A method for removing bacterial cells in thermogel fermented liquid
A technology of bacterial cells and thermal gel, which is applied in the field of bioengineering, can solve the problems of reduced filtration speed, poor fluidity, and difficulty in separating and removing microbial cells, and achieve the effect of improving processing efficiency
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Embodiment 1
[0040] according to figure 2 The process shown, the method of pressure filtration to remove bacterial cells: collect 7.5L fermented thermal gel fermentation liquid (the thermal gel content is 40g L -1 ) to perform plate and frame press filtration, remove the filtrate, and obtain a wet mixture of thermogel and thalline; dissolve the resulting wet mixture in 30L 0.5mol L -1 In NaOH solution, the final concentration of thermogel is 10g L -1 , using a mechanical stirrer at 600r·min -1 Stir at a stirring speed of 1 h, and then use GEANiro high-pressure homogenizer to homogenize the mixed alkali sol, the homogenization pressure is 50MPa, and the flow rate is 3L min -1 , using the method of microscopic examination to detect that there is no insoluble colloid in the alkali sol, the alkali sol obtained after complete dissolution is used for the filter press experiment, and 400g of fast perlite is added to the 30L alkali sol obtained after homogenization, and fully mixed Stir to sus...
Embodiment 2
[0044] The method for removing thalline cells by pressure filtration: collect 1000L of fermented thermal gel fermented liquid (the thermal gel content is 60g L -1 ) to carry out diaphragm plate and frame press filtration, remove the filtrate, obtain the wet mixture of thermal gel and thalline, dissolve in 3000L0.7mol L -1 In the KOH solution, the final concentration of thermogel is 20g L -1 , using a mechanical stirrer at 100r·min -1 Stir at a stirring speed of 1h, then use a high-pressure homogenizer for homogenization, the homogenization pressure is 40MP, and the flow rate is 10m 3 h -1 , using a microscope to detect that there is no insoluble colloid in the alkali sol, the alkali sol obtained after dissolution is used for press filtration, and 30kg of diatomaceous earth is added to the 3000L hot gel alkali sol obtained after homogenization, and fully mixed Stir and suspend; take another 10kg diatomaceous earth and 1000L 0.7mol L -1 The KOH solution is mixed as the pre-c...
Embodiment 3
[0047] Collect 10m 3 Fermentation gained thermogel fermented liquid (the thermogel content is 55g L -1 ) to carry out diaphragm plate and frame press filtration, remove the filtrate, and obtain a wet mixture of thermal gel and bacteria, which is dissolved in 30m 3 0.5mol·L -1 In NaOH solution, the final concentration of thermogel is 18.3g L -1 , using a mechanical stirrer at 100r·min -1 Stir at a stirring speed of 1h, then use a high-pressure homogenizer for homogenization, the homogenization pressure is 40MP, and the flow rate is 10m 3 h -1 , using the method of microscopic examination, it was found that there was no insoluble colloid in the alkali sol, and the alkali sol obtained after dissolution was used for press filtration, and the 30m 3 Add 300kg of diatomite to the alkali sol, mix well and stir to suspend; take another 20kg of diatomite and 2000L 0.5mol·L -1 The NaOH solution is mixed as the pre-coated diatomite. After the pretreatment of the filter press, the p...
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