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Dry chemical reagent tablet for quantitatively measuring activity of alanine aminotransferase and preparation method of dry chemical reagent tablet

A technology for the quantitative determination of alanine amino groups, which is applied in the field of dry chemical reagent tablets for quantitative determination of alanine aminotransferase activity and its preparation, can solve the problem of great health hazards for preparation personnel, high requirements for materials and equipment, and complex production processes and other problems, to achieve the effect of wide application range, good repeatability, and simple preparation process

Inactive Publication Date: 2019-06-21
吉林省汇酉生物技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above-mentioned dry chemical reagent sheets can be quantitatively detected, organic reagents are required for the distribution layer or diffusion layer, which requires high materials and equipment, complicated production technology, and is extremely harmful to the health of the preparation personnel and pollutes the environment.

Method used

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  • Dry chemical reagent tablet for quantitatively measuring activity of alanine aminotransferase and preparation method of dry chemical reagent tablet
  • Dry chemical reagent tablet for quantitatively measuring activity of alanine aminotransferase and preparation method of dry chemical reagent tablet
  • Dry chemical reagent tablet for quantitatively measuring activity of alanine aminotransferase and preparation method of dry chemical reagent tablet

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Embodiment 1: the preparation method of alanine aminotransferase dry chemical reagent sheet of the present invention

[0067] The transparent support layer is made of polyethylene terephthalate, with a thickness of 0.188 mm, a length of 30 cm, and a width of 30 cm. The surface is treated by ultraviolet irradiation, the irradiation distance is 5cm, and the irradiation time is 60 minutes.

[0068] The formula of chromogenic solution reagent is as follows:

[0069]

[0070] The formula of reaction liquid reagent is as follows:

[0071]

[0072] The color-developing layer is coated on the ultraviolet-treated polyethylene terephthalate support layer, and the reagent layer is continuously coated on the color-developing layer, and put into a drying box for drying at 30°C for 20 minutes.

[0073] The formula of the diffusion liquid reagent is as follows:

[0074]

[0075]

[0076] Spray the diffusion liquid evenly on the reagent layer, and put it in a drying oven...

Embodiment 2

[0078] Embodiment 2: the accuracy analysis of the method of the present invention

[0079] Test samples: blood samples from 20 medical examiners;

[0080] Comparative detection method: use commercially available alanine aminotransferase detection kit (alanine substrate method) 2:1 liquid reagent to detect on Hitachi 7080 automatic biochemical analyzer, and add 2.5 μL sample to the automatic biochemical analyzer , Reagent 1 160 μL, Reagent 2 80 μL, react at 37°C for 6.5 min to 8 min to measure the rate of change of absorbance at 340 nm, and calculate the activity of alanine aminotransferase in the sample according to the standard curve.

[0081] 20 samples were measured respectively with the detection method of Example 1 and the comparative detection method, and the detection results are shown in Table 1.

[0082] Table 1 analysis results

[0083]

[0084] The results show that the R calculated according to the test results 2 The value is 0.9999, which is greater than 0.9...

Embodiment 3

[0085] Embodiment 3: the precision analysis of the method of the present invention

[0086] Test sample: any serum sample;

[0087] Using the detection method of Example 1, the same sample to be tested was repeatedly detected 10 times, and the detection results are shown in Table 2. Table 2 detects sample alanine aminotransferase activity (10 times) and standard deviation rate (variation coefficient)

[0088]

[0089] The results show that the standard deviation rate is 1.89%, which is less than 10% of the standard requirement, indicating that the method of the present invention has high precision.

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Abstract

The invention belongs to the technical field of clinical in vitro diagnostic reagents and relates to a dry chemical reagent tablet for quantitatively measuring the activity of alanine aminotransferaseand a preparation method of the dry chemical reagent tablet. The dry chemical reagent tablet comprises four laminations which are respectively a support layer, a color developing layer, a reagent layer and a diffusion layer distributed sequentially; reagents in the reagent layer include a buffer system, a hydrophilic colloid, a pyruvate oxidase, peroxidase, thiamine pyrophosphate, a chelating agent, an activator and a surfactant; and reagents in the color developing layer include a chromogen, a hydrophilic colloid, and a surfactant. The dry chemical reagent tablet and the preparation method thereof of the invention have the advantages of simple operation process, high stability, high good repeatability and high accuracy of detection results, quantitative detection, wide linear range, simple and convenient preparation process, easiness in operation, low risk and low pollution. According to the dry chemical reagent tablet for quantitatively measuring the activity of the alanine aminotransferase and the preparation method of the dry chemical reagent tablet of the invention, the reagents do not need to be prepared, and samples can be directly added dropwise; the reagent tablet is dryand is free of moisture; and the diffusion layer has a filtering function, so that hemolysis, bilirubin and ascorbic acid samples exert no significant interference on measurement results.

Description

technical field [0001] The invention relates to the technical field of clinical in vitro diagnostic reagents, in particular to a dry chemical reagent sheet for quantitatively measuring alanine aminotransferase activity and a preparation method thereof. Background technique [0002] Alanine aminotransferase (ALT) is an enzyme involved in human protein metabolism, which can accelerate the conversion of alanine amino group into glutamic acid in the body. Under normal circumstances, ALT is mainly present in tissue cells such as liver, kidney, heart muscle, skeletal muscle, pancreas, spleen, lung, and red blood cells, and also in normal body fluids such as plasma, bile, cerebrospinal fluid, and saliva, especially in liver. The content is the highest, about 200 times that of plasma. But it does not exist in the urine, except for the occurrence of kidney damage or disease. The activity of ALT in serum is very low. When some reasons increase the permeability of cell membrane or de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31
Inventor 王菁菁崔建华董理
Owner 吉林省汇酉生物技术股份有限公司
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