Spectrophotometric method for detecting hydrogen peroxide content based on enzyme-catalyzed oxidation coloration of potassium iodide

Abstract

The invention discloses a spectrophotometric method for detecting hydrogen peroxide content based on enzyme-catalyzed oxidation coloration of potassium iodide. The spectrophotometric method of the invention uses catalase as a catalyst and potassium iodide as an indicator, and can rapidly detect hydrogen peroxide content in a solution. Compared with the existing measuring method, the hydrogen peroxide is catalyzed to quickly oxidize potassium iodide to generate elemental iodine by utilizing the catalase, the elemental iodine further reacts with excessive iodine ions in the solution to generateiodine trianions for measuring the hydrogen peroxide content, namely, the catalase reacts with the hydrogen peroxide quickly at first to generate an intermediate product in a reaction solution containing catalase, hydrogen peroxide and potassium iodide, then the potassium iodide reacts with the generated intermediate product to generate elemental iodine, the elemental iodine reacts with the excessive iodine ions to generate iodine trianions, and the hydrogen peroxide concentration in ater is measured based on an absorbance value of the iodine trianions at a specific absorption wavelength.

Description

technical field [0001] The invention belongs to the technical field of hydrogen peroxide detection, and in particular relates to a spectrophotometric method for detecting hydrogen peroxide content based on enzyme-catalyzed potassium iodide oxidation and color development. Background technique [0002] Due to its strong oxidizing ability and environmental protection features, hydrogen peroxide is widely used as oxidant, disinfectant, sterilant and bleaching agent in environmental protection, daily chemical, food, medical and printing and dyeing industries. In addition, hydrogen peroxide can also be activated by transition metal ions, metal oxides, microwave radiation, and ultraviolet radiation to generate hydroxyl radicals with stronger oxidizing ability (E 0 =1.8-2.7V), is widely used in the field of environmental protection, especially in the field of water treatment to remove refractory organic pollutants and inactivate pathogenic microorganisms. In the process of using h...

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Problems solved by technology

Titration methods mainly include iodometric method and potassium permanganate method. This method has high sensitivity and accurate measurement results, but there are also shortcomings such as complicated operation and many interference factors.

Chromatography has the advantages of strong specificity, high accuracy, and strong anti-interference ability, such as patent ZL 201310745895.7. However, chromatography requires the use of expensive chromatographs, resulting in high cost of determination

Chemiluminescence method has the advantages of high sensitivity, low detection limit, and accurate measurement results, but it also has disadvantages such as long measurement time and high measurement cost.

Ultraviolet-visible spectrophotometry has reliable results, fast measurement speed, and relatively simple operation, such as patents ZL 201310745891.9 and ZL 201410034574.0, but there are also shortcomings such as long reaction time, toxic reaction reagents and easy to cause environmental pollution. The test paper colorimetric method It has the advantages of simple operation and fast measurement speed, such as patent ZL201310337923.1, but there are also disadvantages such as high cost of test paper preparation, poor accuracy of measurement results, and low sensitivity

The measurement results of the amperometric method are accurate and reliable, and the sensitivity is high, such as the patent ZL 200780034519.X, but there are also shortcomings such as high measurement results, long measurement time, and poor anti-interference ability

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