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Doxorubicin fluorescence detection method based on carboxylated chitosan/dithiothreitol-gold nanoclusters

A technology of gold nanoclusters and dithiothreitol, which is applied in the field of analytical chemistry and nanometers, can solve the problems of complex pretreatment process, long response time, expensive instruments, etc., and achieve high test sensitivity, short detection time and simple method Effect

Inactive Publication Date: 2019-06-14
FUJIAN MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods have certain defects, such as expensive instruments and toxic reagents, complicated pretreatment process, poor reproducibility, and long response time, etc.

Method used

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  • Doxorubicin fluorescence detection method based on carboxylated chitosan/dithiothreitol-gold nanoclusters
  • Doxorubicin fluorescence detection method based on carboxylated chitosan/dithiothreitol-gold nanoclusters
  • Doxorubicin fluorescence detection method based on carboxylated chitosan/dithiothreitol-gold nanoclusters

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1 mL of 0.4 mol / L sodium hydroxide solution and 1.6 mL of 20 mg / mL chloroauric acid were mixed in advance, and then 7.4 mL of 50 mg / mL carboxylated chitosan solution and 10 mL of 0.1 mol / L dithiothreitol solution, shake well and place in a water bath at 37°C for constant temperature reaction for 8 hours to obtain carboxylated chitosan / dithiothreitol-gold nanoclusters. The reacted gold nanoclusters were dialyzed in double distilled water for 24 hours with a dialysis bag with a molecular weight cut off of 3500 to obtain purified carboxylated chitosan / dithiothreitol-gold nanoclusters. The resulting carboxylated chitosan / dithiothreitol-gold nanoclusters are strongly fluorescent with excitation and emission maxima at 285 nm and 645 nm, respectively (see figure 1 ).

Embodiment 2

[0037] Add 0.1 mL of the gold nanocluster solution prepared in Example 1 into 1.9 mL of phosphate buffer (20 mmol / L, pH=6.0) containing 5 μmol / L doxorubicin, and mix well. Depend on figure 2 It can be seen that after adding doxorubicin, the emission intensity of the system at 645 nm is significantly reduced (excitation wavelength is 285 nm).

Embodiment 3

[0039] Add 0.1 mL of the gold nanocluster solution prepared in Example 1 into 1.9 mL of phosphate buffer (20 mmol / L, pH=6.0) containing 5 μmol / L doxorubicin, and mix well. At the same time, set a group of blank control group without doxorubicin ( image 3 in A). After the reaction was completed, the color change was observed under ultraviolet light. Such as image 3 As shown, after adding doxorubicin, the red fluorescence of gold nanoclusters was almost completely quenched ( image 3 in B).

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Abstract

The invention discloses a doxorubicin fluorescence detection method based on carboxylated chitosan / dithiothreitol-gold nanoclusters. The doxorubicin fluorescence detection method based on the carboxylated chitosan / dithiothreitol-gold nanoclusters uses the effect that strong photoinduced electron transfer occurs after the specificity interaction of the gold nanoclusters and doxorubicin to cause thequenching of the fluorescence of the gold nanoclusters, thereby realizing the determination of doxorubicin. The linear range of doxorubicin determination is 0.05[Mu]mol / L to 2[Mu]mol / L, and the minimum detection limit is 0.005[Mu]mol / L. The gold nanoclusters used in the new detection method are simple and easy to prepare, and the new detection method has the advantages of simple operation, shortdetection time, high sensitivity and strong specificity, and is easy to popularize and use.

Description

technical field [0001] The present invention is based on carboxylated chitosan / dithiothreitol-gold nano-cluster fluorescent material, provides a novel rapid and ultra-sensitive detection method for doxorubicin (doxorubicin), and belongs to the field of analytical chemistry and nanotechnology . Background technique [0002] As we all know, doxorubicin is an anthraquinone compound, which is widely used in the treatment of leukemia and breast cancer. Although doxorubicin has been proven to be an effective drug, its therapeutic index is low, and long-term use or low doses can cause some serious side effects, including bone marrow suppression and cardiotoxicity. [0003] Many analytical methods, including high performance liquid chromatography, capillary electrophoresis, mass spectrometry, electrochemical methods, etc., have been reported for the detection of doxorubicin. However, these methods have certain defects, such as expensive instruments and toxic reagents, complicated ...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 陈伟邓豪华黄开源彭花萍何宏星
Owner FUJIAN MEDICAL UNIV
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