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Method for preparing PHA (polyhydroxyalkanoate)

A technology of polyhydroxyalkanoic acid ester and hydroxybutyric acid, which is applied in the downstream post-treatment field of bioengineering, can solve the problems of large amount of surfactant added, no consideration of industrialization enlargement, increased cost, etc., and achieves low price and reduces the difficulty of wastewater treatment. , Extract the effect of simple equipment

Pending Publication Date: 2019-03-22
BLUEPHA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has mild reaction conditions and low cost, but consumes a lot of alkali, adds a large amount of surfactant, and needs to add alkaline protease, which increases the cost additionally. It is only a laboratory process and does not consider industrial scale-up.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1: Extract PHA from Halomonas fermentation broth under laboratory conditions

[0044] 1. Halomonas halomonas TD01 [preservation number: CGMCC No.4353, has been authorized in the patent application (patent announcement number: CN102120973B)] uses glucose as the carbon source to ferment and produce PHB, ferment for 48 hours, and the biomass is 83g / L, PHB content 80%.

[0045] Fermentation medium:

[0046] Sodium chloride 40g / L, glucose 30g / L, corn steep liquor powder 10g / L, urea 2g / L, magnesium sulfate 0.2g / L, potassium dihydrogen phosphate 4.2g / L, trace element mother solution I 10mL / L, trace element Mother liquor II 1mL / L.

[0047] 1L trace element mother liquor I: Mix 5g ferric ammonium citrate and 2g calcium chloride dihydrate with 0.5mol / L hydrochloric acid aqueous solution, and make up to 1 liter with 0.5mol / L hydrochloric acid aqueous solution after complete dissolution.

[0048] 1L trace element mother liquor II: Zinc sulfate heptahydrate 100mg, mang...

Embodiment 2

[0076] Example 2: Extract PHA from Halomonas fermentation broth under pilot test conditions

[0077] Fermentation method: use Halomonas halomonas TD40 as the fermentation strain, and use glucose and γ-butyrolactone as the carbon source to ferment P3HB4HB, 1m 3 The fermentation system of the fermenter is 700L, the fermentation time is 36h, the biomass is 89g / L, and the P3HB4HB content is 65%.

[0078] Fermentation medium:

[0079] Sodium chloride 30g / L, glucose 30g / L, corn slurry 50mL / L, urea 2g / L, magnesium sulfate 0.8g / L, potassium dihydrogen phosphate 4g / L, trace element mother liquor I 10mL / L, trace element mother liquor II 10mL / L.

[0080] The preparation method of trace element mother liquor I and II is the same as that of the 7.5L fermentation system in Example 1.

[0081] Fermentation culture conditions:

[0082] The secondary seed liquid was inserted into the above-mentioned optimized fermentation medium according to the inoculum amount of 10%, and the 1000L fermen...

Embodiment 3

[0096] Embodiment 3: Extract PHA from Halomonas fermentation broth under industrialized conditions

[0097] Fermentation method: use Halomonas halomonas TD40 as the fermentation strain, and use glucose and γ-butyrolactone as the carbon source to ferment P3HB4HB, 5m 3 Fermentation tank fermentation system is 4m 3 , fermentation time 36h, biomass 99g / L, P3HB4HB content 65%.

[0098] Fermentation medium:

[0099] Sodium chloride 30g / L, glucose 30g / L, corn slurry 30mL / L, urea 2g / L, magnesium sulfate 1.2g / L, potassium dihydrogen phosphate 4g / L, trace element mother liquor I 10mL / L, trace element mother liquor II 10mL / L.

[0100] The preparation method of trace element mother liquor I and II is the same as that of the 7.5L fermentation system in Example 1.

[0101] Fermentation culture conditions:

[0102] Insert the secondary seed liquid into the above-mentioned optimized fermentation medium according to the inoculum amount of 10%, 5m 3 The initial filling liquid of the ferme...

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Abstract

The invention provides a method for preparing PHA (polyhydroxyalkanoate). The method comprises the following steps of (1) preparing halophilic bacterium fermentation liquid; performing solid-liquid separation to obtain concentration bacterium liquid; (2) washing the concentration bacterium liquid obtained in step (1); (3) performing cell wall breaking cracking on the washed bacterium liquid obtained in step (2) so as to obtain wall breaking liquid; (4) removing non-PHA substances in the wall breaking liquid obtained in step (3); collecting the PHA; (5) purifying the PHA; (6) drying the PHA. The method has the advantages that the process is mild; the cost is low; the requirement on the equipment is low; the large-scale industrialized production can be realized.

Description

technical field [0001] The invention belongs to the downstream post-treatment field of bioengineering, and in particular relates to a method for preparing polyhydroxyalkanoate (PHA). Background technique [0002] Polyhydroxyalkanoates (PHA for short) is a kind of storage granular matter synthesized by microorganisms under the condition of other nutrient limitation and carbon source excess, and it is a kind of biopolymer polyester compound. PHA has the characteristics of ordinary thermoplastics, but it is different from traditional petrochemical plastics. PHA has good biocompatibility, degradability, piezoelectricity and optical activity, making it widely used in industry, agriculture, medicine and health, food, etc. The field has broad application prospects. In the field of degradable agricultural mulch film and degradable plastic products and medical fields, PHA, as the best biodegradable material at present, has shown great potential. [0003] The traditional PHA producti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/62C08G63/90C08G63/06C12R1/01
CPCC08G63/06C08G63/90C12P7/625
Inventor 叶健文汪东升陈风义
Owner BLUEPHA CO LTD
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