C-reactive protein preservation fluid, calibration product, quality control product and detection kit

Abstract

The invention discloses a C-reactive protein preservation fluid, a calibration product, a quality control product and a detection kit. The C-reactive protein preservation fluid comprises a buffer solution, 0.9-9.0 wt% of sodium chloride, 10-30 wt% of sorbitol, 0.5-5 wt% of sucrose, 0.05-0.2 wt% of a surfactant, 0.05-0.1 wt% of a preservative and 0.5-5 wt% of a protein protective agent. The C-reactive protein preserving fluid can prolong the shelf life of low-temperature preservation of C-reactive protein, the effect of normal-temperature preservation can be achieved through mutual cooperationof all the components, waste caused by improper low-temperature preservation can be avoided by using the C-reactive protein preserving fluid, and the detection cost is reduced.

Description

technical field [0001] The invention relates to the technical field of protein preservation, in particular to a C-reactive protein preservation solution, a calibration product, a quality control product and a detection kit. Background technique [0002] C-reactive protein (CRP) was discovered by Tillett and Francis in 1930 in the serum of patients with pneumonia, a non-specific bacterial polysaccharide component of pneumococcus (now called Streptococcus pneumoniae) C polysaccharide (C-polysacch aride, CPS) occurs Substances that precipitate reactions. In 1941, Macleod and Avery successively confirmed that this is a protein that appears during acute infection, and the flocculent precipitation reaction with C polysaccharides depends on Ca 2+ The presence. When tissue is damaged, inflamed or infected, CRP is induced by cytokines such as interleukin 6 (IL-6), and is mainly synthesized in the liver, which is an acute phase phase response protein. The concentration of CRP in th...

AI Technical Summary

Problems solved by technology

Therefore, we must be very careful during the storage process, especially during the transportation process. Cold chain transportation must be guaranteed, and the temperature must be monitored at all times. A little carelessness will lead to the deterioration of the calibrator or quality control product, which will lead to wrong test results.

The C-reactive protein calibrator and quality control products of the current technology have high transportation costs, complicated packaging, time-consuming and labor-intensive, and once deteriorated, they can only be discarded, which is a serious waste

[0005] There are some preservation solutions used to maintain protein stability and activity in the prior art, but some are not suitable for the preservation of C-reactive protein reagents or have unsatisfactory preservation effects on C-reactive protein

For example, Chinese patent document CN 101324589 discloses a high-efficiency protein stabilizer obtained by chemical synthesis, which is used to maintain the stability of immunodiagnostic reagents. The aqueous solution prepared by the stabilizer is used to dilute protein and can be stored at 4°C for 10 days. The protein activity is above 80%, and the activity retention time is still not ideal

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