Biomaterial with effects of dissolving algae, degrading algal toxin and removing nitrogen and phosphorus and preparation method and application thereof

A technology of biomaterials and algal toxins, applied in the direction of using microorganisms, biochemical equipment and methods, methods based on microorganisms, etc., can solve the problems of being easily affected by environmental factors, limited adsorption volume, pollution, etc., and achieve the solution of algal toxins Pollution problems, simple and convenient preparation process, and the effect of solving blue-green algae outbreaks

Active Publication Date: 2019-08-27
CENT SOUTH UNIV
View PDF3 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned bacteria have the following defects when dealing with algae pollution: (1) the bacteria only have the function of single algalysis or degradation of algae toxins or nitrogen and phosphorus removal; The algal toxins in the cells can effectively inhibit algae outbreaks but cause more serious algal toxin pollution, so the problem of cyanobacteria and algal toxin pollution cannot be solved simultaneously; (3) Free strains have low degradation efficiency in natural water bodies and are resistant to water flow The problems of low impact force, easy loss, low relative concentration of strains, protozoan erosion, susceptibility to environmental factors, and difficulty in realizing reuse have greatly restricted the practical application of strains.
Due to its adsorption characteristics, ACF can be used in waste gas and sewage treatment processes, but the limited adsorption volume and long-term use lead to desorption and other problems, which limit the application of ACF in the field of environmental protection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Biomaterial with effects of dissolving algae, degrading algal toxin and removing nitrogen and phosphorus and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A biological material of the present invention (such as figure 1 Shown), with the active carbon fiber-Acinetobacter composite material as the core, the surface self-assembly coating chitosan-calcium alginate polymer on the surface of the activated carbon fiber-Acinetobacter composite material; the activated carbon fiber-Acinetobacter composite material The bacillus composite material is a composite material obtained after loading Acinetobacter sp. (Acinetobacter sp., bacterial strain number is A2 (KM 926342)) with activated carbon fiber as an immobilized carrier; the chitosan-calcium alginate polymer is a shell A porous polymer sol formed by bonding and cross-linking polysaccharides and calcium alginate.

[0035] The preparation method of the biological material comprises the following steps:

[0036] (1) Acinetobacter domestication culture: Weigh 1.0g peptone, 1.0g NaCl, 0.5g beef extract into a 100ml Erlenmeyer flask, add ultrapure water to 100ml, stir, adjust pH=7.2...

Embodiment 2

[0049] A kind of biological material of the present invention, with active carbon fiber-Acinetobacter composite material as core, self-assembly coating chitosan-calcium alginate polymer on the surface of described active carbon fiber-Acinetobacter composite material; The fiber-Acinetobacter composite material is a composite material obtained after the activated carbon fiber is used as an immobilized carrier to load Acinetobacter sp. (Acinetobacter sp., strain number is A2 (KM 926342)); the chitosan-calcium alginate polymer The product is a porous polymer sol formed by bonding and cross-linking chitosan and calcium alginate.

[0050] The preparation method of the biological material comprises the following steps:

[0051] (1) Acinetobacter domestication culture: Weigh 1.0g peptone, 1.0g NaCl, 0.5g beef extract into a 100ml Erlenmeyer flask, add ultrapure water to 100ml, stir, adjust pH=7.2, and obtain beef extract peptone Liquid culture medium; add 10mL of 1μg / mL cyanotoxin aq...

Embodiment 3

[0062] A kind of biological material of the present invention, with active carbon fiber-Acinetobacter composite material as core, self-assembly coating chitosan-calcium alginate polymer on the surface of described active carbon fiber-Acinetobacter composite material; The fiber-Acinetobacter composite material is a composite material obtained after the activated carbon fiber is used as an immobilized carrier to load Acinetobacter sp. (Acinetobacter sp., strain number is A2 (KM 926342)); the chitosan-calcium alginate polymer The product is a porous polymer sol formed by bonding and cross-linking chitosan and calcium alginate.

[0063] The preparation method of the biological material comprises the following steps:

[0064] (1) Acinetobacter domestication culture: Weigh 1.0g peptone, 1.0g NaCl, 0.5g beef extract into a 100ml Erlenmeyer flask, add ultrapure water to 100ml, stir, adjust pH=7.2, and obtain beef extract peptone Liquid culture medium; add 10mL of 1.2μg / mL cyanotoxin ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a biomaterial with the effects of dissolving algae, degrading algal toxin and removing nitrogen and phosphorus, and a preparation method of the biological material. An activated carbon fiber-Acinetobacter sp. composite material is taken as a core, and the surface of the activated carbon fiber-Acinetobacter sp. composite material is coated with a chitosan-calcium alginate polymer by self-assembly; the activated carbon fiber-acinetobacter composite material is a composite material obtained by taking activated carbon fiber as an immobilized carrier to load Acinetobacter sp.; the chitosan-calcium alginate polymer is porous polymer sol formed by bonding and cross-linking chitosan and calcium alginate. The biomaterial can dissolve algae cells, degrade algal toxin, and remove nitrogen and phosphorus at the same time, furthermore has the advantages of high degradation efficiency, environmental friendliness, strong environmental tolerance, good stability, reusability, good storage, and the like, and can effectively solve the problems of cyanobacteria bloom and algal toxin pollution caused by water eutrophication.

Description

technical field [0001] The invention relates to the fields of wastewater treatment, cyanobacteria bloom, and algal toxin pollution control, in particular to a biological material capable of dissolving algae, degrading algae toxin, and removing nitrogen and phosphorus, and a preparation method and application thereof. Background technique [0002] Improper discharge of domestic sewage, industrial and agricultural wastewater, and pollution of aquaculture, excessive nitrogen and phosphorus content in water bodies, and aggravated eutrophication have led to continuous occurrence of cyanobacteria algae blooms and red tides. Microcystins are a class of monocyclic heptapeptide hepatotoxins produced by cyanobacteria, which are extremely harmful to different organs such as the liver, intestines, brain, kidneys, lungs and hearts of humans and animals. The pollution of microcystins It poses a huge threat to natural ecosystems and public health. Algae toxins have a ring structure and st...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N11/14C12N11/10C12N11/04C12N1/36C02F3/34C12R1/01
CPCC12N11/14C12N11/10C12N11/04C12N1/36C02F3/34
Inventor 丁萍何兴候杨飞任国峰伍翩陈翠梅开天瀚李晶
Owner CENT SOUTH UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products