Use of a compound dehydrogenase
A technology for rooting hormones, compounds, applied in the direction of plant growth regulators, microorganism-based methods, applications, etc.
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Embodiment 1
[0030] The compound dehydroradicinin (Dehydroradicinin) isolated and extracted from Curvularia secondary metabolites, including the fermentation of strains, the extraction and separation and purification of secondary metabolites, the specific operations are carried out according to the following steps:
[0031] Fermentation of strains:
[0032] a. Transplant the Curvularia species into the test tube PDA slant medium for activation, move the activated Curvularia into the PDA plate medium for cultivation, and then use a 5mm diameter punch to take the Curvularia cake and insert it into potatoes In the glucose culture solution, the insertion amount is 2 pieces / 100mL, put it into the shaking table and shake it to obtain the fermentation solution;
[0033] b. Separate the mycelium and the fermentation broth with four layers of gauze and filter paper for the fermentation broth obtained in step a, then add the same volume of ethyl acetate to the fermentation broth and stir and extract...
Embodiment 2
[0036] The compound dehydroradicinin (Dehydroradicinin) extracted from Curvularia secondary metabolites is used as a herbicide:
[0037] The compound dehydroradicinin with a purity of 98% after purification was prepared into a 500 μg / mL solution with acetone, and then diluted to 1 μg / mL, 5 μg / mL, 20 μg / mL, and 100 μg / mL solutions in turn. Place filter paper in a 6 cm petri dish, add 3 mL of dehydroradicinin (Dehydroradicinin) solution with a concentration of 1 μg / mL, 5 μg / mL, 20 μg / mL, 100 μg / mL and 500 μg / mL respectively, and wait until the acetone is completely volatilized. Add 3 mL of distilled water to each petri dish, and add 10 grains of retroflexus retroflexus, alfalfa, bluegrass, barnyardgrass and Italian cocklebur to each petri dish, each treatment is repeated three times, and the petri dish is sealed with parafilm. After they are ready, they are placed in a 25°C incubator and cultivated in the dark for 5 days, then measure the root length and seedling height of the s...
Embodiment 3
[0042] The compound dehydroradicinin extracted from the secondary metabolites of Curvularia sp. as a herbicide compared with the commercial herbicide glyphosate:
[0043] The compound dehydroradicinin with a purity of 98% after purification was prepared into a 500 μg / mL solution with acetone, and then diluted to 1 μg / mL, 5 μg / mL, 20 μg / mL, and 100 μg / mL solutions in turn. Place filter paper in a 6 cm petri dish, add 3 mL of dehydroradicinin (Dehydroradicinin) solution with a concentration of 1 μg / mL, 5 μg / mL, 20 μg / mL, 100 μg / mL and 500 μg / mL respectively, and wait until the acetone is completely volatilized. Add 3 mL of distilled water to each petri dish, and add 10 grains of retroflexus retroflexus, alfalfa, bluegrass, barnyardgrass and Italian cocklebur to each petri dish, each treatment is repeated three times, and the petri dish is sealed with parafilm. After they are ready, they are placed in a 25°C incubator and cultivated in the dark for 5 days, then measure the root l...
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