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Protein culture medium for fast propagating biological bacteria

A medium and biological bacteria technology, applied in the directions of microorganisms, microorganisms, biochemical equipment and methods, etc., can solve the problems of slow growth of microorganisms, unstable chemical composition of medium, and prolonged duration, so as to achieve long-term nutrient retention and rapid promotion. Effects of reproduction, metabolism, and nutritional balance

Inactive Publication Date: 2019-07-30
天适立农生态农业发展(广州)股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing medium is divided into natural medium, synthetic medium and semi-synthetic medium. Natural medium is made of natural substances. The chemical composition of this type of medium is not constant and difficult to determine. The uncertainty of medium composition It is easy to lead to the uncontrollability of the cultivation process. The synthetic medium is made of substances with completely known components. The chemical composition of this medium is clear, repeatable, and easy to industrialize. However, microorganisms in this type of medium The growth in medium is relatively slow, and semi-synthetic medium is based on the addition of natural substances on the basis of synthetic medium. Although compared with synthetic medium, the cultivation speed has been improved to a certain extent, but the cultivation speed is still slow and cannot meet the requirements The demand for rapid production and the slow growth of microorganisms lead to more time spent on microbial reproduction, prolonging the duration of the entire process of microbial cultivation and subsequent production and processing

Method used

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  • Protein culture medium for fast propagating biological bacteria

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Effect test

Embodiment 1

[0029] A protein culture medium for rapidly multiplying biological bacteria. The components of the protein culture medium and the parts by weight of each component are: 0.1 parts of growth factor, 20 parts of coagulant, 18 parts of protein additive, 0.5 part of pH regulator, trace amount 1.3 parts of elements, 9 parts of sugars, 30 parts of water. The medium is also added with complex decomposing enzymes: 3 parts of acetyl coenzyme A, 1 part of β-galactosidase, 8 parts of cellulase, 2 parts of chymotrypsin, 6 parts of subtilisin, and 1 part of leucyl aminopeptidase , 4 parts of xylan decomposing enzyme, 0.5 part of chitosan decomposing enzyme, 3 parts of pectin hydrolase, 7 parts of mannan decomposing enzyme.

[0030] The components of the growth factor and the parts by weight of each component are: 3 parts of bovine serum, 0.5 part of insulin, 0.7 part of sterol, 1 part of porphyrin, 0.01 part of fibroblast growth factor, 3 parts of rosehip oil, Cut melon extract 10 parts. ...

Embodiment 2

[0038] A protein culture medium for rapidly multiplying biological bacteria, the components of the protein culture medium and the parts by weight of each component are: 0.3 parts of growth factor, 25 parts of coagulant, 28 parts of protein additive, 1.5 parts of pH regulator, trace 2.9 parts of elements, 15 parts of sugars, 40 parts of water. The medium is also added with complex decomposing enzymes: 7 parts of acetyl coenzyme A, 6 parts of β-galactosidase, 12 parts of cellulase, 7 parts of chymotrypsin, 9 parts of subtilisin, and 3 parts of leucyl aminopeptidase , 8 parts of xylan decomposing enzyme, 2.5 parts of chitosan decomposing enzyme, 8 parts of pectin hydrolase, 11 parts of mannan decomposing enzyme.

[0039] The components of the growth factor and the parts by weight of each component are: 7 parts of bovine serum, 1.7 parts of insulin, 1.3 parts of sterol, 2 parts of porphyrin, 0.5 part of fibroblast growth factor, 4 parts of rosehip oil, Cut melon extract 15 parts....

Embodiment 3

[0048] A protein medium for rapidly multiplying organisms, the components of the protein medium and the parts by weight of each component are: 0.2 parts of growth factor, 22 parts of coagulant, 25 parts of protein additive, 1.1 parts of pH regulator, trace 2.4 parts of elements, 13 parts of sugars, 35 parts of water. The medium is also added with complex decomposing enzymes: 5 parts of acetyl coenzyme A, 5 parts of β-galactosidase, 10 parts of cellulase, 5 parts of chymotrypsin, 7 parts of subtilisin, and 2 parts of leucyl aminopeptidase , 6 parts of xylan decomposing enzyme, 2.2 parts of chitosan decomposing enzyme, 4 parts of pectin hydrolase, 10 parts of mannan decomposing enzyme.

[0049] The components of the growth factor and the parts by weight of each component are: 5 parts of bovine serum, 1.3 parts of insulin, 1.1 parts of sterol, 1.5 parts of porphyrin, 0.04 part of fibroblast growth factor, 3.5 parts of rosehip oil, Cut melon extract 12 parts.

[0050] The compon...

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Abstract

The invention relates to the field of microculture, in particular to a protein culture medium for fast propagating biological bacteria. The protein culture medium is prepared from, by weight, 0.1-0.3part of growth factors, 20-25 parts of a coagulator, 18-28 parts of a protein additive, 0.5-1.5 parts of a PH modifier, 1.3-2.9 parts of microelements, 9-15 parts of saccharide and 30-40 parts of water. According to the culture medium, absorption of microorganisms to nutrient substances and conduction of related metabolic processes can be promoted, and stable growth and propagation of microorganisms can be stimulated. In addition, by adding vitamin, the protein additive and thelike, enough energy can be provided for the microorganisms to take, and fast propagation and metabolism of the microorganisms are promoted. Compared with a culture medium in the prior art, the culture medium is high in microorganism culture speed and microorganism propagating speed, balanced in nutrition and long innutrition holding time.

Description

[0001] Technical field: [0002] The invention relates to the field of microorganism culture, in particular to a culture medium for fast-propagating organism bacterial protein. [0003] Background technique: [0004] Culture medium is artificially prepared nutrients for the growth and maintenance of microorganisms, plant tissues and animal tissues, and generally contains carbohydrates, nitrogenous substances, inorganic salts (including trace elements), vitamins and water. Different media can add some compounds that cannot be synthesized by themselves, that is, growth factors, according to actual needs. Some microorganisms, such as autotrophs, do not require a carbon source, so the above is only general. The existing medium is divided into natural medium, synthetic medium and semi-synthetic medium. Natural medium is made of natural substances. The chemical composition of this type of medium is not constant and difficult to determine. The uncertainty of medium composition It is...

Claims

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Application Information

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IPC IPC(8): C12N1/00
CPCC12N1/00
Inventor 刘友梅
Owner 天适立农生态农业发展(广州)股份有限公司
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