Fluorescence probe for detecting cyanide ions and preparation method and application of fluorescence probe

Abstract

The invention relates to a fluorescence probe for detecting cyanide ions and a preparation method and application of the fluorescence probe. The fluorescence probe is a cyanide ion fluorescence probetaking boron dipyrromethene (BODIPY) as a fluorescence signal group and taking an activated CH group in a benzyl cyanide group as a recognition site, and the probe is obtained through a nucleophilic substitution reaction of 3,5-dibromo8-phenyl boron fluoride dipyrrole and benzyl cyanide. Through the research of an ultraviolet absorption and a fluorescence emission spectroscopy method, it is foundthat the probe can identify the cyanide ions singly, the lowest detectable limit in a 70% aqueous solution can reach 148 nM, and the recognition process is not disturbed by other anions. Compared withthe prior art, the fluorescence probe has the advantages that the synthesis method is simple, the raw materials are easy to obtain, the cost is lower, the selectivity to the cyanide ions is good, thesensitivity is high, reversibility is provided, the detection can be performed in the aqueous solution, and therefore the fluorescence probe has a good application prospect in the detection of the cyanide ions.

Description

technical field [0001] The invention relates to the field of fluorescent probes, in particular to a fluorescent probe for detecting cyanide ions, a preparation method and application thereof. Background technique [0002] Anions widely exist in nature and organisms, and play an important role in environmental science, clinical medicine, chemistry, biology and other fields. Among the many anions, cyanide ion has extremely high toxicity to the human body. The poisoning mechanism is that the cyanide ion can complex with the ferric ion of cytochrome oxidase, resulting in the loss of the ability of the ferric iron to transfer electrons, making the respiratory chain Interruption, eventually leading to human death due to lack of oxygen. In addition, cyanide ion also plays a very important role in synthetic fibers, leather products, metallurgical industry and electroplating technology, but the extensive use of cyanide in industrial production will inevitably cause harm to organisms...

AI Technical Summary

Problems solved by technology

At present, people have designed and synthesized many anion fluorescent probes with potential application value, but most of the probes are complicated to synthesize, high in cost, cannot be reproducibly identified, and it is difficult to identify anions in aqueous solution.

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