Method using thallus whole cells to catalyze arginine to convert into agmatine

A technology of agmatine and whole cells, which is applied in the field of bioengineering, can solve the problems of cell crushing, purification and extraction, and increase the burden, and achieve the effects of high conversion efficiency, easy crystallization and purification, and short fermentation cycle
CN110257448AActive Publication Date: 2019-09-20JINAN GUOLI BIOLOGICAL SCI & TECH +1
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
JINAN GUOLI BIOLOGICAL SCI & TECH
Publication Date
2019-09-20

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Abstract

The method relates to a method using thallus whole cells to catalyze arginine to convert into agmatine and belongs to the technical field of bioengineering. The method includes: preparing whole-cell arginine decarboxylase concentrate, mixing the whole-cell arginine decarboxylase concentrate with initial conversion liquid, converting, crystalizing and recrystallizing. The method has the advantages that by controlling the carbon source, nitrogen source and dissolved oxygen during fermentation induction, the obtained arginine decarboxylase genetically engineered thallus whole-cell concentrate is high in arginine catalytic conversion efficiency; the concentrate does not contain byproducts, so that the agmatine obtained by conversion can be conveniently crystalized and purified; the thallus whole-cell concentrate is short in fermentation period, a large amount of the thallus whole-cell concentrate can be obtained in a short time, and large-scale preparation of the agmatine is benefited; agmatine product concentration on conversion liquid is 200-300g / L, conversion rate is 92-99%, agmatine product purity can reach 97-100%, and product yield can reach 90-100%.
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Description

technical field

[0001] The invention relates to a method for converting arginine into agmatine by catalyzing whole cells of bacteria, belonging to the technical field of bioengineering. Background technique

[0002] As an important biogenic amine, agmatine has great physiological functions and medical value. At present, the preparation of agmatine in my country mainly comes from chemical methods. The chemical synthesis of agmatine has cumbersome steps, low efficiency, high cost, heavy pollution, and does not meet the requirements of environmental protection. The use of biological methods to synthesize agmatine has attracted people's attention because of its advantages of fast reaction rate, simple operation, and no pollution.

[0003] Agmatine is produced by the decarboxylation of the substrate arginine catalyzed by arginine decarboxylase (see figure 1 ). The concentration of agmatine in the blood is 0.2-0.4ng / ml, and agmatine is distributed in almost all organs in the bo...

Claims

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