Keratin nano-flower material loaded with silver phosphate and preparation and application thereof

A technology of keratin and nanoflowers, applied in organic compound/hydride/coordination complex catalysts, color/spectral characteristic measurement, physical/chemical process catalysts, etc., can solve inaccurate physical and chemical properties, complex synthesis process , small amount of synthesis, etc., to achieve the effect of simple and efficient preparation method, good selectivity and high recovery rate

Active Publication Date: 2019-09-17
SHANGHAI UNIV OF ENG SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still many defects in the application of nanomaterial-based artificial mimic enzymes in biocatalysis, such as smal

Method used

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  • Keratin nano-flower material loaded with silver phosphate and preparation and application thereof
  • Keratin nano-flower material loaded with silver phosphate and preparation and application thereof
  • Keratin nano-flower material loaded with silver phosphate and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Such as figure 1 Shown, Keratin-nanoflower@Ag 3 PO 4 The synthesis process is as follows:

[0040] (1) Preparation of Keratin-nanoflower nanoflowers

[0041] ① Accurately weigh 3mg of keratin and dissolve it in PBS phosphate buffer solution (0.1M, pH=7.4);

[0042] ② After mixing evenly, add 200μL (120mM) CuSO to the above solution 4 Solution, rotate and shake the solution, then transfer the centrifuge tube to an electric heating constant temperature water tank at 25°C, and let it settle naturally for 72 hours;

[0043] ③ After centrifuging at 8000rpm for 3 minutes, remove the supernatant, wash the precipitate three times with distilled water, and finally place the obtained precipitate in a vacuum drying oven at 35°C for 12 hours.

[0044] (2) Keratin-nanofower@Ag 3 PO 4 preparation of

[0045] ① Accurately weigh the Keratin-nanoflower (5 mg) synthesized above and disperse it in deionized water;

[0046] ②Weigh again 15mg AgNO 3 dispersed in deionized water; ...

Embodiment 2

[0053] To Keratin-nanoflower@Ag 3 PO 4 The conditions for catalyzing the oxidation of its substrate by hydrogen peroxide were optimized, among which, Keratin-nanoflower@Ag 3 PO 4 Schematic diagram of colorimetric detection of uric acid figure 2 shown.

[0054] Leverage Keratin-nanoflower@Ag 3 PO 4 The steps of colorimetric detection of uric acid are as follows:

[0055]

Embodiment 21

[0057] Effect of reaction temperature on Keratin-nanoflower@Ag 3 PO 4 Solution Colorimetric Detection of Uric Acid Effect

[0058] (1) Take 360 ​​μL of acetic acid-sodium acetate buffer solution (0.2M, pH=4.00) in a 1.5mL centrifuge tube, and add 20 μL of Keratin-nanoflower@Ag to the centrifuge tube in turn 3 PO 4 (2.5mg / mL), 20 μL hydrogen peroxide aqueous solution (5.0mM), 3,3',5,5'-tetramethylbenzidine (TMB, 2.0mM), mix the above solutions evenly;

[0059] (2) Take part of the mixed solution obtained in step (1) and react in a water bath (20°C, 30°C, 40°C, 50°C, 60°C, 70°C, 80°C) for 8 minutes;

[0060] (3) Keratin-nanoflower@Ag was passed through a centrifuge 3 PO 4 Separated from the reaction solution;

[0061] (4) Measure the ultraviolet absorption spectrum of the above mixed solution with an ultraviolet-visible absorption spectrophotometer.

[0062] Experimental results such as Image 6 As shown, it can be seen from the figure that the absorbance at 652nm increa...

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Abstract

The invention relates to a keratin nano-flower material loaded with silver phosphate and preparation and application thereof. The preparation method of the keratin nano-flower material comprises the preparation of keratin nano-flowers and loading of the silver phosphate. The material is used as a catalyst for the detection of uric acid. Compared with the prior art, the material has the advantages of simple preparation process, good operability, high recovery rate, environmental protection and high efficiency, and is easy to be separated from a solution, and keratine-nano flower @ Ag3PO4 has excellent peroxidase-like catalytic activity, and can be used as a novel simulated enzyme to replace hydrogen peroxide oxidase and be widely used in biological detection, clinical diagnosis and immune analysis. The keratine-nano flower @ Ag3PO4 is used for colorimetric detection of the uric acid in serum, the detection process is simple and fast, economical and environment-friendly, and the colorimetric detection of the uric acid does not need to be marked, the detection range is wide, and the selectivity is good; even when the concentration of interfering substances is 3.3 times of the concentration of the uric acid, the keratin nano-flower material can still detect uric acid efficiently.

Description

technical field [0001] The invention belongs to the technical field of uric acid detection, and relates to a keratin nanoflower material loaded with silver phosphate and its preparation and application. Background technique [0002] There are currently more than 200 methods for the determination of uric acid, which are mainly divided into chemical reagent colorimetry, enzyme analysis, fluorescence, capillary electrophoresis, electrochemical, chemiluminescence and high performance liquid chromatography. However, these methods face insurmountable shortcomings due to expensive instruments, complex sample preparation process, and long time-consuming. Among them, the high-performance liquid chromatography requires special equipment, the detection time is long, and there are many interference factors in the sample, which makes the separation effect poor; while the chemical reagent colorimetric method has complicated operation steps, especially the pretreatment process of the sampl...

Claims

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Application Information

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IPC IPC(8): B01J31/26B01J35/10G01N21/33
CPCB01J31/26B01J35/1014B01J35/1061G01N21/33
Inventor 曹田田郑静张敏
Owner SHANGHAI UNIV OF ENG SCI
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