Method for in-situ purification on aquaculture waste water by biochar reinforced bacterium and algae immobilized preparation

A technology of aquaculture and biochar, applied in biological water/sewage treatment, chemical instruments and methods, animal husbandry wastewater treatment, etc., to achieve the effect of improving stability, simple method, and promoting degradation efficiency

Active Publication Date: 2019-03-29
XIAMEN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, while using biochar powder to concentrate pollutants, improve the mass transfer energy of bacteria and algae-immobilized rubber balls, promote the contact between microorganisms and pollutants, improve the degradation efficiency of pollutants, and realize the in-situ purification of aquaculture wastewater. report

Method used

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  • Method for in-situ purification on aquaculture waste water by biochar reinforced bacterium and algae immobilized preparation
  • Method for in-situ purification on aquaculture waste water by biochar reinforced bacterium and algae immobilized preparation

Examples

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Embodiment 1

[0042] Example 1: Optimization of fixed conditions, using orthogonal experiments to determine the optimal ratio between bait algae, probiotics, biochar, sodium alginate and calcium chloride, so as to achieve better purification of aquaculture wastewater. The optimal preparation scheme is that the mass fraction of biochar powder is 0.8%, the concentration of sodium alginate is 2.5%, and the inoculation amount of algae is 2.18×10 7 cells, the inoculum size is 5.67×10 7 cells, the concentration of calcium chloride was 1.2%. It is verified by experiments that the mixed bacteria and algae solution has a better effect on treating aquaculture wastewater than a single strain of bacteria and algae solution. The removal efficiency of organic matter, ammoniacal nitrogen, nitrite nitrogen, nitrate nitrogen and dissolved phosphorus are 64.37%, 91.13%, 100%, 97.92%, 100%, respectively.

[0043] For the production of immobilized rubber balls, the probiotics were cultured for 40 hours, cent...

Embodiment 2

[0048] The sodium alginate solution with a concentration of 0.8% is heated, melted and cooled, and an appropriate amount of bait algae is added (the inoculum size of the algae is 2×10 5 cells), aquaculture probiotics (the inoculation amount of the bacteria is 5×10 7 cells) and biochar powder (mass fraction is 0.01%) were mixed evenly to form a mixed gel solution; the mass fraction was 0.6% calcium chloride solution for cross-linking to obtain immobilized beads; rinsed with normal saline and loaded Put it into a nylon mesh bag with holes, and immerse in the in-situ purification of aquaculture wastewater. Its removal efficiency of organic matter, ammonia nitrogen, nitrite nitrogen, nitrate nitrogen and dissolved phosphorus is 62.21%, 90.13*%, 99.21*%, 91.31%, 95.45%, respectively.

[0049] It is verified by experiments that this embodiment can greatly improve the efficiency of removing organic matter, ammoniacal nitrogen, nitrite nitrogen, nitrate nitrogen and dissolved phospho...

Embodiment 3

[0051] The difference from Example 2 is that the mass fraction of biochar powder is 1.8%, and the inoculation amount of bait algae is 2×10 8 cells, the inoculation volume of aquaculture probiotics is 5×10 8 cells. Through experimental verification, it removes 60.67%, 92.8*4%, 98.23*%, 93.86%, 98.21% of organic matter.

[0052] This embodiment can greatly improve the efficiency of removing organic matter, ammonia nitrogen, nitrite nitrogen, nitrate nitrogen and dissolved phosphorus, and has a good effect on treating aquaculture wastewater.

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Abstract

The invention discloses a method for in-situ purification on aquaculture waste water by a biochar reinforced bacterium and algae immobilized preparation. Sodium alginate and calcium chloride solutionare used as crosslinking agents; immobilized gel small balls formed by bait algae, aquaculture probiotics and biochar powder are embedded; the small balls are charged into a nylon wire net and are then soaked into the in-situ purification culture waste water. The specific surface area of the prepared immobilized small balls is improved and is large; the mass transfer performance is improved; the tolerance of microbes on the outside toxicity is enhanced; the degradation capability on contaminants is improved; the water purification goal is achieved.

Description

technical field [0001] The invention belongs to the field of wastewater treatment, and in particular relates to a system for immobilizing bacteria and algae, in particular to a method for in-situ purification of aquaculture wastewater with a biochar-enhanced immobilized preparation of bacteria and algae. Background technique [0002] In recent years, the problem of water pollution caused by aquaculture has attracted much attention. With the change of agricultural production structure in my country, the aquaculture industry has developed rapidly. The large amount of aquaculture wastewater produced will pose a serious threat to the surrounding natural water bodies and the surrounding environment, and will cause serious deterioration of water quality, increase of aquaculture viruses, and damage to the ecological balance of water bodies. Aquaculture wastewater is characterized by high content of organic matter and nitrogen and phosphorus, manifested as: severe eutrophication of w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/32C02F3/34C02F103/20
CPCC02F3/322C02F3/34C02F2103/20
Inventor 吴义诚吴洪杰毛睿韬
Owner XIAMEN UNIV OF TECH
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