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Microbial remediation agent and preparation method thereof

A microbial remediation and microbial technology, applied in the field of contaminated soil remediation, can solve the problems of reducing the application range of microbial soil remediation agents, single treatment mechanism, and inability to treat soil, and achieves rich microbial groups, good treatment effects, and simple and easy preparation methods. Effect

Inactive Publication Date: 2019-08-23
海盐县凌特生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the microbial soil remediation agent in the inventive method only repairs heavy metal-contaminated soil with microbial bacterial agents, and its treatment mechanism is relatively simple, and the treatment effect is relatively slight, and it cannot treat soil contaminated by organic matter, thus reducing the use of the microbial soil remediation agent. scope of application

Method used

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  • Microbial remediation agent and preparation method thereof
  • Microbial remediation agent and preparation method thereof
  • Microbial remediation agent and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0041] A microbial remediation agent comprising the following ingredients:

[0042] (a) 3,5-dinitrogeninol potassium salt as shown in formula 1;

[0043]

[0044] where M=K + ,and

[0045] (b) a mixed microbial compound bacterial agent of the genera Mycobacterium, Rhodococcus, Arthrobacter and Bacillus;

[0046] (c) polyvinyl alcohol modified nano zero-valent iron;

[0047] Wherein, in the microbial remediation agent, the weight ratio of 3,5-dinitrogenolate, microbial composite bacterial agent and polyvinyl alcohol-modified nano zero-valent iron is 0.2:1:0.5.

[0048] Microbial remediation agents can use naphthalene and phenanthrene in organic polluted soil pollutants PAHs as carbon sources for growth. Naphthalene and phenanthrene molecules are metabolized and utilized by microorganisms, and finally transformed and degraded into some low-toxic small molecules such as carbon dioxide, etc., so as to realize the Remediation of organic-contaminated soil. 3,5-Dinitrogenoxyl...

Embodiment 2

[0058] The present embodiment provides a kind of microbial remediation agent, comprises following composition:

[0059] (a) 3,5-dinitrogenoxylate as shown in formula 1;

[0060]

[0061] Among them, M=NH 4 + ;and

[0062] (b) microbial compound bacterial agent;

[0063] (c) polyvinyl alcohol modified nano zero-valent iron;

[0064] (d) microbial carbon sources; and

[0065] (e) Microbial nitrogen source.

[0066] The microbial compound bacteria agent is selected from the mixed bacteria agents of the genera Arthrobacter, Bacillus, Saccharomyces, Micrococcus, Bacillus subtilis, Flavobacterium, Pseudomonas and Alcaligenes.

[0067] In the microbial remediation agent, the weight ratio of 3,5-dinitrogenolate, microbial composite bacterial agent and polyvinyl alcohol-modified nano zero-valent iron is 1.5:1:5.

[0068] In the microbial remediation agent, the preparation method of polyvinyl alcohol-modified nanometer zero-valent iron is as follows: take 100 mL of polyvinyl a...

Embodiment 3

[0077] The present embodiment provides item [i] a kind of microbial remediation agent, comprises following composition:

[0078] (a) 3,5-dinitrogenoxylate as shown in formula 1;

[0079]

[0080] Among them, M=Li + ;and

[0081] (b) microbial compound bacterial agent;

[0082] The microbial composite bacterial agent is selected from Mycobacterium, Rhodococcus, Arthrobacter, Bacillus, Saccharomyces, Micrococcus, Bacillus subtilis, Flavobacterium, Pseudomonas and Alkagenes Mixture of Bacillus species.

[0083] The microbial remediation agent can utilize naphthalene and phenanthrene in the PAHs of organic matter-contaminated soil pollutants as carbon sources to grow, and the naphthalene and phenanthrene molecules are metabolized and utilized by microorganisms, and finally converted and degraded into some low-toxic small molecules such as carbon dioxide, etc., thereby Remediation of soil contaminated with organic matter. 3,5-Dinitrogenoxylate and naphthalene and phenanthre...

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Abstract

The invention provides a microbial remediation agent, belonging to the technical field of remediation of contaminated soil. The microbial remediation agent comprises 3,5-dinitroguaiacol salt as shownin a formula which is described in the specification, a composite microbial agent, polyvinyl alcohol modified nanometer zero-valent iron, a microbial carbon source and a microbial nitrogen source. Thepreparation method for the microbial remediation agent comprises the following steps: 1) preparing the polyvinyl alcohol modified nanometer zero-valent iron under the protection of nitrogen; 2) adding the composite microbial agent into a 3,5-dinitroguaiacol salt solution, carrying out complete mixing, and carrying out drying at a low temperature; 3) adding the polyvinyl alcohol modified nanometerzero-valent iron obtained in the step 1) into a mixture obtained in the step 2); and 4) adding the microbial carbon source and the microbial nitrogen source into a product obtained in the step 3). The microbial remediation agent provided by the invention has the following beneficial effects: the microbial remediation agent can rapidly degrade organic substances like PAHs, i.e., naphthalene, phenanthrene and the like in the contaminated soil into micromolecular substances to be absorbed and utilized by microorganisms, and can effectively remove Cr(VI) and reduce heavy-metal pollution of chromium.

Description

technical field [0001] The invention belongs to the technical field of contaminated soil remediation, and in particular relates to a microbial remediation agent and a preparation method thereof. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) are organic compounds that contain two or more benzene rings in a molecular structure and are arranged in linear, angular, or clustered shapes. It mainly comes from the incomplete utilization of fossil fuels and the volatile hydrocarbons produced by the incomplete combustion of wood, tobacco, organic polymers and other organic substances. It is one of the most harmful environmental pollutants. Phenanthrene is a model compound of PAHs, which is composed of three benzene rings arranged in a bending manner, and is one of the main components of PAHs pollutants. Environmental monitoring shows that due to the long-term and extensive use of fossil fuels, phenanthrene is widely distributed in the environment, including th...

Claims

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Application Information

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IPC IPC(8): C09K17/40B09C1/10
CPCB09C1/10C09K17/40
Inventor 董珊珊
Owner 海盐县凌特生物科技有限公司
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