Device for biological ex-situ degradation of organic matter
An organic matter and heterotopic technology, which is applied in the field of organic pollutant degradation, can solve the problems of cumbersome steps, large quantities, and unsafety, and achieve the effects of simple and convenient operation, low energy consumption, and low cost
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Embodiment 1
[0033] In-situ biodegradation of formaldehyde is carried out in two ways, one is the microbial suspension growth system, and the other is the biofilm system. The steps are as follows:
[0034] 1) Suspension growth system is to take the sewage from the sewage treatment plant as the source of microorganisms, and add the nutrients required by the microorganisms [sodium acetate 1.0g L -1 , phosphate buffer (50mM PBS, NH 4 Cl 0.31g L -1 ,KCl 0.13g·L -1 ,NaH 2 PO 4 2H 2 O2.772g·L -1 , Na 2 HPO 4 4.576g·L -1 ), metal solution 12.5mL·L -1 , and vitamin solution 5mL·L -1 ], expose oxygen to the solution for 10min, add high-concentration formaldehyde solution to make the concentration respectively 6, 8, 10 and 20mg / L, seal with tinfoil paper and plastic wrap, and treat for 24h.
[0035] 2) The biofilm system is also the sewage from the sewage treatment plant as the source of the carbon cloth to enrich the microorganisms. Add the above-mentioned nutrients required by the micr...
Embodiment 2
[0038] Adopt the present invention, synthesize H based on microbial electrochemical system 2 o 2 And realize the steps of the method for bioheterogeneous degradation of formaldehyde, specifically as follows:
[0039] 1) Install the graphite-carbon black mixed roll-pressed air cathode into the cathode chamber of an anode-acclimated microbial electrochemical system. The two chambers are separated by a cation exchange membrane to form a double-chamber BES. The cathode chamber is 50mM Na 2 SO 4 Solution, cultivated for two cycles to adapt the anode organisms to the environment.
[0040] 2) Apply a bias voltage of 0.6V between the cathode and anode for 1h, and pump the accumulated H in the cathode chamber 2 o 2 Solution transfer to UV / H 2 o 2 In the reaction vessel, add high-concentration formaldehyde solution dropwise to make the concentrations 6mg / L, 8mg / L, 10mg / L and 20mg / L respectively, adjust the pH of the solution to 3, set the optical path to 2cm and 5cm, turn on the s...
Embodiment 3
[0045] Adopt the present invention, synthesize H based on microbial electrochemical system 2 o 2 And realize the steps of the method for bioheterogeneous degradation of formaldehyde, specifically as follows:
[0046] 1) Install the graphite-carbon black mixed roll-pressed air cathode into the cathode chamber of an anode-acclimated microbial electrochemical system. The two chambers are separated by a cation exchange membrane to form a double-chamber BES. The cathode chamber is 50mM Na 2 SO 4 Solution, cultivated for two cycles to adapt the anode organisms to the environment.
[0047] 2) Apply 0.6V bias voltage 0h, 0.5h, 1.0h and 1.5h between the cathode and anode, and use the pump to transfer the hydrogen peroxide solution accumulated in the cathode chamber for 0h, 0.5h, 1.0h and 1.5h to the UV / h 2 o 2 In the reaction vessel, add high-concentration formaldehyde solution dropwise to make the concentration 6mg / L, adjust the pH of the solution to 3, set the optical path to 2...
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