C-reactive protein preservation fluid, calibration product, quality control product and detection kit

A technology of reactive protein and protein protective agent, which is applied in the field of protein preservation, can solve problems such as wrong test results, waste, and can only be discarded, and achieve the effects of extending the validity period, reducing testing costs, and avoiding waste

Active Publication Date: 2019-05-21
SONOSCAPE MEDICAL CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, we must be very careful during the storage process, especially during the transportation process. Cold chain transportation must be guaranteed, and the temperature must be monitored at all times. A little carelessness will lead to the deterioration of the calibrator or quality control product, which will lead to wrong test results.
The C-reactive protein calibrator and quality control products of the current technology have high transportation costs, complicated packaging, time-consuming and labor-intensive, and once deteriorated, they can only be discarded, which is a serious waste
[0005] There are some p...

Method used

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  • C-reactive protein preservation fluid, calibration product, quality control product and detection kit
  • C-reactive protein preservation fluid, calibration product, quality control product and detection kit
  • C-reactive protein preservation fluid, calibration product, quality control product and detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Using 20mmoL / L, pH 8.0 Tris buffer, 0.9% NaCl, 15% sorbitol, 3% sucrose, 0.1% TritonX-405 and 0.1% KroVin400 as the basic formula A, adding a stabilizer to the basic formula A to prepare C-reactive protein preservation solution.

[0028] Add C-reactive protein to the above-prepared C-reactive protein preservation solution to prepare samples to be tested with protein concentrations of 5mg / L, 20mg / L, 80mg / L and 180mg / L, respectively numbered as samples 1, 2, 3 and 4 , to investigate the change of the measured value of C-reactive protein concentration in each sample stored at room temperature for 6 months and the original C-reactive protein concentration value of the sample to be tested. The experimental results are shown in Table 1.

[0029] Table 1 Stability of C-reactive protein in C-reactive protein preservation solution for 1 month, 2 months, 4 months and 6 months under normal temperature conditions

[0030]

[0031]

[0032] In this example, the effects of usi...

Embodiment 2

[0034] C-reactive protein preservation solution was prepared with 20mmoL / L, pH 8.0 Tris buffer, 0.9% NaCl, 15% sorbitol, 3% sucrose, 0.1% TritonX-405, 0.1% KroVin400 and 2% complex protein protectant HPD.

[0035] Contrast solution: CRP (II) standard solution produced by Nippon Seiken Co., Ltd.

[0036]Take the C-reactive protein preservation solution prepared above, and prepare C-reactive protein test samples 1, 2, 3, and 4 of 5 mg / L, 20 mg / L, 80 mg / L, and 180 mg / L according to the concentration range of the sample to be tested. Take the above-mentioned contrast solution to prepare a solution corresponding to the above-mentioned C-reactive protein concentration as a comparative example, and investigate the bottle-opening stability and long-term stability of the two groups of C-reactive protein solutions. The specific experimental operation is as follows: divide the two groups of C-reactive protein solutions into 8 parts, three of which were opened for 4 hours, then covered an...

Embodiment 3

[0044] Embodiment 3: the accuracy analysis of C-reactive protein calibrator

[0045] Take 20mmoL / L pH 8.0 Tris buffer, 0.9% NaCl, 15% sorbitol, 3% sucrose, 0.1% TritonX-405, 0.1% KroVin400 and 2% compound protein protectant HPD to prepare C-reactive protein preservation solution C-reactive protein preservation solution, adding C-reactive protein at a concentration required for calibration to the C-reactive protein preservation solution to prepare a C-reactive protein calibrator. Use the above-mentioned C-reactive protein calibrator to calibrate the detection system, test 100 clinical samples, and then calibrate the detection system with the CRP (II) standard solution produced by Japan Seiken Co., Ltd., and test the 100 samples. The two sets of results were compared, and the comparison results were as follows: figure 1 shown. Experimental results show that the C-reactive protein calibrator of the present invention has the same accuracy as the CRP (II) standard solution produc...

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Abstract

The invention discloses a C-reactive protein preservation fluid, a calibration product, a quality control product and a detection kit. The C-reactive protein preservation fluid comprises a buffer solution, 0.9-9.0 wt% of sodium chloride, 10-30 wt% of sorbitol, 0.5-5 wt% of sucrose, 0.05-0.2 wt% of a surfactant, 0.05-0.1 wt% of a preservative and 0.5-5 wt% of a protein protective agent. The C-reactive protein preserving fluid can prolong the shelf life of low-temperature preservation of C-reactive protein, the effect of normal-temperature preservation can be achieved through mutual cooperationof all the components, waste caused by improper low-temperature preservation can be avoided by using the C-reactive protein preserving fluid, and the detection cost is reduced.

Description

technical field [0001] The invention relates to the technical field of protein preservation, in particular to a C-reactive protein preservation solution, a calibration product, a quality control product and a detection kit. Background technique [0002] C-reactive protein (CRP) was discovered by Tillett and Francis in 1930 in the serum of patients with pneumonia, a non-specific bacterial polysaccharide component of pneumococcus (now called Streptococcus pneumoniae) C polysaccharide (C-polysacch aride, CPS) occurs Substances that precipitate reactions. In 1941, Macleod and Avery successively confirmed that this is a protein that appears during acute infection, and the flocculent precipitation reaction with C polysaccharides depends on Ca 2+ The presence. When tissue is damaged, inflamed or infected, CRP is induced by cytokines such as interleukin 6 (IL-6), and is mainly synthesized in the liver, which is an acute phase phase response protein. The concentration of CRP in th...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/96
Inventor 肖成勇刘兵白红霞
Owner SONOSCAPE MEDICAL CORP
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