Liquid fertilizer preventing fruit cracking of winter jujubes and preparation method of liquid fertilizer
A technology of liquid fertilizer and fruit cracking, applied in the field of liquid fertilizer, can solve the problems of stunted growth of various plant organs, restricting the planting and cultivation of winter jujube, and affecting fruit yield, etc., and achieves the effects of being beneficial to growth and development, preventing fruit cracking, and having a remarkable effect
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Embodiment 1
[0049] The isolation and identification of embodiment 1 Streptomyces bacterial strain (Streptomyces)
[0050] 1. Experimental materials and conditions
[0051] (1) Soil samples collected in a certain place in Chenzhou City, Hunan Province.
[0052] (2) The components per liter of the liquid medium are as follows: 3.0 g of beef extract, 10.0 g of peptone, 5.0 g of sodium chloride, dilute to 1 liter of water, and the pH is 7.5.
[0053] The components per liter of solid medium are as follows: 3.0g of beef extract, 10.0g of peptone, 5.0g of sodium chloride, 15-25g of agar, dilute to 1 liter of water, and the pH is 7.5.
[0054] The culture medium was divided into aliquots and sterilized in an autoclave (121°C, 30min).
[0055] (3) Sterile operating conditions: All utensils and utensils must be sterilized in an autoclave (121°C, 30min), and operations such as inoculation are carried out in an ultra-clean workbench.
[0056] (4) Conditions for culturing strains: place them in a ...
Embodiment 2
[0071] Embodiment 2 Preparation and Application of a Liquid Fertilizer for Preventing Fruit Cracking of Winter Jujube
[0072] A kind of preparation method of the liquid fertilizer that prevents winter jujube fruit cracking comprises the following steps:
[0073] S1. Preparation of culture medium: soak 1000g of soybeans for 10 hours, put them into a refiner and grind them into soybean milk, filter out the bean dregs, put the filtrate in a clean fermenter, add white sugar to the filtrate, add water to The concentration of sugar in the medium is 100g / L, and the pH is 7.5;
[0074] S2. inoculation: get the liquid spawn of Streptomyces bacterial strain " TY-001# ", inoculate in the culture medium, stir evenly; There are 200,000,000 viable bacteria per milliliter in the liquid spawn; The ratio of liquid spawn and culture medium 165g:250L;
[0075] S3. Fermentation: Turn on the oxygen pump, continuously feed oxygen into the fermenter during the fermentation process, and add an app...
Embodiment 3
[0082] Embodiment 3 A kind of preparation and application of the liquid fertilizer that prevents winter jujube from cracking fruit
[0083] A kind of preparation method of the liquid fertilizer that prevents winter jujube fruit cracking comprises the following steps:
[0084] S1. Preparation of culture medium: soak 1000g of soybeans for 10 hours, put them into a refiner and grind them into soybean milk, filter out the bean dregs, put the filtrate in a clean fermenter, add white sugar to the filtrate, add water to The concentration of sugar in the medium is 100g / L, and the pH is 7.5;
[0085] S2. inoculation: get the liquid spawn of Streptomyces bacterial strain " TY-001# ", inoculate in the culture medium, stir evenly; There are 200,000,000 viable bacteria per milliliter in the liquid spawn; The ratio of liquid spawn and culture medium 165g:250L;
[0086] S3. Fermentation: Turn on the oxygen pump, continuously feed oxygen into the fermenter during the fermentation process, a...
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