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A nano-co based 3 o 4 Double-amplified Zearalenone Impedance Sensor Mimicking Enzyme Catalysis

A technology of zearalenone and simulated enzymes, which is applied in the direction of instruments, scientific instruments, and electrochemical variables of materials, can solve the problems of poor stability and variability, and achieve high stability, good biocompatibility, and catalytic activity Good results

Inactive Publication Date: 2021-05-11
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to overcome the shortcomings of traditional biological enzymes such as variability, poor stability, and catalytic activity that is easily affected by the environment (pH, temperature, air pressure), etc., the inorganic metal oxide Co 3 o 4 As a new type of nano-enzyme material, it has broad prospects and important practical significance.

Method used

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  • A nano-co based <sub>3</sub> o <sub>4</sub> Double-amplified Zearalenone Impedance Sensor Mimicking Enzyme Catalysis
  • A nano-co based <sub>3</sub> o <sub>4</sub> Double-amplified Zearalenone Impedance Sensor Mimicking Enzyme Catalysis
  • A nano-co based <sub>3</sub> o <sub>4</sub> Double-amplified Zearalenone Impedance Sensor Mimicking Enzyme Catalysis

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Experimental program
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Effect test

Embodiment 1

[0035] 1. A nano-Co based 3 o 4 A method for preparing a zearalenone impedance sensor with signal double amplification simulating enzyme catalysis is characterized in that the preparation method mainly includes the following steps:

[0036] (1) The glassy carbon electrode is mechanically polished on wet suede with 50nm and 30nm alumina powder in turn, and then rinsed with double distilled water and absolute ethanol for several times until the electrode surface is clean and bright.

[0037] (2) Pipette 5 μL of hyaluronic acid-functionalized TiO with a concentration of 3 mg / L 2 mesogenic solution (TiO 2 @Hya) is added dropwise to the surface of a clean glassy carbon electrode, dried in an infrared light box, taken out, and cooled to room temperature, thus preparing TiO 2 Glassy carbon electrode modified by @Hya;

[0038] (3) Add 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysulfosuccinimide sodium salt ( NHS) mixed solution on the surface of ...

Embodiment 2

[0045] The hyaluronic acid functionalized TiO used in the above-mentioned embodiment 1 2 The mesogenic solution was prepared by the following method:

[0046] 1) Dissolve 0.5g of sodium dodecylbenzenesulfonate (SDBS) in 25 mL of 2.2 mol / L HNO 3 In the solution, 500 µL of isopropyl titanate (TIP) was added after continuous stirring and shaking for tens of minutes, and then the above mixture was kept shaking at 48 °C for 24 hours, and then the mixture was washed with deionized water and absolute ethanol for several times. Once, dry overnight at 60°C. After drying, it was calcined at 400 °C for 60 min to fully remove residual organic matter and obtain the final product. The resulting product was dispersed in deionized water and stored in a 4 °C refrigerator until further use.

[0047] 2) TiO functionalized with hyaluronic acid 2 The mesogenic solution was prepared by mixing 500 μL of 5 mg / mL hyaluronic acid solution and 500 μL of 5 mg / mL TiO 2 The mesogens were mixed and sha...

Embodiment 3

[0049] The k-Co used in the above-mentioned embodiment 1 3 o 4 Prepared by the following method:

[0050] 1) Dissolve the addition polymer of 6g of polypropylene glycol and ethylene oxide in 217g of deionized water and 11.8g of 35% hydrochloric acid mixed solution, add 6g of butanol to the above solution at 35°C, and keep stirring for 1h Finally, under the condition of 35° C., 12.9 g of polytetramethoxysilane was added, followed by continuous stirring for 24 h. Subsequently, the above mixture was heated to 100°C and kept for 24h, and the solid product obtained after the hydrothermal treatment was filtered and dried at 100°C. The obtained product was extracted in a mixture of ethanol and hydrochloric acid, calcined at 550°C, and cooled to obtain a KIT-6 template.

[0051] 2) 0.776 g of cobalt nitrate hexahydrate (Co(NO 3 ) 2 ·6H 2 O) Dissolve in 5 mL of absolute ethanol solution as a precursor solution, add 0.2 g of the KIT-6 template prepared in step (1) after continuous...

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Abstract

The invention discloses a nano-Co based 3 o 4 A double-amplified impedance sensor for zearalenone mimicking enzyme catalysis. First, TiO functionalized with hyaluronic acid 2 As a sensing platform, mesogens utilize hyaluronic acid to capture a large number of primary antibodies (Ab 1 ), and then sequentially recognize the upper zearalenone antigen on the sensing interface, and Co 3 o 4 Labeled secondary antibody (Ab 2 ). The resulting immune complexes impede electron transfer and increase the electrochemical impedance signal. Nano Co 3 o 4 As an inorganic metal oxide, it has good properties of peroxide imitating enzymes, better stability, high catalytic activity, catalytic oxidation produces a large amount of biological precipitation on the surface of the test electrode, which further amplifies the detection signal. Based on the double amplification of the detection signal, an impedance sensor with high sensitivity, good stability and low detection limit was constructed, and the ultrasensitive detection of zearalenone was realized.

Description

technical field [0001] The invention belongs to the technical field of novel inorganic functional materials and biosensing detection, in particular to a nano-Co based 3 o 4 Preparation method and application of zearalenone impedance sensor with signal double amplification catalyzed by simulated enzyme. Background technique [0002] Zearalenone (ZEA) is a widely distributed miscellaneous mycotoxin, usually found in moldy and spoiled corn, rice and other grains, mainly the fungus of various Fusarium species, such as yellow Fusarium (f.culmorum), Fusarium seminaked (f.ceriisand), Fusarium equiseti (f.equiseti) and other strains. Studies have shown that ZEA is very harmful to the health of humans and animals. If food containing a small amount or even a trace of ZEA is eaten by mistake, it will often cause serious diseases, such as ovarian dysfunction, embryonic dysplasia and abnormal reproductive organ function. Therefore, it is of great significance to develop a detection me...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327G01N33/577
Inventor 戴宏陈妍洁王佳妮衣欢高利红韩晴郑祥钦
Owner FUJIAN NORMAL UNIV
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