A method for detecting formaldehyde content in cells by Hanch reaction using water-soluble polymers
A formaldehyde content, water-soluble polymer technology, applied in the field of materials, can solve the problems of high toxicity of acetylacetone, weak fluorescent signal, and difficult to store
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Embodiment 1
[0039] 1. Preparation of water-soluble diketone structure polymer
[0040] according to figure 1 The preparation route of mixing a certain proportion of polyethylene glycol methyl methacrylate (molecular weight is 950) and acetoacetate ethylene glycol methacrylate, the ratio of its substance amount is 1:1.
[0041] Add the mixed polyethylene glycol methyl methacrylate and ethylene glycol methacrylate acetoacetate into the polymerization tube, add the initiator azobisisoheptanonitrile, add the solvent dimethylformamide, and seal it with a rubber stopper .
[0042] Nitrogen was blown for 30 minutes, and the air inside the polymerization tube was purged. Stir and heat in an oil bath at 70°C for 8 hours.
[0043] The polymerization stock solution was added to diethyl ether to precipitate, and centrifuged (10000 rpm, 10 minutes) to obtain a precipitated solid.
[0044] The obtained polymer solid was cooled with liquid nitrogen, and cooled and sucked dry to obtain a water-solubl...
Embodiment 2
[0049] Embodiment 2, fluorescence spectrum test experiment
[0050] 1) According to the preparation method of Example 1, after the polymer is obtained, a formaldehyde detection solution is formulated with a certain concentration and ammonium salt. A certain quality of acetylacetone and ammonium salt is formulated into a traditional formaldehyde detection solution. The ratio of the amount of acetylacetone to the ammonium salt is 1:2.5, and the ammonium salt is ammonium acetate.
[0051] 2) A trace amount of formaldehyde solution (concentration of 100 μmol / L) was added, and the excitation wavelength was 360 nanometers, and the sensitivity and timeliness of the two were tested by fluorescence spectroscopy. Such as image 3 ,4.
[0052] Depend on image 3,4 It can be seen that: comparatively speaking, the detection sensitivity of the polymer-based detection solution is higher than that of the traditional acetylacetone, and it has a stronger fluorescence intensity.
Embodiment 3
[0053] Embodiment 3, detection limit test experiment
[0054] Get the water-soluble polymer and ammonium acetate that make in example 1 and be made into formaldehyde detection solution (macromolecule concentration is 20mg / mL; The concentration of ammonium acetate is 2mg / mL), contrast with traditional acetylacetone and ammonium acetate detection solution ( The polymer concentration is 1mg / mL; the concentration of ammonium acetate is 2mg / mL), react with different concentrations of formaldehyde solution respectively, detect the fluorescence photometric value measured by the porous microplate reader, and measure it according to the linear relationship between the fluorescence photometric value and the formaldehyde concentration detection limit. The result is as Figure 5 . Depend on Figure 5 a, b shows that: comparatively speaking, the detection effect of formaldehyde based on polymer-based detection solution can achieve the detection effect similar to that of traditional acet...
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