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Multifunctional rahnella aquatilis MEM40 and application thereof

A multifunctional technology of Lahenia water, applied in the field of microorganisms, can solve the problems of different nutritional requirements of strains, low soil survival rate, single function of strains, etc., and achieves reduction of fertilizer use, cultivation conditions and methods are simple and easy. good effect of promoting growth

Active Publication Date: 2019-04-05
湖北楚峰源种植有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are a large number of microorganisms in the soil, plant rhizosphere or plant tissue, among which the strains that have the functions of disease prevention, growth promotion or improvement of plant resistance have broad application prospects in improving fertilizer utilization and reducing the use of chemical pesticides, and have gradually become Research and development hot spot, but according to the current reported literature, most of the strains currently developed and applied have single functions, and the soil survival rate is not high. However, complex bacteria have antagonism among microorganisms, and the nutritional requirements of different strains are not the same, which causes a lot of inconvenience to practical application.

Method used

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  • Multifunctional rahnella aquatilis MEM40 and application thereof
  • Multifunctional rahnella aquatilis MEM40 and application thereof
  • Multifunctional rahnella aquatilis MEM40 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Screening and identification of MEM40

[0039] 1. It is separated from the soil by the enrichment culture method, as follows: 10g soil sample is collected from a phosphate chemical factory in Hubei, added to the sterilized PKO medium for enrichment culture, and then spread on the PKO plate, and the plate is picked Colonies with larger upper phospholytic circles were separated and purified several times to obtain the bacterial strain of the present invention, which was designated as MEM40.

[0040] 2. Observe the morphological characteristics of the strain, as follows: inoculate the purified bacteria on LB medium for overnight activation, streak on LB solid medium, culture at 30°C for 24 hours, observe the surface morphology of the colony, and observe the microscopic appearance of the strain by Gram staining. form.

[0041] 3. Identification of the physiological and biochemical properties of the strain: Physiological and biochemical analysis including carbon source util...

Embodiment 2

[0054] Experiment on the Inorganic Phosphorus-decomposing Effect of Lahnella water MEM40

[0055] The overnight activated MEM40 bacterial solution was inoculated into 200 mL of PKO medium, 30 ° C, 150 rpm constant temperature shaking culture, sampling 4 mL at intervals, and centrifuged at 10000 rpm for 8 min. According to the national standard method (GB 11893-89-molybdenum antimony anti-colorimetric method), the soluble phosphorus content was measured, and the culture medium not inoculated with the bacterial solution was used as the control. The results of the phosphorus-dissolving ability of MEM40 are shown in image 3 .

[0056] It can be seen from the figure that at 0-8h, MEM40 is in the adaptation period, and the amount of dissolved phosphorus is low. After 12h, the strain is in the logarithmic growth phase, and the ability to dissolve insoluble inorganic phosphorus gradually increases. At 32h, MEM40 dissolves calcium phosphate and magnesium phosphate. At 24 hours, the a...

Embodiment 3

[0058] Experiment on the Effect of Decomposing Organic Phosphorus by Lahnella hydrous MEM40

[0059] Spot the overnight activated MEM40 bacterial solution on a soybean lecithin plate, incubate it upside down at a constant temperature of 28°C for 3 days, and observe whether there are obvious phospholytic circles around the colonies.

[0060] Inoculate overnight activated MEM40 into conical flasks containing 200mL soybean lecithin liquid medium, culture at 28°C and 150rpm constant temperature shaking, take samples at regular intervals, centrifuge at 8000rpm for 5min, and measure soluble phosphorus by molybdenum antimony anti-colorimetric method Each treatment was repeated three times, and the non-inoculated solution was used as the control.

[0061] Depend on Figure 4 A shows that there are obvious hydrolysis circles around MEM40 on the lecithin plate, indicating that it has the ability to degrade lecithin. Lecithin is hydrolyzed into glycerol, fatty acid, phosphoric acid and ...

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Abstract

The invention discloses a multifunctional rahnella aquatilis MEM40 strain and application thereof, and belongs to the technical field of microorganisms. A taxonomy name of the multifunctional rahnellaaquatilis MEM40 strain is Rahnella aquatilis MEM40, and a preservation number is CCTCC NO.M 2018463. The strain has functions of efficient phosphorus resolving and nitrogen fixation, the available phosphorus content and nitrogen content needed for growth of crops in soil can be improved, IAA secreted by the multifunctional rahnella aquatilis MEM40 strain can promote crop growth, moreover, the strain can generate multiple kinds of soil enzymes to accelerate substance and capability metabolism in the soil, then the soil fertility is improved, the soil state is improved, crop growth is promoted,use of fertilizer, especially phosphate fertilizer and nitrogen fertilizer, is reduced, and the planting cost is reduced.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a strain of multifunctional Lahnella water MEM40 and its application. Background technique [0002] Phosphorus is one of the nutrient elements necessary for crop growth. Most of the phosphorus in the soil of our country exists in the form of insoluble phosphate, which has stable chemical properties and is difficult to be absorbed and utilized by crops. Phosphorus fertilizer is used to increase the content of available phosphorus in the soil, but the inorganic phosphorus applied to the soil will combine with metal ions in the soil to form insoluble phosphate, resulting in low utilization rate of phosphorus fertilizer; and the abuse of phosphorus fertilizer not only causes waste of resources, but also It will bring about serious environmental problems, such as soil compaction, acidification, soil fertility decline, reduction in the quality of agricultural products, reduction in t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00C05G3/04C12R1/01C05G3/80
CPCA01N63/00C05F11/08C05G3/80C12N1/205C12R2001/01Y02P60/21
Inventor 倪红陈玲马卫孙孝文许银
Owner 湖北楚峰源种植有限公司
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