Mixed feed additive for suckling piglets and preparation method thereof
A technology for feed additives and suckling piglets, applied in animal feed, animal feed, additional food elements, etc., can solve food safety problems, high diarrhea rate, low feed digestibility, etc., and achieve a significant effect on food safety
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[0052] Below in conjunction with accompanying drawing, preparation method of the present invention is described in detail;
[0053] like figure 1 Shown, the preparation method of the hybrid suckling piglet feed additive that the embodiment of the present invention provides:
[0054] S101: powdering Bacillus subtilis, Clostridium butyricum, Saccharomyces cerevisiae and xylitolase, and mixing them uniformly to obtain a mixed feed additive for suckling piglets;
[0055] S102: Determining the moisture content of the mixed feed additive for suckling piglets; sanitation testing;
[0056] S103: Inspection of mixed feed additives for suckling piglets: ex-factory inspection, type inspection, re-inspection;
[0057] S104: Label, package, transport and store the mixed feed additive for suckling piglets.
[0058] In step S101, the prepared powder provided by the embodiment of the present invention has uniform color and luster, no agglomeration, no moldy deterioration and peculiar smell...
Embodiment 1
[0081] Example 1; Bacillus subtilis
[0082] 1 Sample preparation, initial suspension and ten-fold dilution
[0083] Viable count detection of spores (Bacillus licheniformis and Bacillus subtilis)
[0084] Weigh 25g (ml) of the sample by aseptic operation, add it into a conical flask containing 225mL 0.85% sterilized physiological saline and glass beads, shake and homogenize for 30min, and make an initial suspension of 1:10. Draw 1mL of the initial suspension of 1:10, add 9L of 0.85% sterile saline, and mix well to make a 1:100 dilution. According to the bacterial content of the sample, further ten-fold serial dilutions were made.
[0085] 2 Inoculation and cultivation
[0086] Select 3 appropriate dilutions, use a sterile pipette to draw 0.1mL of the dilution respectively, inoculate them on the nutrient agar plate, do two parallels, use a coating stick to carefully and quickly spread the inoculum on the agar surface plate, and apply The cloth stick must not touch the edge...
Embodiment 2
[0099] Example 2; Clostridium butyricum
[0100] 1 Determination of the number of viable bacteria
[0101] 1.1 Reagents and solutions
[0102] a) Diluent (physiological saline dilution): Accurately weigh 8.5g of sodium chloride in a 1000ml volumetric flask, distill to volume, and sterilize.
[0103] b) RCM medium:
[0104] Peptone 10g, beef powder 10g, yeast powder 3g, glucose 5g, soluble starch 1g, sodium chloride 5g, sodium acetate 3g, L-cysteine hydrochloride 0.5g, agar 8-10g, distilled water 1000mL, final pH6. 8±0.1 (25°C), sterilized at 121°C for 30min.
[0105] 1.2 Instruments and equipment
[0106] This test uses general laboratory equipment: super bench, shaker: rotary 200r / min, constant temperature incubator, sterilizer, glass apparatus, vortex mixer for sample processing.
[0107] a) Weigh 25g (accurate to 0.01) of the sample, place it in a conical flask with glass beads and 225ml of diluent, shake it fully on a rotary shaker for 30 minutes (200r / min), and pre...
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