Use of a compound dehydrogenase

A technology for rooting hormones, compounds, applied in the direction of plant growth regulators, microorganism-based methods, applications, etc.

Active Publication Date: 2022-01-18
XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

By the purposes of the compound dehydrorhizotoxin of the present invention, as herbicide, the growth of common weeds is inhibited (phytotoxicity), and there is no report in domestic and foreign documents

Method used

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  • Use of a compound dehydrogenase
  • Use of a compound dehydrogenase
  • Use of a compound dehydrogenase

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0030] The compound dehydroradicinin (Dehydroradicinin) isolated and extracted from Curvularia secondary metabolites, including the fermentation of strains, the extraction and separation and purification of secondary metabolites, the specific operations are carried out according to the following steps:

[0031] Fermentation of strains:

[0032] a. Transplant the Curvularia species into the test tube PDA slant medium for activation, move the activated Curvularia into the PDA plate medium for cultivation, and then use a 5mm diameter punch to take the Curvularia cake and insert it into potatoes In the glucose culture solution, the insertion amount is 2 pieces / 100mL, put it into the shaking table and shake it to obtain the fermentation solution;

[0033] b. Separate the mycelium and the fermentation broth with four layers of gauze and filter paper for the fermentation broth obtained in step a, then add the same volume of ethyl acetate to the fermentation broth and stir and extract...

Embodiment 2

[0036] The compound dehydroradicinin (Dehydroradicinin) extracted from Curvularia secondary metabolites is used as a herbicide:

[0037] The compound dehydroradicinin with a purity of 98% after purification was prepared into a 500 μg / mL solution with acetone, and then diluted to 1 μg / mL, 5 μg / mL, 20 μg / mL, and 100 μg / mL solutions in turn. Place filter paper in a 6 cm petri dish, add 3 mL of dehydroradicinin (Dehydroradicinin) solution with a concentration of 1 μg / mL, 5 μg / mL, 20 μg / mL, 100 μg / mL and 500 μg / mL respectively, and wait until the acetone is completely volatilized. Add 3 mL of distilled water to each petri dish, and add 10 grains of retroflexus retroflexus, alfalfa, bluegrass, barnyardgrass and Italian cocklebur to each petri dish, each treatment is repeated three times, and the petri dish is sealed with parafilm. After they are ready, they are placed in a 25°C incubator and cultivated in the dark for 5 days, then measure the root length and seedling height of the s...

Embodiment 3

[0042] The compound dehydroradicinin extracted from the secondary metabolites of Curvularia sp. as a herbicide compared with the commercial herbicide glyphosate:

[0043] The compound dehydroradicinin with a purity of 98% after purification was prepared into a 500 μg / mL solution with acetone, and then diluted to 1 μg / mL, 5 μg / mL, 20 μg / mL, and 100 μg / mL solutions in turn. Place filter paper in a 6 cm petri dish, add 3 mL of dehydroradicinin (Dehydroradicinin) solution with a concentration of 1 μg / mL, 5 μg / mL, 20 μg / mL, 100 μg / mL and 500 μg / mL respectively, and wait until the acetone is completely volatilized. Add 3 mL of distilled water to each petri dish, and add 10 grains of retroflexus retroflexus, alfalfa, bluegrass, barnyardgrass and Italian cocklebur to each petri dish, each treatment is repeated three times, and the petri dish is sealed with parafilm. After they are ready, they are placed in a 25°C incubator and cultivated in the dark for 5 days, then measure the root l...

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Abstract

The invention discloses the use of a compound dehydrorhizotoxin, which is isolated and extracted from secondary metabolites of Curvularia sp., including the steps of fermentation of strains, extraction of secondary metabolites and separation and purification Complete, the purposes of the compound dehydroradicin in the preparation of herbicides, the compound dehydroradicin (Dehydroradicin) obtained by the present invention as a herbicide, in use, that is, when the concentration is 100 μg / mL, to Five common wild weeds that are difficult to eradicate with general herbicides, Amaranthus retroflexus, alfalfa, bluegrass, barnyardgrass, and the invasive plant Cocklebur sativa significantly inhibit the growth of seedlings, and they are prepared at different concentrations at the same time. Mixed with other pesticides, it is widely used in the development of various herbicides, and has great potential commercial application value.

Description

technical field [0001] The invention relates to extracting compounds from microorganisms as herbicides, in particular to the use of a compound dehydroradicinin extracted from Curvularia inaequalis secondary metabolites as herbicides. Background technique [0002] Chemical herbicides are widely used in agriculture, forestry and animal husbandry, but they often cause pollution to the natural environment. A variety of synthetic chemical herbicides tend to persist in nature for a long time due to the lack of corresponding microorganisms to decompose them, thus threatening the safety of soil and groundwater resources that human beings rely on for survival. However, natural ingredients extracted from nature are used as herbicides because they are easy to decompose and have no residue in nature, and they are increasingly valued. In addition, although some chemical herbicides can be decomposed in the soil, due to long-term and large-scale application, weeds gradually evolve resista...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/90A01P13/00C12P17/18C12R1/645
Inventor 邵华魏彩霞周世兴张弛韩彩霞梅宇刘铁
Owner XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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