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Preparation method of petroleum-degrading microbial agent

A technology of petroleum-degrading bacteria and preparation steps, which is applied in the field of petroleum pollution restoration, can solve the problems of limited storage time of petroleum-degrading bacteria, achieve optimal immobilization effect, strong adsorption capacity, and increase contact effect

Inactive Publication Date: 2019-06-14
陈傲
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Technical problem to be solved by the present invention: Aiming at the problem of limited storage time of existing petroleum degrading bacterial agents, a preparation method of petroleum degrading bacterial agents is provided

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0028]Crush fresh pomelo peels and dry them in an oven at 90°C to constant weight to obtain crushed pomelo peels. Mix the crushed pomelo peels with activated carbon, perform pyrolysis treatment, cool to room temperature, grind and pass through a 30-mesh sieve , the mixture is obtained; the oil-degrading bacteria are inserted into the seed medium, cultivated, and the seed liquid is obtained, and the solid medium is added to the seed liquid, and the culture is processed, and the bacterial liquid is obtained; the mixture and the bacterial liquid are mixed, and vacuumized treatment and filtration to obtain filter residue; mix polylactic acid fiber and ionic liquid, stir and cool to room temperature to obtain a mixed solution, add filter residue to the mixed solution, stir and spray dry, cool to room temperature to obtain petroleum degradation Bacteria. Mix crushed pomelo peels and activated carbon, and perform pyrolysis treatment steps: mix crushed grapefruit peels and activated c...

example 2

[0030] Crush the fresh pomelo peel and dry it in an oven with a temperature of 93°C to constant weight to obtain the crushed pomelo peel, mix the crushed pomelo peel with activated carbon, perform pyrolysis treatment, cool to room temperature, grind and pass through a 39-mesh sieve , the mixture is obtained; the oil-degrading bacteria are inserted into the seed medium, cultivated, and the seed liquid is obtained, and the solid medium is added to the seed liquid, and the culture is processed, and the bacterial liquid is obtained; the mixture and the bacterial liquid are mixed, and vacuumized treatment and filtration to obtain filter residue; mix polylactic acid fiber and ionic liquid, stir and cool to room temperature to obtain a mixed solution, add filter residue to the mixed solution, stir and spray dry, cool to room temperature to obtain petroleum degradation Bacteria. Mix crushed pomelo peels and activated carbon, and perform pyrolysis treatment steps: mix crushed grapefrui...

example 3

[0032] Crush the fresh pomelo peel and dry it in an oven with a temperature of 96°C to constant weight to obtain the crushed pomelo peel, mix the crushed pomelo peel with activated carbon, perform pyrolysis treatment, cool to room temperature, grind and pass through a 45-mesh sieve , the mixture is obtained; the oil-degrading bacteria are inserted into the seed medium, cultivated, and the seed liquid is obtained, and the solid medium is added to the seed liquid, and the culture is processed, and the bacterial liquid is obtained; the mixture and the bacterial liquid are mixed, and vacuumized treatment and filtration to obtain filter residue; mix polylactic acid fiber and ionic liquid, stir and cool to room temperature to obtain a mixed solution, add filter residue to the mixed solution, stir and spray dry, cool to room temperature to obtain petroleum degradation Bacteria. Mix crushed pomelo peels and activated carbon, and perform pyrolysis treatment steps: mix crushed grapefrui...

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Abstract

The invention relates to a preparation method of a petroleum-degrading microbial agent and belongs to the technical field of petroleum pollution remediation. According to the method, grapefruit peel and activated carbon are subjected to pyrolysis treatment to serve as a microbial agent carrier, a large amount of cellulose is contained in a white flocculent layer in the grapefruit peel, the grapefruit peel also has the characteristics of multiple pores and a large pore diameter and has a compact structure, the network structure is not obvious, oil products are adsorbed to a certain extent, thethree-dimensional network structure of the pyrolyzed grapefruit peel is more obvious, large and small pores are communicated in a staggered manner, and rapid oil absorption is facilitated, and the pore diameter is obviously increased compared with that before pyrolysis, so that the improvement of oil absorption is facilitated; due to the fact that an artificial carrier has the advantages of controllable specific surface area, pore size distribution, good mass transfer performance and the like, the artificial carrier can be better matched with specific microbes and complex pollutants so as to obtain better immobilization effect and repair performance; biochar has strong petroleum adsorption capacity, achieves the enrichment and degradation integration of pollutants and is beneficial to theimprovement of the soil remediation effect.

Description

technical field [0001] The invention relates to a preparation method of a petroleum degrading bacterial agent, belonging to the technical field of petroleum pollution restoration. Background technique [0002] With the increasing amount of oil extraction, a large amount of oil enters the environment during extraction, transportation, storage, and accidental leakage, seriously polluting soil, groundwater, rivers, and oceans. The use of microorganisms to remediate oil-contaminated soil is an emerging technology that cleans the environment with low investment, high benefits, easy application, and great development potential. During the production, processing and use of petroleum, due to abnormal operation, accidents and maintenance, etc., there will be spillage and discharge of petroleum hydrocarbons, causing petroleum pollution to the environment, and it is imminent to repair the pollution. Microbial remediation technology is an efficient, safe, and low-cost oil pollution rem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/14C12N11/08C12N11/04B09C1/10C12R1/125C12R1/38
Inventor 陈傲
Owner 陈傲
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