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A dry film detection reagent card for quantitative detection of hydrogen peroxide concentration and preparation method thereof

A technology of hydrogen peroxide concentration and detection reagent, which is applied in the direction of material analysis by observing the influence on chemical indicators, and analysis by chemical reaction of materials, etc. Limiting the application fields of dry slice detection reagent cards, etc., to achieve the effects of storage conditions that are not easy to inactivate, reduce the detection limit, and have a wide range of storage conditions

Active Publication Date: 2022-03-22
CHANGCHUN HUILI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many reagents in analytical tests that cannot be mixed in advance, and complex test solutions need to be pretreated, which limits the application field of dry film detection reagent cards

Method used

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  • A dry film detection reagent card for quantitative detection of hydrogen peroxide concentration and preparation method thereof
  • A dry film detection reagent card for quantitative detection of hydrogen peroxide concentration and preparation method thereof
  • A dry film detection reagent card for quantitative detection of hydrogen peroxide concentration and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1: Method for preparing the hydrogen peroxide dry sheet detection kit according to the present invention

[0063] The transparent support layer material is made of polyethylene terephthalate, and the thickness is 0.20 mm. The surface is coated with a bottom layer.

[0064] The formulation of the developing liquid reagent is as follows:

[0065]

[0066] The formulation of the diffusion liquid reagent is as follows:

[0067]

[0068]

[0069] The developing layer was coated on a coated underlying polyethylene terephthalate support layer, placed in a drying tank, dried at 60 ° C for 3 minutes.

[0070] Spray the diffusion liquid onto the reagent layer, and placed in a drying tank. Use a tailor to cut it into a tip piece of 15mm × 15 mm size, and the seal is stored.

[0071] Detection method: 10 μl of test samples were added dropwise to the reagent sheet, and after 5 minutes, the measurement was measured with a reflective photometer, and the concentration of hydr...

Embodiment 2

[0099] Example 2: Method for preparing the hydrogen peroxide dry sheet detection kick of the present invention

[0100] The transparent support layer material is made of polyethylene terephthalate, with a thickness of 0.15 mm. The surface is coated with a bottom layer.

[0101] The formulation of the developing liquid reagent is as follows:

[0102]

[0103] The formulation of the diffusion liquid reagent is as follows:

[0104]

[0105] The developing layer was coated on a coated underlying polyethylene terephthalate support layer, placed in a drying tank, 40 ° C for 5 minutes.

[0106] Spray the diffusion liquid onto the reagent layer, and placed in a drying tank. Use a tailor to cut it into a 10mm × 10 mm sized test strip, sealed.

[0107] Detection method: 15 μl of test sample was added dropwise to the reagent sheet, and after 5 minutes, the measurement was measured with a reflective photometer, and the concentration of hydrogen peroxide in the sample was calculated accord...

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Abstract

The invention relates to the technical field of biochemical detection, in particular to a dry film detection reagent card for quantitative detection of hydrogen peroxide concentration and a preparation method thereof. The dry film detection reagent card consists of three laminated layers, which are the support layer, the chromogenic layer and the diffusion layer in sequence; the reagents in the chromogenic layer include hydrophilic colloid, surfactant, chromogen A, chromogen B, chromogen C. The reagents in the diffusion layer include hydrophilic polymers, surfactants, particulate matter, and hypoheme hexapeptide. The dry film detection reagent card for quantitatively detecting the hydrogen peroxide concentration of the present invention can quantitatively detect the residual amount of hydrogen peroxide in food; the dry film detection reagent card comprises three laminated layers, which are a support layer, a color development layer and a diffusion layer in sequence; The color development system adopts a two-color primary system, which not only improves the linear range of detection, but also reduces the detection limit of the dry film detection reagent card; subheme hexapeptide is used instead of peroxidase, so that the detection card does not need to add cofactors, Cost reduction, wide storage conditions and not easy to inactivate.

Description

Technical field [0001] The present invention relates to the field of biochemical detection, and Background technique [0002] Hydrogen peroxide (H 2 O 2 ), Also known as hydrogen peroxide, is a colorless and transparent liquid having strong oxidation, corrosive. Hydrogen peroxide is often used as an important chemical product as a wide range of disinfectants, bleaches, oxidants, liquid fuels, etc. in the fields of food, medicine, defense, textile, paper, chemical synthesis, and environmental protection. The National Health and Family Planning Commission stipulates in the use of standards GB2760-2014 in food additives, and hydrogen peroxide remains are not allowed. However, some food production enterprises will use hydrogen peroxide as a cheap oxidant, bleach, super or illegal use, causing the residue of hydrogen peroxide in food, sometimes even causing food poisoning. Non-standard emissions from hydrogen peroxide production and use of companies can lead to harm to the human body ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 王菁菁刘璐崔建华董理张香丽
Owner CHANGCHUN HUILI BIOTECH CO LTD