Preparation method of major yolk proteins of sea urchins

A technology of yolk protein and sea urchin eggs, which is applied in the field of preparation of main yolk protein of sea urchins, can solve the problems of single processed products and low high-value utilization, and achieve high productivity, improved processing utilization, and simple and effective preparation process

Inactive Publication Date: 2019-04-12
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] In the process of sea urchin processing, the processed sea urchin yolk products are single, and the yolk protein content of yellow sea urchin, Ezoma manure sea urchin and Dalian purple sea urchin is rich, up to about 68.55%, 60.21%, and 62.24% (by dry weight), but its high Value utilization is relatively low

Method used

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  • Preparation method of major yolk proteins of sea urchins
  • Preparation method of major yolk proteins of sea urchins
  • Preparation method of major yolk proteins of sea urchins

Examples

Experimental program
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Effect test

Embodiment 1

[0035] S1. Sea urchin egg yolk degreasing treatment: under the condition of 4°C, crack the shell of Dalian purple sea urchin, take the egg yolk, and homogenize the egg yolk homogenate to a yolk homogenate without particles. The ratio of n-hexane / absolute ethanol mixture (V 正己烷 :V 乙醇 =3:1) mixed, magnetically stirred for 6h, removed the organic reagent by suction filtration, and obtained a filter cake; the gained filter cake was mixed with n-hexane / dehydrated ethanol mixed solution (V 正己烷 :V 乙醇 = 3:1) mixed, magnetically stirred for 6 hours, and suction filtered to remove the organic reagent to obtain a filter cake; the resulting filter cake was washed with n-hexane / absolute ethanol mixture, dispersed, placed in normal temperature and naturally air-dried to obtain Dalian Purple Sea Urchin Egg yolk defatted powder; the content of protein in the obtained Dalian purple sea urchin egg yolk defatted powder measured by Kjeldahl method is 62.24% (by dry weight);

[0036] S2. Alkali...

Embodiment 2

[0042] S1. Sea urchin egg yolk degreasing treatment: under the condition of 4°C, the yellow sea urchin was broken, the egg yolk was taken, and the egg yolk homogenate was homogenized to a yolk homogenate without particles, and the egg yolk homogenate was mixed at a ratio of 1:10 (m / v). Ratio and n-hexane / absolute ethanol mixture (V 正己烷 :V 乙醇 =3:1) mixed, magnetically stirred for 6h, removed the organic reagent by suction filtration, and obtained a filter cake; the gained filter cake was mixed with n-hexane / dehydrated ethanol mixed solution (V 正己烷 :V 乙醇 = 3:1) mixed, magnetically stirred for 6 hours, and the organic reagent was removed by suction filtration to obtain a filter cake; the resulting filter cake was washed with n-hexane / absolute ethanol mixture, dispersed, and naturally air-dried at room temperature to obtain yellow sea urchin yolk Defatting powder; adopting the Kjeldahl method to measure the protein content in the obtained yellow sea urchin egg yolk defatting pow...

Embodiment 3

[0049] S1. Sea urchin egg yolk degreasing treatment: under the condition of 4°C, break the shell of Ezoma manure sea urchin, take the egg yolk, and homogenize the egg yolk homogenate to a yolk homogenate without particles, and prepare the egg yolk homogenate at a ratio of 1:10 (m / The ratio of v) and n-hexane / absolute ethanol mixed solution (V 正己烷 :V 乙醇 =3:1) mixed, magnetically stirred for 6h, removed the organic reagent by suction filtration, and obtained a filter cake; the gained filter cake was mixed with n-hexane / dehydrated ethanol mixed solution (V 正己烷 :V 乙醇 = 3:1) mixed, magnetically stirred for 6h, and the organic reagent was removed by suction filtration to obtain a filter cake; the obtained filter cake was washed with n-hexane / absolute ethanol mixture and dispersed, and then naturally air-dried at room temperature to obtain Ezoma Dung sea urchin egg yolk defatted powder; the content of protein in the obtained Ezoma dung sea urchin egg yolk defatted powder measured ...

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Abstract

The invention discloses a preparation method of major yolk proteins of sea urchins. The preparation method of the major yolk proteins of sea urchins takes yolk of sea urchins as a raw material, and comprises the following steps: preparing skimmed sea urchin yolk powder; carrying out alkali dissolving, acid precipitating and low-temperature desalinating by performing dialysis; and carrying out freeze-drying so as to obtain the main yolk proteins. By utilizing the yolk of sea urchins as the raw material, the preparation method of the major yolk proteins of sea urchins improves processing and utilization rates of the sea urchin yolk; and thus, protein resources of the sea urchin yolk are fully developed and utilized. The skimmed sea urchin yolk powder is prepared by adopting food-grade organic reagents n-hexane and anhydrous alcohol, so that, the operation is convenient and safe; moreover, the preparation steps are simple and effective. The main yolk proteins in the skimmed sea urchin yolk powder are dissolved and precipitated by using a sodium hydroxide solution and a hydrochloric acid solution; and then, neutralization reaction and dialysis desalination are performed, so that, application of the obtained major yolk proteins of sea urchins as a functional base material in foods can be guaranteed. Thus, the preparation method is of great significance to development and utilizationof sea urchin yolk resources.

Description

technical field [0001] The invention relates to the technical field of food processing, in particular to a method for preparing sea urchin main vitellin. Background technique [0002] In the process of sea urchin processing, the processed sea urchin yolk products are single, and the yolk protein content of yellow sea urchin, Ezoma manure sea urchin and Dalian purple sea urchin is rich, up to about 68.55%, 60.21%, and 62.24% (by dry weight), but its high Value utilization is relatively low. [0003] The preparation of the main yolk protein in the yolk of yellow sea urchin, Ezoma dung sea urchin and Dalian purple sea urchin can improve the utilization rate of yellow sea urchin yolk, has high social and economic value, and will have a good application prospect in the field of food. Contents of the invention [0004] The purpose of the present invention is to disclose a preparation method of the main yolk protein of sea urchin, improve the utilization rate of sea urchin yolk ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435C07K1/14C07K1/30
CPCC07K14/43504
Inventor 吴海涛商文慧韩佳润杜椅楠阎佳楠崔小凡薛聿函
Owner DALIAN POLYTECHNIC UNIVERSITY
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