Method for in-situ purification on aquaculture waste water by biochar reinforced bacterium and algae immobilized preparation
A technology of aquaculture and biochar, applied in biological water/sewage treatment, chemical instruments and methods, animal husbandry wastewater treatment, etc., to achieve the effect of improving stability, simple method, and promoting degradation efficiency
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Embodiment 1
[0042] Example 1: Optimization of fixed conditions, using orthogonal experiments to determine the optimal ratio between bait algae, probiotics, biochar, sodium alginate and calcium chloride, so as to achieve better purification of aquaculture wastewater. The optimal preparation scheme is that the mass fraction of biochar powder is 0.8%, the concentration of sodium alginate is 2.5%, and the inoculation amount of algae is 2.18×10 7 cells, the inoculum size is 5.67×10 7 cells, the concentration of calcium chloride was 1.2%. It is verified by experiments that the mixed bacteria and algae solution has a better effect on treating aquaculture wastewater than a single strain of bacteria and algae solution. The removal efficiency of organic matter, ammoniacal nitrogen, nitrite nitrogen, nitrate nitrogen and dissolved phosphorus are 64.37%, 91.13%, 100%, 97.92%, 100%, respectively.
[0043] For the production of immobilized rubber balls, the probiotics were cultured for 40 hours, cent...
Embodiment 2
[0048] The sodium alginate solution with a concentration of 0.8% is heated, melted and cooled, and an appropriate amount of bait algae is added (the inoculum size of the algae is 2×10 5 cells), aquaculture probiotics (the inoculation amount of the bacteria is 5×10 7 cells) and biochar powder (mass fraction is 0.01%) were mixed evenly to form a mixed gel solution; the mass fraction was 0.6% calcium chloride solution for cross-linking to obtain immobilized beads; rinsed with normal saline and loaded Put it into a nylon mesh bag with holes, and immerse in the in-situ purification of aquaculture wastewater. Its removal efficiency of organic matter, ammonia nitrogen, nitrite nitrogen, nitrate nitrogen and dissolved phosphorus is 62.21%, 90.13*%, 99.21*%, 91.31%, 95.45%, respectively.
[0049] It is verified by experiments that this embodiment can greatly improve the efficiency of removing organic matter, ammoniacal nitrogen, nitrite nitrogen, nitrate nitrogen and dissolved phospho...
Embodiment 3
[0051] The difference from Example 2 is that the mass fraction of biochar powder is 1.8%, and the inoculation amount of bait algae is 2×10 8 cells, the inoculation volume of aquaculture probiotics is 5×10 8 cells. Through experimental verification, it removes 60.67%, 92.8*4%, 98.23*%, 93.86%, 98.21% of organic matter.
[0052] This embodiment can greatly improve the efficiency of removing organic matter, ammonia nitrogen, nitrite nitrogen, nitrate nitrogen and dissolved phosphorus, and has a good effect on treating aquaculture wastewater.
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