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Lactobacillus reuteri high-density fermentation medium and fermentation method and application

A Lactobacillus reuteri, high-density fermentation technology, applied in the field of microbial fermentation, can solve problems such as bad flavor, achieve the effects of improving the flavor of the culture medium, simplifying the process flow, and improving the flavor of the fermentation broth

Active Publication Date: 2019-04-23
HANGZHOU WAHAHA TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the typical bad flavor of the traditional Lactobacillus reuteri culture medium after fermentation, the present invention provides a high-density fermentation medium for Lactobacillus reuteri that can simultaneously improve the fermentation flavor and increase the number of viable fermentation bacteria

Method used

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  • Lactobacillus reuteri high-density fermentation medium and fermentation method and application

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Embodiment 1

[0031] (1) Inoculate the Lactobacillus reuteri strain in the seed medium, culture it anaerobically at 37° C. for 16 hours, and cultivate the seed solution; the seed medium is a traditional MRS medium, and each liter of the seed medium includes: glucose 20g, tryptone 5g, beef extract 10g, yeast powder 50g, soytone 5g, manganese sulfate 0.58g, magnesium sulfate 0.28g, Tween 80 0.1g, cysteine ​​hydrochloride 0.5g, dipotassium hydrogen phosphate 2g, Diamine hydrogen citrate 2g and sodium acetate 10g;

[0032] (2) Inoculate the seed solution in a 6L Lactobacillus reuteri high-density fermentation medium (a fermenter with a capacity of 10L) at an inoculum size of 5% (v / v), and feed CO at 37°C. 2, the stirring speed is controlled at 120rpm, and the fermentation culture is carried out under anaerobic stirring conditions for 20 hours; each liter of Lactobacillus reuteri high-density fermentation medium includes the following raw materials: 30g of maltose, 80g of soybean peptone, 65g of...

Embodiment 2

[0034] (1) Inoculate the Lactobacillus reuteri strain in the seed medium, culture it anaerobically at 37° C. for 15 hours, and cultivate the seed solution; the seed medium is a traditional MRS medium, and each liter of the seed medium includes: glucose 20g, tryptone 5g, beef extract 10g, yeast powder 50g, soytone 5g, manganese sulfate 0.58g, magnesium sulfate 0.28g, Tween 80 0.1g, cysteine ​​hydrochloride 0.5g, dipotassium hydrogen phosphate 2g, Diamine hydrogen citrate 2g and sodium acetate 10g;

[0035] (2) Inoculate the seed solution with an inoculum amount of 1% (v / v) in 80ml Lactobacillus reuteri high-density fermentation medium (blue cap bottle with a capacity of 100ml), and pass CO under the condition of 35°C. 2 , the stirring speed is controlled at 130rpm, and the fermentation culture is carried out under anaerobic stirring conditions for 24 hours; each liter of Lactobacillus reuteri high-density fermentation medium includes the following raw materials: maltose 10g, so...

Embodiment 3

[0037] (1) Inoculate the Lactobacillus reuteri strain in the seed medium, culture it anaerobically at 37° C. for 12 hours, and cultivate the seed solution; the seed medium is a traditional MRS medium, and each liter of the seed medium includes: glucose 20g, tryptone 5g, beef extract 10g, yeast powder 50g, soytone 5g, manganese sulfate 0.58g, magnesium sulfate 0.28g, Tween 80 0.1g, cysteine ​​hydrochloride 0.5g, dipotassium hydrogen phosphate 2g, Diamine hydrogen citrate 2g and sodium acetate 10g;

[0038] (2) Inoculate the seed liquid in the high-density fermentation medium of Lactobacillus reuteri with an inoculum size of 10% (v / v), and at 38°C, pass CO 2 , the stirring speed is controlled at 100rpm, and the fermentation culture is carried out under anaerobic stirring conditions for 16 hours; each liter of Lactobacillus reuteri high-density fermentation medium includes the following raw materials: 50g of maltose, 60g of soybean peptone, 30g of malt extract powder, 15g of yeas...

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Abstract

The invention relates to the technical field of microbial fermentation, and provides a lactobacillus reuteri high-density fermentation medium and fermentation method and application in order to solvethe problem that typical bad flavor appears after a traditional lactobacillus reuteri medium is fermented. Each liter of lactobacillus reuteri high-density fermentation medium is prepared from 10-50 gof maltose, 20-80 g of soybean peptone and 30-100 g of malt extract powder. Accordingly, after the medium is fermented, the content of ketones, lipids and other aromatic substances in the flavor is obviously increased, the number of viable bacteria is increased by 43 times, the pH does not need to be adjusted in the fermentation process, and the medium is beneficial to large-scale industrial production; the obtained viable bacterial fermentation broth can be directly added in fermented yoghurt without the high energy consumption freeze-drying process, and the bad flavor of the product is notcaused.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a high-density fermentation medium and fermentation method of Lactobacillus reuteri. Background technique [0002] Lactobacillus reuteri is a kind of probiotics that are commonly colonized in the intestinal tract of humans and animals. It has the beneficial effects of improving immunity, regulating intestinal flora, and improving colic in infants. Lactobacillus reuteri WHH1689 has the characteristics of long shelf life at room temperature when added to dairy products, and has a significant improvement effect on irritable bowel syndrome. [0003] At present, the medium for cultivating Lactobacillus reuteri at home and abroad basically adopts the traditional MRS medium, and the number of viable bacteria is generally 1 to 5×10 9 cfu / ml, and has a typical bad flavor after fermentation. The existing lactic acid bacteria biological products are finally presented in the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/225
CPCC12N1/20
Inventor 陈苏陈丽娥陈琳李言郡李宝磊康国凯陈作国任学良周晴晴朱珺
Owner HANGZHOU WAHAHA TECH
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