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Screening method for lactobacillus acidophilus amino acid requirements

A Lactobacillus acidophilus, amino acid technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as being unsuitable for large-scale screening of enterprises, high cost, and complicated preparation.

Pending Publication Date: 2019-04-19
BIOGROWING CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the growth factors of Lactobacillus acidophilus include more than ten kinds of nutrients such as purine, pyrimidine and vitamins, the preparation is complicated and the cost is high. Large-Scale Screening of Nutritional Requirements of Amino Acids of Lactobacillus acidum

Method used

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  • Screening method for lactobacillus acidophilus amino acid requirements
  • Screening method for lactobacillus acidophilus amino acid requirements
  • Screening method for lactobacillus acidophilus amino acid requirements

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Inoculate the Lactobacillus acidophilus strain 007 frozen at -80°C in the basal medium (basic medium: glucose: 20g / L, peptone: 10g / L, beef extract powder: 10g / L, yeast extract: 5g / L, Dipotassium hydrogen phosphate: 2g / L, diamine hydrogen citrate: 2g / L, sodium acetate: 5g / L, magnesium sulfate heptahydrate: 0.25g / L, manganese sulfate monohydrate: 0.05g / L, Tween-80 : 1g / L) at 37°C for 12h. The activated seed culture medium was taken with 1% inoculum volume, centrifuged to remove the medium, and then resuspended in an equal amount of sterile water for inoculation.

[0057] Prepare control medium: glucose: 30g / L, yeast extract: 0.1g / L, tryptophan: 0.1g / L, asparagine: 0.1g / L, isoleucine: 0.1g / L, lysine : 0.1g / L, glycine: 0.1g / L, leucine: 0.1g / L, serine: 0.1g / L, histidine: 0.1g / L, tyrosine: 0.1g / L, cysteine : 0.1g / L, Valine: 0.1g / L, Phenylalanine: 0.1g / L, Aspartic Acid: 0.1g / L, Glutamic Acid: 0.1g / L, Threonine: 0.1g / L, alanine: 0.1g / L, methionine: 0.1g / L, proline: 0.1g / L, ...

Embodiment 2

[0065] Inoculate the Lactobacillus acidophilus strain 008 frozen at -80°C in the basal medium (basic medium: glucose: 20g / L, peptone: 10g / L, beef extract powder: 10g / L, yeast extract: 5g / L, Dipotassium hydrogen phosphate: 2g / L, diamine hydrogen citrate: 2g / L, sodium acetate: 5g / L, magnesium sulfate heptahydrate: 0.25g / L, manganese sulfate monohydrate: 0.05g / L, Tween-80 : 1g / L) at 37°C for 12h. The activated seed culture medium was taken with 1% inoculum volume, centrifuged to remove the medium, and then resuspended in an equal amount of sterile water for inoculation.

[0066] Preparation of control medium: glucose: 20g / L, yeast extract: 0.25g / L, tryptophan: 0.1g / L, asparagine: 0.1g / L, isoleucine: 0.1g / L, lysine : 0.1g / L, Glycine: 0.1g / L, Leucine: 0.1g / L, Serine: 0.1g / L, Histidine: 0.1g / L, Tyrosine: 0.1g / L, Cysteine Acid: 0.1g / L, Valine: 0.1g / L, Phenylalanine: 0.1g / L, Aspartic Acid: 0.1g / L, Glutamic Acid: 0.1g / L, Threonine: 0.1 g / L, alanine: 0.1g / L, methionine: 0.1g / L, proli...

Embodiment 3

[0073] Inoculate the Lactobacillus acidophilus strain 009 frozen at -80°C into the basal medium (basic medium: glucose: 20g / L, peptone: 10g / L, beef extract powder: 10g / L, yeast extract: 5g / L, Dipotassium hydrogen phosphate: 2g / L, diamine hydrogen citrate: 2g / L, sodium acetate: 5g / L, magnesium sulfate heptahydrate: 0.25g / L, manganese sulfate monohydrate: 0.05g / L, Tween-80 : 1g / L) at 37°C for 12h. The activated seed culture medium was taken with 1% inoculum volume, centrifuged to remove the medium, and then resuspended in an equal amount of sterile water for inoculation.

[0074] Preparation of control medium: glucose: 20g / L, yeast extract: 0.1g / L, tryptophan: 0.1g / L, asparagine: 0.1g / L, isoleucine: 0.1g / L, lysine : 0.1g / L, Glycine: 0.1g / L, Leucine: 0.1g / L, Serine: 0.1g / L, Histidine: 0.1g / L, Tyrosine: 0.1g / L, Cysteine Acid: 0.1g / L, Valine: 0.1g / L, Phenylalanine: 0.1g / L, Aspartic Acid: 0.1g / L, Glutamic Acid: 0.1g / L, Threonine: 0.1 g / L, alanine: 0.1g / L, methionine: 0.1g / L, prol...

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Abstract

The invention relates to the field of biotechnology, in particular to a screening method for lactobacillus acidophilus amino acid requirements. The screening method for lactobacillus acidophilus aminoacid requirements comprises the following steps that a basic culture medium is prepared, wherein lactobacillus acidophilus strains freezing-preserved at -80 DEG C are inoculated into the basic culture medium; liquid is extracted from an activated seed culture medium with the inoculation amount being 1%; a control culture medium is prepared, the pH value of the culture medium is adjusted to be 6.5+ / -0.1, sterilizing is conducted for 20 min at 121 DEG C; while the control culture medium is prepared, 19 defect inspection media lacking one of 19 kinds of amino acid respectively are prepared separately; all data is observed and preserved. Compared with the prior art, the special amino acid requirements of different lactobacillus acidophilus are determined by comparing the final OD600 with thepH.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a screening method for amino acid requirements of Lactobacillus acidophilus. Background technique [0002] Lactobacillus acidophilus is a common probiotic and has many probiotic functions, such as regulating intestinal health, anti-oxidation, anti-fungal, etc., and has good acid and bile salt resistance, and can survive in the intestinal tract. The market demand for Lactobacillus acidophilus is huge, and the high-density fermentation of Lactobacillus acidophilus is an important issue in the production of Lactobacillus acidophilus products with high viable counts. The nutritional requirements of Lactobacillus acidophilus are complex, and the amino acid nutritional requirements of different Lactobacillus acidophilus strains vary greatly. At present, there is no report on the technology of screening the amino acid nutritional requirements of Lactobacillus acidophilus. Existing literatu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02
CPCC12Q1/02
Inventor 宋锦安徐煜
Owner BIOGROWING CO LTD
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