SNP marker developing and application of rice blast resistance gene Pigm of rice

Abstract

The invention provides an SNP molecular marker K_060502 which is tightly linked to a broad-spectrum rice blast resistance gene Pi2. It is detected by the SNP marker that the basic group at the 10432621 site of the 6 chromosome of rice is G or T, and the primer combination of the SNP marker developed based on a KASP technology is Primer X, Primer Y and Primer C. According to the SNP marker,the KASP technology is utilized to perform rapid genotyping on the SNP marker which is tightly linked to the broad-spectrum rice blast resistance gene Pi2, the SNP marker can be applied to commercialmolecular seedling breeding with high, medium or low through-put, the selectivity efficiency of a developed SNP marker phenotype reaches up to 100%, and therefore the broad-spectrum rice blast resistance gene Pi2 can be rapidly and accurately detected in different germ plasm resources of indica rice, japonica rice and the like; the complicated procedures of enzyme digestion, electrophoresis, sequencing and the like are not needed in the detecting process, aerosol contamination is reduced, the use of toxic substances of ethidium bromide EB (ethidium bromide) and the like is reduced, outlook molecular marker auxiliary selection at the early stage of seedling breeding can be conducted to reduce the field planting scale of seedling breeding groups and the seedling breeding cost, and the seedling breeding process is accelerated.

Description

technical field [0001] The invention relates to the fields of molecular markers and crop breeding, in particular to the development and application of a SNP marker of rice blast resistance gene Pi2. Background technique [0002] Rice is one of the most important food crops in China and even in the world. More than half of the world's population uses rice as a staple food. Rice blast, known as the cancer of rice, is one of the most serious diseases to rice production. The hybrid rice widely promoted in China lacks the introduction of high-quality disease-resistant resources from distant sources, and the genetic diversity of the parents is not high. The improvement of disease resistance is a major bottleneck; in major rice-growing countries, there are often reports of rice blast epidemics and outbreaks. Yield losses of 20-100% can be caused annually. [0003] Practice has proved that breeding disease-resistant varieties is the most economical, safe, environmentally friendly ...

AI Technical Summary

Problems solved by technology

[0006] Currently developed molecular markers generally require agarose gel electrophoresis (Deng Guofu et al., CN105462971 B; Zhang Mingyong et al., CN106636358 A), and even more complex polyacrylamide gel electrophoresis (Chen Qingquan et al., CN 102312000 A Chen Songbiao, etc., CN 104073487 B; Ji Jianhui, etc., CN 104450932 B; Sheng Zhonghua, etc., CN106636395 A), some also need to carry out polyacrylamide gel electrophoresis after enzymatic digestion (Hua Xia, etc., CN 103320437 B), the operation is loaded down with trivial details, Time-consuming and material-consuming, not convenient for large-scale identification; Jin Mingzhen et al. (CN 107435068A) detect SNP (Single Nucleotide Polymorphism) sites by the method of high resolution melting curve analysis (High Resolution Melting Curve Analysis, HRM), although avoiding electrophoresis or Enzyme digestion process, but as the amplification product fragments increase, the accuracy and stability of the detection will gradually decrease

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