Carbon-drop guaiane sesquiterpene lactone compound and preparation method thereof
A technology of ester compound and guaiacol, applied in the field of medicine, can solve the problems of increasing the cytotoxicity of macrophages and the like
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Embodiment 1
[0038] a. Take 5 kg of the whole Achillea sylvestris, crush it and extract it with 50L of 50% ethanol-water solution at room temperature, then evaporate the solvent under reduced pressure to obtain a crude extract of the Achills sylvestris;
[0039] b. Disperse the crude extract obtained in step a with water, add petroleum ether, ethyl acetate, and n-butanol to extract 3 times in sequence, combine the ethyl acetate layers and evaporate to dryness under reduced pressure to obtain the ethyl acetate layer extract extract;
[0040] c. Separate the extract from the ethyl acetate layer obtained in step b with a normal phase silica gel column, and perform gradient elution with petroleum ether-ethyl acetate in a volume ratio of 50:1-0:1, and the fractions are passed through a thin silica gel layer Chromatographic analysis, the same fractions were combined to obtain 9 components F1-F9; the component F4 was separated by Sephadex LH-20 gel column and eluted with chloroform-methanol with a volu...
Embodiment 2
[0042] a. Take 5 kg of Achillea sylvestris whole plant, crush it and extract with 40L of 95% ethanol-water solution at a temperature of 80°C under reflux, and evaporate the solvent under reduced pressure to obtain a crude extract of Achills sylvestris whole plant;
[0043] b. Disperse the crude extract obtained in step a with water, sequentially add n-hexane, dichloromethane and n-butanol for extraction 4 times, combine the dichloromethane layers, and evaporate to dryness under reduced pressure to obtain a dichloromethane extract extract;
[0044] c. Separate the dichloromethane extract obtained in step b with a normal phase silica gel column, perform gradient elution with n-hexane-acetone with a volume ratio of 100:1-0:1, and analyze the fraction by silica gel thin layer chromatography , The same fractions were combined to obtain 9 components F1-F9; the component F4 was separated by Sephadex LH-20 gel column, and eluted with chloroform-methanol with a volume ratio of 1:1 to obtain ...
Embodiment 3
[0046] a. Take 5kg of the whole Chiba Achillea, pulverize and extract with 40L of absolute ethanol at room temperature, and evaporate the solvent under reduced pressure to obtain a crude extract of the whole Chiba Achillea;
[0047] b. Disperse the crude extract obtained in step a with water, sequentially add cyclohexane, ethyl acetate, and n-butanol for extraction 5 times, combine the ethyl acetate layers and evaporate to dryness under reduced pressure to obtain ethyl acetate extract extract;
[0048] c. Separate the ethyl acetate extract obtained in step b with a normal phase silica gel column, and perform gradient elution with cyclohexane-acetone with a volume ratio of 50:1-0:1, and the fractions are subjected to silica gel thin-layer chromatography Analyze and combine the same fractions to obtain 9 components F1-F9; separate component F4 through an ODS reverse phase column, eluting with a methanol-water solution gradient with a concentration of 40%-100%, and collect 60% methanol...
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