Biological organic fertilizer with water-retention and drought-resisting functions and preparation method thereof

A bio-organic fertilizer and microbial strain technology, applied in the field of agricultural biology, can solve the problems of affecting plant water and nutrient absorption, reducing the diversity of community structure, reducing the number of soil bacteria, etc., to promote colonization and reproduction, and improve survival rate , Improve the effect of drought stress resistance

Active Publication Date: 2019-04-26
SHANDONG FOREST SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In arid habitats, a large number of microorganisms cannot successfully colonize and survive, resulting in a significant reduction in the number of soil bacteria and a significant decrease in the diversity of

Method used

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  • Biological organic fertilizer with water-retention and drought-resisting functions and preparation method thereof
  • Biological organic fertilizer with water-retention and drought-resisting functions and preparation method thereof
  • Biological organic fertilizer with water-retention and drought-resisting functions and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0033] Embodiment 1 Bacillus subtilis (Bacillus subtilis) GE3 identification

[0034] The Bacillus subtilis disclosed in this disclosure is obtained through screening by the applicant.

[0035] The colony characteristics of the plant rhizosphere growth-promoting bacteria are: the GE3 bacterial colony on the LB medium is irregular in shape, the colony size is about 5mm after 24 hours of cultivation, the surface is dry and dull, and the edges are irregular, and the colony is brownish-yellow and intermediate in color. Darker, opaque, with spores.

[0036] The 16S rRNA gene sequence of GE3 was compared with the sequence in several Genbank databases by BLAST analysis, and the results showed that: GE3 and Bacillus subtilis belonged to the same smallest branch, and the 16S rRNA gene sequence of DZ1 and Bacillus subtilis (AJ276351) had the closest evolutionary distance. The similarity reaches 98.92%. Based on the physiological and biochemical characteristics and colony morphological c...

Embodiment 2

[0046] Embodiment 2 A kind of drought-resistant solid bacterial agent

[0047] The preparation method of the drought-resistant solid bacterial agent is:

[0048] (1) The preparation method of activated humic acid is to mix and react lignite humic acid raw material and 20-25% nitric acid according to the ratio of 2:1 for 40 minutes, centrifuge off the nitric acid solution, then add tap water, centrifuge, and repeat the operation until centrifugation Liquid pH>5.5, dry.

[0049] (2) Preparation of Bacillus subtilis GE3 fermentation broth:

[0050] ① Seed culture in test tube

[0051] Seed medium: 0.3% beef extract, 1% peptone, 0.5% sodium chloride, pH7.0-7.2.

[0052] Bacillus subtilis G3 is inoculated on the culture medium and cultured at a constant temperature of 30-35° C. to obtain a test tube species.

[0053] ② Liquid fermentation culture

[0054] The formula of the liquid fermentation medium is: 3% sucrose, 2% yeast extract, K 2 HPO 4 0.10%, MgSO 4 ·7H 2 O0.01%, ...

Embodiment 3

[0062] Embodiment 3 A kind of water-retaining and drought-resistant biological organic fertilizer

[0063] The water-retaining and drought-resistant bio-organic fertilizer is composed of solid drought-resistant bacteria agent, water-retaining agent and fermented water-washed cow dung in a mass ratio of 1:0.1:20; the solid drought-resistant bacteria agent is composed of activated humic acid and Bacillus subtilis The microbial fermentation broth of GE3 is mixed according to the material-to-liquid ratio of 5:1kg / L.

[0064] The drought-resistant bio-organic fertilizer contains a total effective number of viable bacteria ≥ 0.3×10 8 cfu / g.

[0065] The preparation method of the water-retaining and drought-resistant bio-organic fertilizer:

[0066] (1) The method for making the fermented water-washed cow dung is as follows: the raw material of the washed cow dung is decomposed by strip-stack fermentation, the stack height is > 1m, the width is greater than 3m, and the length is > ...

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Abstract

The invention relates to the technical field of agricultural biology and in particular relates to a biological organic fertilizer with water-retention and drought-resisting functions and a preparationmethod thereof. The biological organic fertilizer contains bacillus subtilis GE3; the preservation number of the bacillus subtilis GE3 is CGMCC (China General Microbiological Culture Collection Center) No. 11966. The strain not only has a relatively strong colonization capability in a drought environment, but also can be used for remarkably improving the survival rate of forest trees in the drought environment and promoting the growth of the forest trees. The water-retention and drought-resisting biological organic fertilizer provided by the invention takes a solid drought-resisting bacteriumagent containing the bacillus subtilis GE3 and fermented water-washed cow dung as main components and a small amount of a water-retention agent is added; all components have a mutual effect to extremely promote the growth of the forest trees in the drought environment, so that the drought stress resisting capability of the forest trees is improved and the survival rate of the forest trees in thedrought environment is improved.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a bio-organic fertilizer with water retention and drought resistance functions and a preparation method thereof. Background technique [0002] Bio-organic fertilizer refers to a type of microbial fertilizer and organic fertilizer that is composed of specific functional microorganisms and organic materials that are mainly sourced from animal and plant residues (such as livestock and poultry manure, crop straw, etc.) and have been harmlessly treated and decomposed. Fertilizer with fertilizer effect. However, a large number of studies have shown that the interaction between functional microorganisms and crops is often unstable, and this instability stems from the impact of different environmental conditions on the growth of microorganisms, thereby restricting their effects. This requires the functional microorganisms in microbial fertilizers to adapt to the external enviro...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/04C05F11/08C12R1/125C05G3/80
CPCC05F3/00C05F11/08C12N1/20C05G3/80C12N1/205C12R2001/125C05F11/02
Inventor 刘幸红马海林刘方春丁延芹杜秉海刘丙花马丙尧杜振宇彭琳李丽井大炜吕雷昌张文馨
Owner SHANDONG FOREST SCI RES INST
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