Preparation method of composite slow-release microcapsule preparation for treating ammonia nitrogen in aquaculture water
A technology of slow-release microcapsules and aquaculture water, which is applied in the field of biotechnology and microecological preparations, can solve the problems of poor viability, increase of ammonia nitrogen in water, and short action time, and achieve high nutritional value, good growth environment, and compact structure Effect
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Embodiment 1
[0041] (1) Activation culture: Bacillus cereus bacterial suspension, Bacillus subtilis bacterial suspension, and Bacillus licheniformis bacterial suspension are mixed at a bacterial count ratio of 1:1:1 and then placed in the activated medium to control the volume of the culture solution The filling volume is 15%, the activation culture temperature is 32 °C, the pH is 7.0, and the rotation speed is 160 r / min; after activating and culturing for 24 hours, the seed liquid is obtained; wherein the activation medium components are (g / L): glucose 5.0, peptone 5.0, Beef extract 2.0, NaCl 5.0.
[0042] (2) Fermentation culture: the seed liquid of step (1) was inoculated into the fermentation medium with a volume inoculation amount of 5%, the fermentation culture temperature was 32° C., the pH was 7.0, and the rotation speed was 160 r / min; The spores were collected and dried; the fermentation medium components were (g / L): glucose 5.0, peptone 5.0, beef extract 5.0, NaCl 3.0, MgSO4 7H2O...
Embodiment 2
[0046] (1) Activation culture: Bacillus cereus bacterial suspension, Bacillus subtilis bacterial suspension, and Bacillus licheniformis bacterial suspension are mixed at a bacterial count ratio of 1:1:2 and then placed in the activated medium to control the volume of the culture solution The filling volume is 20%, the activation culture temperature is 34°C, the pH is 7.2, and the rotation speed is 180r / min; after activating and culturing for 30 hours, the seed liquid is obtained; wherein the activation medium components are (g / L): glucose 8.0, peptone 8.0, Beef extract 4.0, NaCl 7.0.
[0047] (2) Fermentation culture: the seed liquid of step (1) was inoculated into the fermentation medium with a volume inoculation amount of 6%, the fermentation culture temperature was 35°C, the pH was 7.3, and the rotation speed was 180r / min; The spores were collected and dried; the fermentation medium components were (g / L): glucose 8.0, peptone 9.0, beef extract 7.0, NaCl 4.0, MgSO4 7H2O 1.0,...
Embodiment 3
[0051] (1) Activation culture: Bacillus cereus bacterial suspension, Bacillus subtilis bacterial suspension, and Bacillus licheniformis bacterial suspension are mixed at a bacterial count ratio of 1:2:3 and then placed in the activated medium to control the volume of the culture solution The filling volume is 25%, the activation culture temperature is 36 °C, the pH is 7.5, and the rotation speed is 200 r / min; after activating and culturing for 48 hours, the seed liquid is obtained; wherein the activation medium components are (g / L): glucose 10.0, peptone 10.0, Beef extract 5.0, NaCl 10.0.
[0052] (2) Fermentation culture: the seed liquid of step (1) was inoculated into the fermentation medium with an inoculation volume of 8%, the fermentation culture temperature was 37° C., the pH was 7.5, and the rotation speed was 200 r / min; The spores were collected and dried; the fermentation medium components were (g / L): glucose 10.0, peptone 10.0, beef extract 8.0, NaCl 5.0, MgSO4 7H2O ...
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