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Production method of quinoa polysaccharides and polypeptides

The technology of a quinoa polysaccharide and a production method, which is applied in the field of quinoa polysaccharide polypeptide production, can solve the problems of difficult removal of small-molecule impurities, affecting the efficiency of enzymatic hydrolysis, and low yield of polypeptides, and achieves the effects of increasing protein yield and improving nutritional value.

Active Publication Date: 2019-09-17
中藜农业科技(江苏)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this preparation method has the following problems: quinoa has large particles, which affects the enzymatic hydrolysis efficiency, and a large amount of acid and alkali pollutes the environment, and at the same time loses all water-soluble polysaccharides that are beneficial to the human body, and a large amount of protein components will be lost in the acid precipitation step. According to the experimental results, the protein content of quinoa lost by acid precipitation can reach 18% of the protein extracted by quinoa, but the actual crude protein obtained by acid precipitation is only 62% of the total protein of quinoa, and the actual peptide yield after hydrolysis is even lower
However, this preparation method has the following problems: the protease hydrolysis directly uses the pulverized quinoa grains as the substrate, which affects the enzymatic hydrolysis efficiency and the yield of quinoa peptides, and relevant indicators such as the degree of proteolysis or peptide yield have not been published. At the same time, it contains a lot of impurities. According to the experimental results, the extraction rate of quinoa protein by direct enzymatic hydrolysis is only 54% of the total protein of quinoa, and small molecular impurities are extremely difficult to remove or the removal cost is high

Method used

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  • Production method of quinoa polysaccharides and polypeptides
  • Production method of quinoa polysaccharides and polypeptides
  • Production method of quinoa polysaccharides and polypeptides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Alkaline extraction of quinoa protein

[0034] Wash 3.8kg of quinoa, beat through a 100-mesh sieve, add water until the ratio of solid to liquid is 1:11.5, use 27°C, pH11 for 2 hours of leaching, centrifuge, and continue to produce starch in solid phase to obtain 1.86kg of starch , the liquid phase is clarified by ceramic membranes. Alkaline-extracted protein accounts for 70% of the total protein of quinoa according to Kjeldahl nitrogen determination, and the ceramic membrane flux is 80-120L / m 2 h.

[0035] (2) Amylase treatment

[0036] Adjust the pH of the ceramic membrane clarified solution to 6.4, heat to 50°C, and add 19g of medium-temperature α-amylase for enzymatic hydrolysis for 2 hours.

[0037] (3) Ultrafiltration membrane treatment

[0038] The amylase hydrolyzate was subjected to 5000 ultrafiltration membrane treatment. Both the retentate and the permeate were subjected to Kjeldahl nitrogen determination to obtain a protein retention rate of 85% and...

Embodiment 2

[0046] (1) Alkaline extraction of quinoa protein

[0047]Wash 3.8kg of quinoa, beat through a 100-mesh sieve, add water until the ratio of solid to liquid is 1:12, use 35°C, pH11 to extract for 3 hours, centrifuge, and continue to produce starch in solid phase to obtain 1.91kg of starch , the liquid phase is clarified by ceramic membranes. The protein extraction rate calculated by Kjeldahl nitrogen determination is 81.96%, and the ceramic membrane flux is 80-120L / ㎡·h.

[0048] (2) Amylase treatment

[0049] The ceramic membrane clarified solution was adjusted to pH 7, heated to 60° C., and 25 g of medium-temperature α-amylase was added for enzymatic hydrolysis for 2 hours.

[0050] (3) Ultrafiltration membrane treatment

[0051] The amylase hydrolyzate was subjected to 5000 ultrafiltration membrane treatment. Protein retention rate 90%, membrane flux 6.4-10.7L / m 2 h.

[0052] (4) Protease hydrolysis

[0053] Adjust the pH of the 5000 ultrafiltration membrane retentate t...

Embodiment 3

[0060] Embodiment 3: the influence of alkali extraction condition

[0061] Alkaline extraction of quinoa protein by changing step (1) in Example 2: the alkaline extraction conditions are shown in Table 2, and other conditions are the same as in Example 1.

[0062] Table 2 Effects of different alkaline extraction conditions on the extraction rate of starch and protein

[0063]

[0064] Embodiment 3: the influence of ultrafiltration membrane

[0065] Referring to step (3) ultrafiltration membrane treatment in Example 1: ultrafiltration membranes with different molecular weights are used for treatment, and the obtained data are shown in Table 4. Ultrafiltration can remove the small molecular impurities in the alkali extraction supernatant, which account for 80% of the protein quality of the alkali extraction, including reducing sugar and dextrin produced by hydrolyzing starch, and purify the protein and polysaccharide retained in the retentate. The inventors have found throu...

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Abstract

The invention discloses a production method of quinoa polysaccharides and polypeptides, and belongs to the field of foods. The production method of the quinoa polysaccharides and polypeptides comprises the following steps: performing alkali extraction on quinoa, and carrying out solid-liquid separation so as to obtain a solid phase used for producing attached products, such as starch cellulose and the like, and a liquid phase; clarifying the liquid phase by using ceramic membrane, carrying out hydrolysis by using amylase, and removing low-molecular-weight impurities by using ultrafiltration membrane so as to obtain a ultra-filtration membrane concentrate; performing hydrolysis on the ultra-filtration membrane concentrate by using amylase again, carrying out concentration by using nano-filtration membrane, and carrying out desalination; and then, carrying out drying so as to obtain quinoa polysaccharide and polypeptide products. By adopting the production method of the quinoa polysaccharides and polypeptides, the degree of hydrolysis can be up to 18.5%; the produced quinoa polypeptides are small in molecular weight that the molecular weight of 99.57% of the produced quinoa polypeptides is smaller than 2400, so that, the quinoa polypeptides are easy for the human body to absorb and high in protein extraction rate; and the content of the quinoa polysaccharides are high, so that, nutritional values of the products are raised. Moreover, the quinoa polysaccharides have bacteriostatic effects on staphylococcus aureus and Escherichia coli. Compared with conventional alkali extraction and acid precipitation methods, the production method of the quinoa polysaccharides and polypeptides avoids use of large amounts of acids, and synchronously retains quinoa polysaccharides and proteins which loss in acid precipitation steps; and thus, the production method of the quinoa polysaccharides and polypeptides is more efficient, and capable of achieving higher purity compared with an enzymatic hydrolysis method.

Description

technical field [0001] The invention relates to a production method of quinoa polysaccharide polypeptide, which belongs to the field of food. Background technique [0002] With the development of social economy, the improvement of productivity and the improvement of people's living standards, people's demand for healthy food is increasingly urgent. Contemporary people have long-term irregular work and rest and unhealthy diet, resulting in decreased digestion and absorption capacity. The staple food they often eat is rich in carbohydrates, but lacks high-quality protein. Therefore, there is an urgent need for an easy-to-absorb food rich in essential amino acids. [0003] Quinoa is the most suitable "whole nutritional food" recognized by the Food and Agriculture Organization of the United Nations. It is called the lost ancient "nutrition gold", "super grain" and "future food" by international nutritionists. Respected as the "King of Vegetarian Food". Quinoa protein contains ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C12P21/06C08B37/00C08B30/04
CPCC07K1/145C12P21/06C08B37/0003C08B30/042Y02P60/87
Inventor 杨严俊朱晓军唐子萧苏宇杰
Owner 中藜农业科技(江苏)有限公司