Preparation method of thais clayigera antihypertensive peptide

Abstract

The invention provides a preparation method of a thais clayigera antihypertensive peptide and belongs to the field of biological technology. The method comprises providing a thais clayigera slurry which is obtained by impurity-cleaning and homogenizing thais clayigera; providing at least one protease to enzymolyze the slurry to obtain an enzymolyzed solution; extracting the enzymolyzed solution through ultrasonic-assisted extraction; performing debittering and purifying treatment on the enzymolyzed solution, and performing drying treatment on the thais clayigera antihypertensive peptide. The molecular weight of the thais clayigera antihypertensive peptide is 250-2500 Da. The invention also provides application of the thais clayigera antihypertensive peptide to the field of foods, healthcare products and medicines. The preparation method of the thais clayigera antihypertensive peptide is mild in condition, high in protein hydrolysis rate as well as yield and purity of products and few in enzymolysis dead angle; the prepared thais clayigera antihypertensive peptide is good in antihypertensive effects, high in inhibiting effects on ACE (angiotensin converting enzyme), excellent in sensual experience and high in stability, and can be applied as a functional factor to the fields of food, healthcare products and medicines, thereby increasing the value added of thais clayigera.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method of snail snail antihypertensive peptide. Background technique [0002] Lychee snails are commonly known as spicy glass snails, spicy snails, gastropods, neogastropods, and snails. The shells are small, spindle-shaped, hard and thick, and they are broad-temperate benthic molluscs. It is distributed in the coastal areas of China and is one of the most common snails in lithofacies intertidal zone. It is an important economic shellfish in Zhoushan Islands. Lychee snails have the functions of clearing heat and disinfecting, softening hard masses and resolving stagnation, and lowering blood pressure. According to reports, the protein content of snails accounts for 19% of its wet weight, and the amino acid content per 100g of soft body dry product reaches 68g, of which lysine, aspartic acid, arginine and other contents are all higher. [0003] Hypertension...

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Problems solved by technology

[0006] Existing technologies such as the Chinese invention patent with the authorized announcement number CN10398037B disclose a large yellow croaker swim bladder antihypertensive peptide and its preparation method and use. The hydrolyzate is purified by centrifugation, ultrafiltration and chromatography to obtain the antihypertensive peptide. Although the above method uses protease to prepare the antihypertensive peptide, there are few wastes, it belongs to a clean process, and the product safety is high, but the separation technology is mainly resin chromatography. , Gel column chromatography, from the perspective of product development, the development cost is too high, which is not conducive to industrial production

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