membrane, the selected voltage is 30, 50, 70, 90, 110, 130, 170 volts of direct current voltage, the electrophoresis liquid in the electrophoresis tank is PBS buffer (pH=7.2), electrophoresis The time is 90min, and the porous supports are agarose gel, polyacrylamide gel and pectin. The specific results are shown in Table 1.1.

[0061] Table 1.1 The effect of different voltages on the removal of Kasugamycin residues

[0062]

[0063] It can be seen from Table 1.1 that as the voltage continues to increase, the removal rate of kasugamycin of each support residue is constantly increasing, between 30-100 volts, the greater the increase, and after 110 volts, the upward trend Not obvious; between 30-50 volts of DC vo...

Embodiment 2

[0065] In order to determine the impact of time on the kasugamycin content of the kasugamycin residue, select the electrophoresis time to be 30, 45, 60, 75, 90, 105 and 120min, DC voltage 110 volts, the electrophoresis liquid of the electrophoresis tank is PBS buffer (pH =7.2), the porous supports are agarose gel, polyacrylamide gel and pectin, the specific results are shown in Table 2.1.

[0066] Table 2.1 Effect of different electrophoresis time on the removal effect of kasugamycin residues

[0067]

[0068] It can be seen from Table 2.1 that between the electrophoresis time of 30-90, as the electrophoresis time prolongs, the removal efficiency of kasugamycin of the residue increases with the prolongation of time, and the removal rate difference between the three supports is compared in pairs Not significant (p>0.05), between 90-120, the removal rate of kasugamycin under each substrate is decreased, because the migration rate of positive and negative ions charged by kasug...

Embodiment 3

[0070] In order to determine whether the kasugamycin content of the kasugamycin residue affects the removal rate of the residue kasugamycin, the kasugamycin residue in the ceramic membrane filtration section is selected, diluted 2 times with the residue quality, stirred evenly, the electrophoresis voltage is 110 volts, and the electrophoresis The time is 90min, and the electrophoresis fluid in the electrophoresis tank is PBS buffer (pH=7.2). The specific results are shown in Table 2.1.

[0071] Table 3.1 Effect of kasugamycin content in kasugamycin residue on the removal rate of kasugamycin in residue

[0072]

[0073] It can be seen from Table 3.1 that when the kasugamycin residue is diluted in a ratio of 0-2 4 Between dilutions, the removal rate of kasugamycin under each support increases with the increase of the dilution factor, this is because kasugamycin hydrochloride is in the certain situation of each support space volume, and kasugamycin hydrochloride and The resis...

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Abstract

The invention relates to a device and method for decreasing the kasugamycin content in kasugamycin residue. A direct current electrophoresis and macromolecular glue separation technology is adopted, as for the adopted device, macromolecular glue is placed in a general electrophoresis device, a square hole is dug in the macromolecular glue, the macromolecular glue is arranged at the bottom layer ofthe hole to serve as an underskirt, PBS, a 0.8% sodium chloride solution and a Tris-HCl buffer solution serve as an electrolyte, and the kasugamycin residue is put into the square hole of the macromolecular glue or is directly subjected to electrophoresis. According to the device, kasugamycin in the kasugamycin residue can be well removed, thus the kasugamycin can be subjected to better resourceutilization, in addition, the macromolecular glue is a biodegradable material, the kasugamycin in the residue enters the macromolecular glue through electrophoresis, and the kasugamycin entering the macromolecular glue can be reutilized through the separation technology, wherein the macromolecular glue with the kasugamycin being removed can be recycled.

Description

technical field [0001] The invention belongs to the technical field of removing kasugamycin in the fermentation residue of Streptomyces aureus, and in particular relates to a method for reducing the kasugamycin content of the kasugamycin residue. Background technique [0002] Kasugamycin, also known as Kasugamycin, is a microbial-derived agricultural fungicide for the prevention and treatment of crop diseases. Its chemical name is (5-amino-2-methyl-6-(2,3, 4,5,6-Hydroxycyclohexyloxy)tetrahydropyran-3-yl)amino-α-iminoacetic acid, formula C 14 h 25 N 3 o 9 , molecular weight 379.4. The pure product of kasugamycin is white crystal; the hydrochloride is white needle-like or flaky crystal, the melting point of pure product: 236-239°C decomposes, the melting point of hydrochloride: 202-204°C (decomposition), easily soluble in water, in Dissolve 12.5% ​​(W / V) in water at 25°C, insoluble in methanol, ethanol, acetone, benzene and other organic solvents. Its structural formula ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/447G01N30/02
CPCG01N27/447G01N30/02
Inventor 潘忠成邓钊张楠赵涵珺李丽陈豪翁婧李向荣高波李蒲民
Owner 陕西绿盾环境工程研究院有限公司
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