Biosynthesis preparation method for ergothioneine, and fermentation culture medium

A fermentation medium and ergothioneine technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of difficult purification treatment, increased fermentation by-products, low fermentation yield, etc.
CN109439701AActive Publication Date: 2019-03-08BLOOMAGE BIOTECHNOLOGY CORP LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
BLOOMAGE BIOTECHNOLOGY CORP LTD
Publication Date
2019-03-08

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Abstract

The invention relates to a biosynthesis preparation method for ergothioneine, and a fermentation culture medium. The method comprises the following steps of S1: inoculating Hericium erinaceus myceliumslant cultures to a liquid seed culture medium to be cultured to obtain seed liquid; S2: inoculating the obtained seed liquid to the fermentation culture medium to be fermented, adding precusor substances for culturing, and fermenting to a fermentation destination through pH (Potential of Hydrogen) judging a fermentation liquor; S3: after fermentation finishes, adding enzyme, carrying out enzymolysis to the destination, raising the temperature to kill enzyme, and lixiviating the ergothioneine in the cell of the mycelium to the fermentation liquor out of the cell. By use of the method disclosed by the invention, the yield of the ergothioneine can be improved, the fermentation destination can be quickly and simply judged, fermentation byproducts are reduced, the enzyme is used for breakingmycelium cells, and ergothioneine is safe to be lixiviated.
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Description

technical field

[0001] The invention relates to a method for biosynthesizing ergothioneine by using Hericium erinaceus mycelium and a fermentation medium used therefor, belonging to the field of microbial fermentation engineering. Background technique

[0002] Ergothioneine (EGT), scientifically named as 2-mercapto-L-histidine trimethyl inner salt, contains imidazole-2-thione group shown in the following general formula (1) in its molecular structure. Ergothioneine is a natural amino acid that exists in many animals and plants and is rich in content. It cannot be synthesized by the animal body itself and can only be ingested from food. It is a rare amino acid.

[0003]

[0004] Studies have shown that ergothioneine has strong antioxidant effects and can protect cells from cell damage or even death caused by ultraviolet rays and gamma rays. Therefore, it has broad application prospects in pharmaceutical, food and cosmetic industries. However, the content of ergothioneine ...

Claims

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